دورية أكاديمية
أعرض في EDS

Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos.

التفاصيل البيبلوغرافية
العنوان: Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos.
المؤلفون: Husseiny WA; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt., Hassanin AAI; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt., El Nabtiti AAS; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt., Khalil K; Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt., Elaswad A; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
المصدر: Genes [Genes (Basel)] 2021 Apr 22; Vol. 12 (5). Date of Electronic Publication: 2021 Apr 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Chickens/*genetics , Gene Expression/*drug effects , Gene Expression Regulation, Developmental/*drug effects , Metal Nanoparticles/*administration & dosage , Muscle Development/*drug effects , Silver/*pharmacology, Animals ; Chick Embryo ; Muscle, Skeletal/drug effects ; Myogenic Regulatory Factors/genetics ; Myogenin/genetics
مستخلص: The present study was conducted to investigate the effects of colloidal nanoparticles of silver (Nano-Ag) on the expression of myogenesis-related genes in chicken embryos. The investigated genes included the members of the myogenic regulatory factors family ( MRFs ) and myocyte enhancer factor 2A ( MEF2A ) genes. A total of 200 fertilized broiler eggs (Indian River) were randomly distributed into four groups; non-injected control, injected control with placebo, treatment I in ovo injected with 20 ppm Nano-Ag, and treatment II in ovo injected with 40 ppm Nano-Ag. The eggs were then incubated for 21 days at the optimum temperature and humidity conditions. Breast muscle tissues were collected at the 5th, 8th, and 18th days of the incubation period. The mRNA expression of myogenic determination factor 1 ( MYOD1 ), myogenic factor 5 ( MYF5 ), myogenic factor 6 ( MYF6 ), myogenin ( MYOG ), and MEF2A was measured at the three sampling points using real-time quantitative PCR, while MYOD1 protein expression was evaluated on day 18 using western blot. Breast muscle tissues were histologically examined on day 18 to detect the changes at the cellular level. Our results indicate that myogenesis was enhanced with the low concentration (20 ppm) of Nano-Ag due to the higher expression of MYOD1 , MYF5 , and MYF6 at the transcriptional level and MYOD1 at the translational level. Moreover, histological analysis revealed the presence of hyperplasia (31.4% more muscle fibers) in treatment I (injected with 20 ppm). Our findings indicate that in ovo injection of 20 ppm Nano-Ag enhances the development of muscles in chicken embryos compared with the 40-ppm dosage and provide crucial information for the use of silver nanoparticles in poultry production.
References: Int J Nanomedicine. 2010 Dec 06;5:1085-94. (PMID: 21270959)
J Cell Physiol. 2001 Nov;189(2):189-96. (PMID: 11598904)
J Morphol. 1951 Jan;88(1):49-92. (PMID: 24539719)
Anal Biochem. 1976 May 7;72:248-54. (PMID: 942051)
Mol Cell Biol. 1998 Jan;18(1):69-77. (PMID: 9418854)
Nat Commun. 2016 Aug 03;7:12397. (PMID: 27484840)
Am J Infect Control. 1998 Dec;26(6):572-7. (PMID: 9836841)
J Biol Chem. 1990 Apr 15;265(11):5960-3. (PMID: 1690720)
Genes Dev. 1995 Jun 1;9(11):1388-99. (PMID: 7797078)
Curr Opin Genet Dev. 2013 Oct;23(5):568-73. (PMID: 23756045)
EMBO J. 1989 Dec 1;8(12):3617-25. (PMID: 2583111)
Development. 1994 Nov;120(11):3083-92. (PMID: 7720554)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
BMC Vet Res. 2018 Jan 02;14(1):1. (PMID: 29291752)
Biomaterials. 2009 Aug;30(23-24):3891-914. (PMID: 19427031)
Annu Rev Cell Dev Biol. 1998;14:167-96. (PMID: 9891782)
Nanoscale Res Lett. 2012 Jul 24;7(1):418. (PMID: 22827927)
Development. 1991 Apr;111(4):1097-107. (PMID: 1652425)
Nucleic Acids Res. 1993 May 25;21(10):2503-9. (PMID: 8389445)
Adv Mater. 2012 Jul 24;24(28):3779-802. (PMID: 22610698)
Nature. 1970 Aug 15;227(5259):680-5. (PMID: 5432063)
J Cell Biol. 2005 Nov 7;171(3):471-82. (PMID: 16275751)
Biosci Rep. 2020 Mar 27;40(3):. (PMID: 32124930)
Nature. 1993 Aug 5;364(6437):532-5. (PMID: 8393146)
Cold Spring Harb Perspect Biol. 2012 Feb 01;4(2):. (PMID: 22300977)
Genes Dev. 2006 Sep 1;20(17):2450-64. (PMID: 16951257)
Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834)
Arch Anim Nutr. 2012 Feb;66(1):1-13. (PMID: 22397092)
Animals (Basel). 2019 Oct 21;9(10):. (PMID: 31640236)
Comp Biochem Physiol A Mol Integr Physiol. 2012 Mar;161(3):315-9. (PMID: 22182492)
Arch Anim Nutr. 2013;67(5):347-55. (PMID: 23952606)
فهرسة مساهمة: Keywords: MRFs; broiler; embryogenesis; myogenesis; nanoparticles; real-time PCR
المشرفين على المادة: 0 (Myogenic Regulatory Factors)
0 (Myogenin)
0 (myogenic factor 6)
3M4G523W1G (Silver)
تواريخ الأحداث: Date Created: 20210430 Date Completed: 20210826 Latest Revision: 20210826
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8146831
DOI: 10.3390/genes12050629
PMID: 33922304
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4425
DOI:10.3390/genes12050629