دورية أكاديمية
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UPLC-PDA-ESI-QTOF-MS/MS fingerprint of purified flavonoid enriched fraction of Bryophyllum pinnatum ; antioxidant properties, anticholinesterase activity and in silico studies.

التفاصيل البيبلوغرافية
العنوان: UPLC-PDA-ESI-QTOF-MS/MS fingerprint of purified flavonoid enriched fraction of Bryophyllum pinnatum ; antioxidant properties, anticholinesterase activity and in silico studies.
المؤلفون: Ogidigo JO; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria.; Bioresources Development Centre, National Biotechnology Development Agency, Abuja, Nigeria., Anosike CA; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria., Joshua PE; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria., Ibeji CU; Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria., Ekpo DE; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria., Nwanguma BC; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria., Nwodo OFC; Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria.; Department of Biochemistry, Mkar University, Benue State, Nigeria.
المصدر: Pharmaceutical biology [Pharm Biol] 2021 Dec; Vol. 59 (1), pp. 444-456.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 9812552 Publication Model: Print Cited Medium: Internet ISSN: 1744-5116 (Electronic) Linking ISSN: 13880209 NLM ISO Abbreviation: Pharm Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: [London] : Taylor & Francis
Original Publication: Lisse, the Netherlands : Swets & Zeitlinger, c1998-
مواضيع طبية MeSH: Kalanchoe*, Antioxidants/*analysis , Cholinesterase Inhibitors/*analysis , Flavonoids/*analysis , Plant Extracts/*analysis , Tandem Mass Spectrometry/*methods, Acetylcholinesterase/analysis ; Antioxidants/chemistry ; Butyrylcholinesterase/analysis ; Cholinesterase Inhibitors/chemistry ; Chromatography, High Pressure Liquid/methods ; Computer Simulation ; Crystallography, X-Ray/methods ; DNA Fingerprinting/methods ; Dose-Response Relationship, Drug ; Flavonoids/chemistry ; Humans ; Plant Extracts/chemistry ; Protein Structure, Secondary ; Spectrometry, Mass, Electrospray Ionization/methods
مستخلص: Context: Bryophyllum pinnatum (Lam.) Oken (Crassulaceae) is used traditionally to treat many ailments.
Objectives: This study characterizes the constituents of B. pinnatum flavonoid-rich fraction (BPFRF) and investigates their antioxidant and anticholinesterase activity using in vitro and in silico approaches.
Materials and Methods: Methanol extract of B. pinnatum leaves was partitioned to yield the ethyl acetate fraction. BPFRF was isolated from the ethyl acetate fraction and purified. The constituent flavonoids were structurally characterized using UPLC-PDA-MS 2 . Antioxidant activity (DPPH), Fe 2+ -induced lipid peroxidation (LP) and anticholinesterase activity (Ellman's method) of the BPFRF and standards (ascorbic acid and rivastigmine) across a concentration range of 3.125-100   μg/mL were evaluated in vitro for 4 months. Molecular docking was performed to give insight into the binding potentials of BPFRF constituents against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE).
Results: UPLC-PDA-MS 2 analysis of BPFRF identified carlinoside, quercetin (most dominant), luteolin, isorhamnetin, luteolin-7-glucoside. Carlinoside was first reported in this plant. BPFRF significantly inhibited DPPH radical (IC 50 = 7.382 ± 0.79 µg/mL) and LP (IC 50 = 7.182 ± 0.60 µg/mL) better than quercetin and ascorbic acid. Also, BPFRF exhibited potent inhibition against AChE and BuChE with IC 50 values of 22.283 ± 0.27 µg/mL and 33.437 ± 1.46 µg/mL, respectively compared to quercetin and rivastigmine. Docking studies revealed that luteolin-7-glucoside, carlinoside and quercetin interact effectively with crucial amino acid residues of AChE and BuChE through hydrogen bonds.
Discussion and Conclusions: BPFRF possesses an excellent natural source of cholinesterase inhibitor and antioxidant. The material could be further explored for the potential treatment of oxidative damage and cholinergic dysfunction in Alzheimer's disease.
