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The role of chemotaxis and efflux pumps on nitrate reduction in the toxic regions of a ciprofloxacin concentration gradient.

التفاصيل البيبلوغرافية
العنوان: The role of chemotaxis and efflux pumps on nitrate reduction in the toxic regions of a ciprofloxacin concentration gradient.
المؤلفون: Alcalde RE; Department of Civil, Architectural, and Environmental Engineering, University of Texas at Austin, Austin, TX, USA., Dundas CM; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Dong Y; School of Environmental Studies, China University of Geosciences (Wuhan), Wuhan, Hubei, China.; Department of Geology, University of Illinois at Urbana-Champaign, Urbana, IL, USA., Sanford RA; Department of Geology, University of Illinois at Urbana-Champaign, Urbana, IL, USA., Keitz BK; McKetta Department of Chemical Engineering, University of Texas at Austin, Austin, TX, USA., Fouke BW; Department of Geology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.; Department of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.; Carl R. Woese Institute of Genomic Biology, University of Illinois Urbana-Champaign, Urbana, IL, USA., Werth CJ; Department of Civil, Architectural, and Environmental Engineering, University of Texas at Austin, Austin, TX, USA. werth@utexas.edu.
المصدر: The ISME journal [ISME J] 2021 Oct; Vol. 15 (10), pp. 2920-2932. Date of Electronic Publication: 2021 Apr 29.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 101301086 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1751-7370 (Electronic) Linking ISSN: 17517362 NLM ISO Abbreviation: ISME J Subsets: MEDLINE
أسماء مطبوعة: Publication: 2024- : Oxford : Oxford University Press
Original Publication: London : Nature Pub. Group
مواضيع طبية MeSH: Ciprofloxacin*/pharmacology , Nitrates*, Anti-Bacterial Agents/pharmacology ; Bacterial Proteins ; Chemotaxis ; Drug Resistance, Multiple, Bacterial ; Ecosystem ; Membrane Transport Proteins ; Microbial Sensitivity Tests ; Shewanella
مستخلص: Spatial concentration gradients of antibiotics are prevalent in the natural environment. Yet, the microbial response in these heterogeneous systems remains poorly understood. We used a microfluidic reactor to create an artificial microscopic ecosystem that generates diffusive gradients of solutes across interconnected microenvironments. With this reactor, we showed that chemotaxis toward a soluble electron acceptor (nitrate) allowed Shewanella oneidensis MR-1 to inhabit and sustain metabolic activity in highly toxic regions of the antibiotic ciprofloxacin (>80× minimum inhibitory concentration, MIC). Acquired antibiotic resistance was not observed for cells extracted from the reactor, so we explored the role of transient adaptive resistance by probing multidrug resistance (MDR) efflux pumps, ancient elements that are important for bacterial physiology and virulence. Accordingly, we constructed an efflux pump deficient mutant (∆mexF) and used resistance-nodulation-division (RND) efflux pump inhibitors (EPIs). While batch results showed the importance of RND efflux pumps for microbial survival, microfluidic studies indicated that these pumps were not necessary for survival in antibiotic gradients. Our work contributes to an emerging body of knowledge deciphering the effects of antibiotic spatial heterogeneity on microorganisms and highlights differences of microbial response in these systems versus well-mixed batch conditions.
(© 2021. The Author(s), under exclusive licence to International Society for Microbial Ecology.)
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المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Bacterial Proteins)
0 (Membrane Transport Proteins)
0 (Nitrates)
5E8K9I0O4U (Ciprofloxacin)
SCR Organism: Shewanella oneidensis
تواريخ الأحداث: Date Created: 20210430 Date Completed: 20210922 Latest Revision: 20230201
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8443623
DOI: 10.1038/s41396-021-00975-1
PMID: 33927341
قاعدة البيانات: MEDLINE
الوصف
تدمد:1751-7370
DOI:10.1038/s41396-021-00975-1