دورية أكاديمية
Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos.
العنوان: | Silver Nanoparticles as Modulators of Myogenesis-Related Gene Expression in Chicken Embryos. |
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المؤلفون: | Husseiny WA; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt., Hassanin AAI; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt., El Nabtiti AAS; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt., Khalil K; Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt., Elaswad A; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt. |
المصدر: | Genes [Genes (Basel)] 2021 Apr 22; Vol. 12 (5). Date of Electronic Publication: 2021 Apr 22. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel : MDPI |
مواضيع طبية MeSH: | Chickens/*genetics , Gene Expression/*drug effects , Gene Expression Regulation, Developmental/*drug effects , Metal Nanoparticles/*administration & dosage , Muscle Development/*drug effects , Silver/*pharmacology, Animals ; Chick Embryo ; Muscle, Skeletal/drug effects ; Myogenic Regulatory Factors/genetics ; Myogenin/genetics |
مستخلص: | The present study was conducted to investigate the effects of colloidal nanoparticles of silver (Nano-Ag) on the expression of myogenesis-related genes in chicken embryos. The investigated genes included the members of the myogenic regulatory factors family ( MRFs ) and myocyte enhancer factor 2A ( MEF2A ) genes. A total of 200 fertilized broiler eggs (Indian River) were randomly distributed into four groups; non-injected control, injected control with placebo, treatment I in ovo injected with 20 ppm Nano-Ag, and treatment II in ovo injected with 40 ppm Nano-Ag. The eggs were then incubated for 21 days at the optimum temperature and humidity conditions. Breast muscle tissues were collected at the 5th, 8th, and 18th days of the incubation period. The mRNA expression of myogenic determination factor 1 ( MYOD1 ), myogenic factor 5 ( MYF5 ), myogenic factor 6 ( MYF6 ), myogenin ( MYOG ), and MEF2A was measured at the three sampling points using real-time quantitative PCR, while MYOD1 protein expression was evaluated on day 18 using western blot. Breast muscle tissues were histologically examined on day 18 to detect the changes at the cellular level. Our results indicate that myogenesis was enhanced with the low concentration (20 ppm) of Nano-Ag due to the higher expression of MYOD1 , MYF5 , and MYF6 at the transcriptional level and MYOD1 at the translational level. Moreover, histological analysis revealed the presence of hyperplasia (31.4% more muscle fibers) in treatment I (injected with 20 ppm). Our findings indicate that in ovo injection of 20 ppm Nano-Ag enhances the development of muscles in chicken embryos compared with the 40-ppm dosage and provide crucial information for the use of silver nanoparticles in poultry production. |
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فهرسة مساهمة: | Keywords: MRFs; broiler; embryogenesis; myogenesis; nanoparticles; real-time PCR |
المشرفين على المادة: | 0 (Myogenic Regulatory Factors) 0 (Myogenin) 0 (myogenic factor 6) 3M4G523W1G (Silver) |
تواريخ الأحداث: | Date Created: 20210430 Date Completed: 20210826 Latest Revision: 20210826 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC8146831 |
DOI: | 10.3390/genes12050629 |
PMID: | 33922304 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2073-4425 |
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DOI: | 10.3390/genes12050629 |