دورية أكاديمية
أعرض في EDS

Analysis of cell-type-specific chromatin modifications and gene expression in Drosophila neurons that direct reproductive behavior.

التفاصيل البيبلوغرافية
العنوان: Analysis of cell-type-specific chromatin modifications and gene expression in Drosophila neurons that direct reproductive behavior.
المؤلفون: Palmateer CM; Department of Biomedical Sciences, Florida State University, College of Medicine, Tallahassee, Florida, United States of America., Moseley SC; Department of Biomedical Sciences, Florida State University, College of Medicine, Tallahassee, Florida, United States of America., Ray S; Department of Biomedical Sciences, Florida State University, College of Medicine, Tallahassee, Florida, United States of America., Brovero SG; Department of Biomedical Sciences, Florida State University, College of Medicine, Tallahassee, Florida, United States of America., Arbeitman MN; Department of Biomedical Sciences, Florida State University, College of Medicine, Tallahassee, Florida, United States of America.; Program of Neuroscience, Florida State University, Tallahassee, Florida, United States of America.
المصدر: PLoS genetics [PLoS Genet] 2021 Apr 26; Vol. 17 (4), pp. e1009240. Date of Electronic Publication: 2021 Apr 26 (Print Publication: 2021).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101239074 Publication Model: eCollection Cited Medium: Internet ISSN: 1553-7404 (Electronic) Linking ISSN: 15537390 NLM ISO Abbreviation: PLoS Genet Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science, c2005-
مواضيع طبية MeSH: Chromatin/*genetics , Drosophila Proteins/*genetics , Nerve Tissue Proteins/*genetics , Neurons/*metabolism , Sexual Behavior, Animal/*physiology , Transcription Factors/*genetics, Animals ; Cell Lineage/genetics ; Chromatin Assembly and Disassembly/genetics ; Drosophila melanogaster/genetics ; Drosophila melanogaster/growth & development ; Gene Expression Regulation, Developmental/genetics ; Pupa/genetics ; Pupa/growth & development ; RNA-Seq
مستخلص: Examining the role of chromatin modifications and gene expression in neurons is critical for understanding how the potential for behaviors are established and maintained. We investigate this question by examining Drosophila melanogaster fru P1 neurons that underlie reproductive behaviors in both sexes. We developed a method to purify cell-type-specific chromatin (Chromatag), using a tagged histone H2B variant that is expressed using the versatile Gal4/UAS gene expression system. Here, we use Chromatag to evaluate five chromatin modifications, at three life stages in both sexes. We find substantial changes in chromatin modification profiles across development and fewer differences between males and females. Additionally, we find chromatin modifications that persist in different sets of genes from pupal to adult stages, which may point to genes important for cell fate determination in fru P1 neurons. We generated cell-type-specific RNA-seq data sets, using translating ribosome affinity purification (TRAP). We identify actively translated genes in fru P1 neurons, revealing novel stage- and sex-differences in gene expression. We also find chromatin modification enrichment patterns that are associated with gene expression. Next, we use the chromatin modification data to identify cell-type-specific super-enhancer-containing genes. We show that genes with super-enhancers in fru P1 neurons differ across development and between the sexes. We validated that a set of genes are expressed in fru P1 neurons, which were chosen based on having a super-enhancer and TRAP-enriched expression in fru P1 neurons.
Competing Interests: The authors have declared that no competing interests exist.
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معلومات مُعتمدة: P40 OD018537 United States OD NIH HHS; R01 GM073039 United States GM NIGMS NIH HHS; R01 GM116998 United States GM NIGMS NIH HHS
المشرفين على المادة: 0 (Chromatin)
0 (Drosophila Proteins)
0 (Nerve Tissue Proteins)
0 (Transcription Factors)
0 (fru protein, Drosophila)
تواريخ الأحداث: Date Created: 20210426 Date Completed: 20210827 Latest Revision: 20210827
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8102012
DOI: 10.1371/journal.pgen.1009240
PMID: 33901168
قاعدة البيانات: MEDLINE
الوصف
تدمد:1553-7404
DOI:10.1371/journal.pgen.1009240