دورية أكاديمية
Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices.
| العنوان: | Observing Islet Function and Islet-Immune Cell Interactions in Live Pancreatic Tissue Slices. |
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| المؤلفون: | Huber MK; Department of Pathology, Immunology, and Laboratory Medicine, University of Florida., Drotar DM; Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden; Institute of Physiology, Faculty of Medicine, Technische Universität Dresden; German Center for Diabetes Research (DZD)., Hiller H; Department of Pathology, Immunology, and Laboratory Medicine, University of Florida., Beery ML; Department of Pathology, Immunology, and Laboratory Medicine, University of Florida., Joseph P; Department of Pathology, Immunology, and Laboratory Medicine, University of Florida., Kusmartseva I; Department of Pathology, Immunology, and Laboratory Medicine, University of Florida., Speier S; Paul Langerhans Institute Dresden (PLID) of the Helmholtz Zentrum München at the University Clinic Carl Gustav Carus of Technische Universität Dresden; Institute of Physiology, Faculty of Medicine, Technische Universität Dresden; German Center for Diabetes Research (DZD)., Atkinson MA; Department of Pathology, Immunology, and Laboratory Medicine, University of Florida., Mathews CE; Department of Pathology, Immunology, and Laboratory Medicine, University of Florida., Phelps EA; J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida; ephelps@bme.ufl.edu. |
| المصدر: | Journal of visualized experiments : JoVE [J Vis Exp] 2021 Apr 12 (170). Date of Electronic Publication: 2021 Apr 12. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media |
| اللغة: | English |
| بيانات الدورية: | Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]- |
| مواضيع طبية MeSH: | Cell Communication*, Diabetes Mellitus, Type 2/*pathology , Immune System/*metabolism , Islets of Langerhans/*metabolism , Pancreas/*physiology, Animals ; Diabetes Mellitus, Type 2/immunology ; Diabetes Mellitus, Type 2/metabolism ; Immune System/cytology ; Islets of Langerhans/cytology ; Mice |
| مستخلص: | Live pancreatic tissue slices allow for the study of islet physiology and function in the context of an intact islet microenvironment. Slices are prepared from live human and mouse pancreatic tissue embedded in agarose and cut using a vibratome. This method allows for the tissue to maintain viability and function in addition to preserving underlying pathologies such as type 1 (T1D) and type 2 diabetes (T2D). The slice method enables new directions in the study of the pancreas through the maintenance of the complex structures and various intercellular interactions that comprise the endocrine and exocrine tissues of the pancreas. This protocol demonstrates how to perform staining and time-lapse microscopy of live endogenous immune cells within pancreatic slices along with assessments of islet physiology. Further, this approach can be refined to discern immune cell populations specific for islet cell antigens using major histocompatibility complex-multimer reagents. |
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| معلومات مُعتمدة: | P01 AI042288 United States AI NIAID NIH HHS; T32 DK108736 United States DK NIDDK NIH HHS; R01 DK127497 United States DK NIDDK NIH HHS; UG3 DK122638 United States DK NIDDK NIH HHS; UC4 DK104194 United States DK NIDDK NIH HHS; R01 DK123292 United States DK NIDDK NIH HHS |
| تواريخ الأحداث: | Date Created: 20210426 Date Completed: 20210614 Latest Revision: 20220413 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8314551 |
| DOI: | 10.3791/62207 |
| PMID: | 33900291 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-087X |
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| DOI: | 10.3791/62207 |