دورية أكاديمية
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Synthase-Selective Exploration of a Tunicate Microbiome by Activity-Guided Single-Cell Genomics.

التفاصيل البيبلوغرافية
العنوان: Synthase-Selective Exploration of a Tunicate Microbiome by Activity-Guided Single-Cell Genomics.
المؤلفون: Kim WE; Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, United States., Charov K; Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, United States., Džunková M; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Mail Stop: 91R183, 1 Cyclotron Road, Berkeley, California 94720, United States., Becraft ED; Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine 04544, United States.; University of North Alabama, Florence, Alabama 35632, United States., Brown J; Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine 04544, United States., Schulz F; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Mail Stop: 91R183, 1 Cyclotron Road, Berkeley, California 94720, United States., Woyke T; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Mail Stop: 91R183, 1 Cyclotron Road, Berkeley, California 94720, United States., La Clair JJ; Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, United States., Stepanauskas R; Bigelow Laboratory for Ocean Sciences, East Boothbay, Maine 04544, United States., Burkart MD; Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, California 92093-0358, United States.
المصدر: ACS chemical biology [ACS Chem Biol] 2021 May 21; Vol. 16 (5), pp. 813-819. Date of Electronic Publication: 2021 May 06.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101282906 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1554-8937 (Electronic) Linking ISSN: 15548929 NLM ISO Abbreviation: ACS Chem Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, D.C. : American Chemical Society, c2006-
مواضيع طبية MeSH: Genomics/*methods , Microbiota/*genetics , Multigene Family/*genetics , Peptide Synthases/*metabolism , Siderophores/*genetics, Animals ; Bacillus subtilis/genetics ; Carrier Proteins/chemistry ; Carrier Proteins/genetics ; Ciona intestinalis/metabolism ; Computational Biology ; Escherichia coli/genetics ; Flow Cytometry ; Phylogeny ; Polyketides/chemistry ; Secondary Metabolism ; Siderophores/chemistry ; Single-Cell Analysis
مستخلص: While thousands of environmental metagenomes have been mined for the presence of novel biosynthetic gene clusters, such computational predictions do not provide evidence of their in vivo biosynthetic functionality. Using fluorescent in situ enzyme assay targeting carrier proteins common to polyketide (PKS) and nonribosomal peptide synthetases (NRPS), we applied fluorescence-activated cell sorting to tunicate microbiome to enrich for microbes with active secondary metabolic capabilities. Single-cell genomics uncovered the genetic basis for a wide biosynthetic diversity in the enzyme-active cells and revealed a member of marine Oceanospirillales harboring a novel NRPS gene cluster with high similarity to phylogenetically distant marine and terrestrial bacteria. Interestingly, this synthase belongs to a larger class of siderophore biosynthetic gene clusters commonly associated with pestilence and disease. This demonstrates activity-guided single-cell genomics as a tool to guide novel biosynthetic discovery.
References: Acc Chem Res. 2017 Feb 21;50(2):366-375. (PMID: 28067047)
Front Microbiol. 2016 Dec 27;7:2092. (PMID: 28082961)
Ann Rev Mar Sci. 2017 Jan 3;9:231-255. (PMID: 27687974)
Trends Pharmacol Sci. 2020 Jan;41(1):13-26. (PMID: 31822352)
J Antibiot (Tokyo). 2008 Jan;61(1):11-7. (PMID: 18305354)
Nucleic Acids Res. 2017 Jul 3;45(W1):W72-W79. (PMID: 28460065)
Front Microbiol. 2019 Nov 05;10:2467. (PMID: 31749778)
Proteins. 2014 Oct;82(10):2691-702. (PMID: 24975514)
Biochemistry. 2012 Apr 17;51(15):3252-63. (PMID: 22452656)
Nat Commun. 2018 Dec 7;9(1):5253. (PMID: 30531862)
PLoS One. 2012;7(4):e35314. (PMID: 22536372)
Nature. 2019 Jul;571(7765):349-354. (PMID: 31292549)
J Microbiol. 2011 Apr;49(2):233-7. (PMID: 21538243)
Chem Commun (Camb). 2010 Nov 21;46(43):8151-3. (PMID: 20927429)
Mar Drugs. 2017 Jun 06;15(6):. (PMID: 28587290)
J Mol Biol. 1990 Oct 5;215(3):403-10. (PMID: 2231712)
Nucleic Acids Res. 2019 Jul 2;47(W1):W81-W87. (PMID: 31032519)
Genome Biol Evol. 2019 Nov 1;11(11):3144-3157. (PMID: 31621849)
Nat Commun. 2017 Jul 20;8(1):84. (PMID: 28729688)
Biochemistry. 2000 Dec 19;39(50):15522-30. (PMID: 11112538)
Arch Microbiol. 1993;160(5):333-7. (PMID: 8257279)
ACS Infect Dis. 2019 Sep 13;5(9):1518-1523. (PMID: 31317739)
Curr Opin Chem Biol. 2021 Feb;60:47-54. (PMID: 32853968)
Cell. 2019 Dec 12;179(7):1623-1635.e11. (PMID: 31835036)
ISME J. 2014 Feb;8(2):309-20. (PMID: 24048225)
FEMS Microbiol Rev. 2017 Jul 1;41(4):538-548. (PMID: 28591840)
Nat Prod Rep. 2014 Jan;31(1):61-108. (PMID: 24292120)
Int J Syst Evol Microbiol. 2015 Jun;65(Pt 6):1866-1870. (PMID: 25766110)
Methods Enzymol. 2009;458:181-217. (PMID: 19374984)
J Am Chem Soc. 2008 Oct 15;130(41):13630-8. (PMID: 18808123)
Nucleic Acids Res. 2020 Jan 8;48(D1):D422-D430. (PMID: 31665416)
Nucleic Acids Res. 2019 Jan 8;47(D1):D666-D677. (PMID: 30289528)
Nat Microbiol. 2019 Dec;4(12):2498-2510. (PMID: 31611640)
معلومات مُعتمدة: P30 NS047101 United States NS NINDS NIH HHS; R01 GM095970 United States GM NIGMS NIH HHS; R21 AI134037 United States AI NIAID NIH HHS
المشرفين على المادة: 0 (Carrier Proteins)
0 (Polyketides)
0 (Siderophores)
EC 6.3.2.- (Peptide Synthases)
EC 6.3.2.- (non-ribosomal peptide synthase)
تواريخ الأحداث: Date Created: 20210506 Date Completed: 20210824 Latest Revision: 20230130
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9884146
DOI: 10.1021/acschembio.1c00157
PMID: 33955744
قاعدة البيانات: MEDLINE
الوصف
تدمد:1554-8937
DOI:10.1021/acschembio.1c00157