دورية أكاديمية
أعرض في EDS

Determination of nitrofuran metabolites in honey using a new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS.

التفاصيل البيبلوغرافية
العنوان: Determination of nitrofuran metabolites in honey using a new derivatization reagent, magnetic solid-phase extraction and LC-MS/MS.
المؤلفون: Melekhin AO; Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow, 119991, Russia; Central Scientific Methodological Veterinary Laboratory, Orangereynaya st., 23, 111622, Moscow, Russia., Tolmacheva VV; Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow, 119991, Russia., Shubina EG; Central Scientific Methodological Veterinary Laboratory, Orangereynaya st., 23, 111622, Moscow, Russia., Dmitrienko SG; Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow, 119991, Russia., Apyari VV; Analytical Chemistry Division, Department of Chemistry, Lomonosov Moscow State University, Leninskie Gory, 1/3, Moscow, 119991, Russia. Electronic address: apyari@mail.ru., Grudev AI; Central Scientific Methodological Veterinary Laboratory, Orangereynaya st., 23, 111622, Moscow, Russia.
المصدر: Talanta [Talanta] 2021 Aug 01; Vol. 230, pp. 122310. Date of Electronic Publication: 2021 Mar 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam : Elsevier
Original Publication: Oxford : Pergamon Press
مواضيع طبية MeSH: Honey*/analysis , Nitrofurans*/analysis, Chromatography, Liquid ; Indicators and Reagents ; Magnetic Phenomena ; Solid Phase Extraction ; Tandem Mass Spectrometry
مستخلص: In this study, 5-nitro-2-furaldehyde (5-NFA) was proposed as a new derivatizing agent for nitrofuran metabolites. It reacts with nitrofuran metabolites producing the parent nitrofurans (furazolidone, furaltadone, nitrofurantoin, and nitrofurazone). Magnetic hypercrosslinked polystyrene (HCP/Fe 3 O 4 ) was first used for magnetic solid phase extraction (MSPE) clean-up before the determination of nitrofuran metabolite derivatives in honey via LC-MS/MS. Main parameters affecting the derivatization and MSPE efficiency were investigated in detail and the optimal conditions were found. The method was validated using honey spiked with the four metabolites at 1, 2 and 200 μg kg -1 . Recoveries of >85% were achieved for the all analytes. The matrix calibration curve was fitted with the correlation coefficient (R 2 ) > 0.99 in the range of 1-200 μg kg -1 . Precision values expressed as relative standard deviation (RSD) were <12% and <15% for intra-day and inter-day precision, respectively. The limits of detection (LODs) for the nitrofuran metabolites were of 0.1-0.3 μg kg -1 and the limits of quantitation (LOQs) were of 0.3-1.0 μg kg -1 . The proposed LC-MS/MS method was applied to the analysis of real honey samples.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: 5-nitro-2-furaldehyde; Honey; LC–MS/MS; Magnetic hypercrosslinked polystyrene; Magnetic solid-phase extraction; Nitrofuran metabolites
المشرفين على المادة: 0 (Indicators and Reagents)
0 (Nitrofurans)
تواريخ الأحداث: Date Created: 20210503 Date Completed: 20210514 Latest Revision: 20210514
رمز التحديث: 20231215
DOI: 10.1016/j.talanta.2021.122310
PMID: 33934775
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-3573
DOI:10.1016/j.talanta.2021.122310