دورية أكاديمية
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Activation of transient receptor potential vanilloid channel 4 contributes to the development of ethanol-induced gastric injury in mice.

التفاصيل البيبلوغرافية
العنوان: Activation of transient receptor potential vanilloid channel 4 contributes to the development of ethanol-induced gastric injury in mice.
المؤلفون: Pacheco G; Biotechnology and Biodiversity Center Research (BIOTEC), Post-graduation Program in Biotechnology, Federal University of the Parnaíba Delta (UFDPar), Parnaíba, PI, Brazil., Oliveira AP; The Northeastern Biotechnology Network (RENORBIO), Federal University of Piauí (UFPI), Teresina, PI, Brazil., Noleto IRSG; The Northeastern Biotechnology Network (RENORBIO), Federal University of Piauí (UFPI), Teresina, PI, Brazil., Araújo AK; Biotechnology and Biodiversity Center Research (BIOTEC), Post-graduation Program in Biotechnology, Federal University of the Parnaíba Delta (UFDPar), Parnaíba, PI, Brazil., Lopes ALF; Biotechnology and Biodiversity Center Research (BIOTEC), Post-graduation Program in Biotechnology, Federal University of the Parnaíba Delta (UFDPar), Parnaíba, PI, Brazil., Sousa FBM; The Northeastern Biotechnology Network (RENORBIO), Federal University of Piauí (UFPI), Teresina, PI, Brazil., Chaves LS; Biotechnology and Biodiversity Center Research (BIOTEC), Post-graduation Program in Biotechnology, Federal University of the Parnaíba Delta (UFDPar), Parnaíba, PI, Brazil., Alves EHP; Biotechnology and Biodiversity Center Research (BIOTEC), Post-graduation Program in Biotechnology, Federal University of the Parnaíba Delta (UFDPar), Parnaíba, PI, Brazil., Vasconcelos DFP; Biotechnology and Biodiversity Center Research (BIOTEC), Post-graduation Program in Biotechnology, Federal University of the Parnaíba Delta (UFDPar), Parnaíba, PI, Brazil; The Northeastern Biotechnology Network (RENORBIO), Federal University of Piauí (UFPI), Teresina, PI, Brazil., Araujo AR; Biotechnology and Biodiversity Center Research (BIOTEC), Post-graduation Program in Biotechnology, Federal University of the Parnaíba Delta (UFDPar), Parnaíba, PI, Brazil., Nicolau LD; Biotechnology and Biodiversity Center Research (BIOTEC), Post-graduation Program in Biotechnology, Federal University of the Parnaíba Delta (UFDPar), Parnaíba, PI, Brazil., Magierowski M; Gaseous Mediators and Experimental Gastroenterology Laboratory, Department of Physiology, Jagiellonian University Medical College, Cracow, Poland., Medeiros JVR; Biotechnology and Biodiversity Center Research (BIOTEC), Post-graduation Program in Biotechnology, Federal University of the Parnaíba Delta (UFDPar), Parnaíba, PI, Brazil; The Northeastern Biotechnology Network (RENORBIO), Federal University of Piauí (UFPI), Teresina, PI, Brazil. Electronic address: jandvenes@ufpi.edu.br.
المصدر: European journal of pharmacology [Eur J Pharmacol] 2021 Jul 05; Vol. 902, pp. 174113. Date of Electronic Publication: 2021 Apr 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 1254354 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0712 (Electronic) Linking ISSN: 00142999 NLM ISO Abbreviation: Eur J Pharmacol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2005- : Amsterdam : Elsevier Science
Original Publication: Amsterdam, North Holland Pub. Co.
مواضيع طبية MeSH: Stomach Ulcer/*metabolism , Stomach Ulcer/*physiopathology , TRPV Cation Channels/*agonists , TRPV Cation Channels/*metabolism, Animals ; Edema/chemically induced ; Edema/metabolism ; Ethanol/toxicity ; Gastric Mucosa/drug effects ; Gastric Mucosa/injuries ; Gastric Mucosa/metabolism ; Glutathione/metabolism ; Leucine/analogs & derivatives ; Leucine/pharmacology ; Leucine/therapeutic use ; Male ; Malondialdehyde/metabolism ; Mice ; Oxidative Stress/drug effects ; Piperidines/pharmacology ; Piperidines/therapeutic use ; Quinolines/pharmacology ; Quinolines/therapeutic use ; Ruthenium Red/pharmacology ; Ruthenium Red/therapeutic use ; Stomach Ulcer/chemically induced ; Stomach Ulcer/pathology ; Sulfonamides/pharmacology ; Sulfonamides/therapeutic use ; Superoxide Dismutase/metabolism ; TRPV Cation Channels/antagonists & inhibitors ; TRPV Cation Channels/genetics ; Up-Regulation/drug effects
مستخلص: The transient receptor potential vanilloid channel 4 (TRPV4) is associated with the development of several pathologies, particularly gastric disorders. However, there are no studies associating this receptor with the pathophysiology of gastric erosions. The aim of this study was to investigate the role of TRPV4 in the development of ethanol-induced gastric damage in vivo. Gastric lesions were induced by ethanol in Swiss mice pretreated with TRPV4 antagonists, GSK2193874 (0.1; 0.3 and 0.9 mg/kg) or Ruthenium red (0.03; 0.1 or 0.3 mg/kg) or its agonist, GSK1016790A (0.9 mg/kg). Gastric mucosal samples were taken for histopathology, immunohistochemistry, atomic force microscopy and evaluation of antioxidant parameters. The gastric mucus content and TRPV4 mRNA expression were analyzed. Ethanol exposure induced upregulation of gastric mRNA and protein expression of TRPV4. TRPV4 blockade promoted gastroprotection against ethanol-induced injury on macro- and microscopic levels, leading to reduced hemorrhage, cell loss and edema and enhanced gastric mucosal integrity. Moreover, an increase in superoxide dismutase (SOD) and glutathione (GSH) activity was observed, followed by a decrease in malondialdehyde (MDA) levels. TRPV4 blockade during alcohol challenge reestablished gastric mucus content. The combination of TRPV4 agonist and ethanol revealed macroscopic exacerbation of gastric damage area. Our results confirmed the association of TRPV4 with the development of gastric injury, showing the importance of this receptor for further investigations in the field of gastrointestinal pathophysiology and pharmacology.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Vanilloid receptors. gastric damage. Mucosal inflammation. TRPV4
المشرفين على المادة: 0 (GSK2193874)
0 (N-(1-((4-(2-(((2,4-dichlorophenyl)sulfonyl)amino)-3-hydroxypropanoyl)-1-piperazinyl)carbonyl)-3-methylbutyl)-1-benzothiophene-2-carboxamide)
0 (Piperidines)
0 (Quinolines)
0 (Sulfonamides)
0 (TRPV Cation Channels)
0 (Trpv4 protein, mouse)
11103-72-3 (Ruthenium Red)
3K9958V90M (Ethanol)
4Y8F71G49Q (Malondialdehyde)
EC 1.15.1.1 (Superoxide Dismutase)
GAN16C9B8O (Glutathione)
GMW67QNF9C (Leucine)
تواريخ الأحداث: Date Created: 20210426 Date Completed: 20210927 Latest Revision: 20210927
رمز التحديث: 20240628
DOI: 10.1016/j.ejphar.2021.174113
PMID: 33901460
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0712
DOI:10.1016/j.ejphar.2021.174113