دورية أكاديمية
Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats.
| العنوان: | Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats. |
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| المؤلفون: | Rütgen BC; Clinical Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Baszler E; Clinical Unit of Internal Medicine Small Animals, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria., Weingand N; Clinical Unit of Internal Medicine Small Animals, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria., Wolfesberger B; Clinical Unit of Internal Medicine Small Animals, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria., Baumgartner D; Clinical Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Hammer SE; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Groiss S; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Fuchs-Baumgartinger A; Institute of Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Saalmüller A; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Schwendenwein I; Clinical Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria. |
| المصدر: | Journal of feline medicine and surgery [J Feline Med Surg] 2022 Feb; Vol. 24 (2), pp. 77-90. Date of Electronic Publication: 2021 Apr 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Country of Publication: England NLM ID: 100897329 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2750 (Electronic) Linking ISSN: 1098612X NLM ISO Abbreviation: J Feline Med Surg Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2012- : London : Sage Original Publication: London ; Philadelphia : WB Saunders Co., |
| مواضيع طبية MeSH: | Lymph Nodes*/cytology , Lymphocyte Subsets*, Animals ; Antibodies, Monoclonal ; Cats ; Flow Cytometry/veterinary ; Immunophenotyping/veterinary ; Prospective Studies |
| مستخلص: | Objectives: Flow cytometric (FCM) immunophenotyping of lymphoid tissue aspirates is an available adjunct for feline lymphoma diagnostics. Reference data have only been established for feline peripheral blood. Studies investigating the composition of normal and mildly reactive feline lymph nodes (LNs) are lacking. The aim of this prospective study was to establish reference data for lymphocyte subpopulations in normal and mildly reactive feline peripheral LNs using a standardised multicolour panel of antibodies. Methods: Macroscopically inconspicuous mandibular and/or popliteal LNs from 31 adult cats, which were euthanased for reasons other than haematological diseases, were excised and processed within 5 h after death. Multicolour flow cytometry using eight different feline-specific, anti-canine and human cross-reactive monoclonal antibodies used in current diagnostic marker panels was performed after cytological exclusion of pathological states and complemented by lymphocyte clonality testing, histopathology and immunohistochemistry (IHC) to ensure the absence of lymphoid disease. Results: Of 31 cats, the immunophenotyping data of 24 individuals could be included as histopathology and clonality testing excluded a pathological condition. Lymphocyte populations showed the following positive antibody reactions: CD18 + 86.3% ± 13.86%, CD3 + 54.81% ± 11.10%, CD5 + 57.39% ± 12.66%, CD21 + 40.42% ± 12.40%, CD79alphacy + (CD79αcy) 30.41% ± 13.49% and CD14 + 0.75% ± 1.35%. There were 30.88% ± 13.48% CD4 + and 12.91% ± 6.68% CD8 + cells. Cytology revealed a mixed population of mostly lymphoid cells in all samples. The absence of a monoclonal/oligoclonal neoplastic population was confirmed by lymphocyte clonality testing. Histopathology and IHC showed a normal or mildly reactive pattern in all cases. Conclusions and Relevance: This study establishes FCM immunophenotyping data of lymphocyte populations of normal and mildly reactive feline peripheral LNs. For the first time, anti-CD5, CD4, CD8 and CD21 reference data in normal and mildly reactive feline peripheral LNs are presented. CD18, CD3, CD14 and CD79αcy have been used to establish reference data for the first time in any feline material. |
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| فهرسة مساهمة: | Keywords: Immunophenotyping; PARR; clonality testing; flow cytometry; lymph node; reference values |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) |
| تواريخ الأحداث: | Date Created: 20210428 Date Completed: 20220314 Latest Revision: 20240515 |
| رمز التحديث: | 20240515 |
| مُعرف محوري في PubMed: | PMC10812179 |
| DOI: | 10.1177/1098612X211005310 |
| PMID: | 33908810 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-2750 |
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| DOI: | 10.1177/1098612X211005310 |