References: Biomed Pharmacother. 2018 May;101:860-870. (PMID: 29635895)
J Comput Aided Mol Des. 2013 Mar;27(3):221-34. (PMID: 23579614)
Planta Med. 2017 Feb;83(3-04):224-231. (PMID: 27420352)
J Nutr Sci. 2018 May 21;7:e19. (PMID: 29854398)
Biomed Rep. 2016 May;4(5):519-522. (PMID: 27123241)
Planta Med. 2015 Aug;81(12-13):1190-7. (PMID: 26132852)
Nat Prod Rep. 2019 Aug 1;36(8):1053-1092. (PMID: 30924818)
Prep Biochem Biotechnol. 2016 Jul 3;46(5):489-94. (PMID: 26445098)
Front Pharmacol. 2018 Dec 11;9:1383. (PMID: 30618732)
Biomed Chromatogr. 2020 Nov;34(11):e4933. (PMID: 32598044)
Braz J Biol. 2011 Aug;71(3):783-9. (PMID: 21881804)
Free Radic Biol Med. 2007 Aug 15;43(4):477-503. (PMID: 17640558)
J Evid Based Complementary Altern Med. 2016 Oct;21(4):NP11-7. (PMID: 26438716)
Curr Aging Sci. 2010 Feb;3(1):34-42. (PMID: 20298168)
J Ethnopharmacol. 2014 Jun 11;154(2):330-8. (PMID: 24727190)
Planta Med. 2000 Dec;66(8):687-93. (PMID: 11199122)
Curr Pharm Des. 2006;12(2):217-25. (PMID: 16454738)
J Alzheimers Dis. 2018;62(3):933-942. (PMID: 29562544)
J Agric Food Chem. 2017 Nov 29;65(47):10270-10281. (PMID: 29063755)
J Med Chem. 2006 Oct 19;49(21):6177-96. (PMID: 17034125)
J Biol Chem. 2003 Oct 17;278(42):41141-7. (PMID: 12869558)
J Med Chem. 2012 Nov 26;55(22):10282-6. (PMID: 23035744)
Pharm Biol. 2017 Dec;55(1):1875-1883. (PMID: 28629287)
J Alzheimers Dis. 2016 Sep 6;54(2):427-43. (PMID: 27567871)
J Enzyme Inhib Med Chem. 2018 Dec;33(1):936-944. (PMID: 29734888)
Oxid Med Cell Longev. 2015;2015:504678. (PMID: 26301043)
Drug Discov Ther. 2012 Dec;6(6):285-90. (PMID: 23337815)
Pharmacognosy Res. 2013 Oct;5(4):247-53. (PMID: 24174817)
Planta Med. 2013 Nov;79(16):1565-71. (PMID: 24072500)
Acta Pol Pharm. 2011 Jan-Feb;68(1):23-9. (PMID: 21485698)
Pharm Biol. 2016;54(1):118-29. (PMID: 25856713)
Molecules. 2019 Apr 05;24(7):. (PMID: 30959739)
J Ethnopharmacol. 2007 Jan 19;109(2):359-63. (PMID: 16950584)
BMC Res Notes. 2015 Oct 30;8:621. (PMID: 26518275)
Psychogeriatrics. 2014 Mar;14(1):1-10. (PMID: 24646308)
3 Biotech. 2016 Jun;6(1):109. (PMID: 28330179)
Clin Interv Aging. 2007;2(3):347-59. (PMID: 18044185)
Molecules. 2014 Oct 29;19(11):17400-21. (PMID: 25356563)
Pathophysiology. 2006 Aug;13(3):195-208. (PMID: 16781128)
Biochem Pharmacol. 1961 Jul;7:88-95. (PMID: 13726518)
Molecules. 2019 Jun 26;24(13):. (PMID: 31247911)
J Alzheimers Dis. 2017;57(4):1105-1121. (PMID: 28059794)
Biochem Pharmacol. 2014 Apr 15;88(4):529-39. (PMID: 24361915)
J Ethnopharmacol. 2018 Nov 15;226:176-184. (PMID: 30102993)
J Nutr Sci. 2016 Dec 29;5:e47. (PMID: 28620474)
BMC Complement Altern Med. 2018 Apr 5;18(1):123. (PMID: 29622019)
Oxid Med Cell Longev. 2015;2015:610813. (PMID: 25834699)
Eur J Obstet Gynecol Reprod Biol. 2006 Feb 1;124(2):168-72. (PMID: 16051414)
BMC Neurosci. 2017 Oct 27;18(1):76. (PMID: 29078760)
3 Biotech. 2018 Feb;8(2):124. (PMID: 29450114)
J Sep Sci. 2019 Mar;42(5):1088-1104. (PMID: 30663861)
فهرسة مساهمة: Keywords: Alzheimer’s diseases; acetylcholinesterase; butyrylcholinesterase; carlinoside; docking studies; lipid peroxidation
المشرفين على المادة: 0 (Antioxidants)
0 (Cholinesterase Inhibitors)
0 (Flavonoids)
0 (Plant Extracts)
EC 3.1.1.7 (Acetylcholinesterase)
EC 3.1.1.8 (Butyrylcholinesterase)
تواريخ الأحداث: Date Created: 20210501 Date Completed: 20211206 Latest Revision: 20220422
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8871626
DOI: 10.1080/13880209.2021.1913189
PMID: 33930998
قاعدة البيانات: MEDLINE
الوصف
تدمد:1744-5116
DOI:10.1080/13880209.2021.1913189