دورية أكاديمية
DNA Gold Nanoparticle Motors Demonstrate Processive Motion with Bursts of Speed Up to 50 nm Per Second.
| العنوان: | DNA Gold Nanoparticle Motors Demonstrate Processive Motion with Bursts of Speed Up to 50 nm Per Second. |
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| المؤلفون: | Bazrafshan A; Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322 United States., Kyriazi ME; School of Physics and Astronomy, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO171BJ, U.K., Holt BA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30322 United States., Deng W; Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322 United States., Piranej S; Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322 United States., Su H; Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322 United States., Hu Y; Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322 United States., El-Sagheer AH; Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Road, Oxford, OX1 3TA, U.K.; Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez, 43721, Egypt., Brown T; Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Road, Oxford, OX1 3TA, U.K., Kwong GA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30322 United States., Kanaras AG; School of Physics and Astronomy, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, SO171BJ, U.K.; Institute for Life Sciences, University of Southampton, Southampton, SO171BJ, U.K., Salaita K; Department of Chemistry, Emory University, 1515 Dickey Drive, Atlanta, Georgia 30322 United States.; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30322 United States. |
| المصدر: | ACS nano [ACS Nano] 2021 May 25; Vol. 15 (5), pp. 8427-8438. Date of Electronic Publication: 2021 May 06. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101313589 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1936-086X (Electronic) Linking ISSN: 19360851 NLM ISO Abbreviation: ACS Nano Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington D.C. : American Chemical Society |
| مواضيع طبية MeSH: | Gold* , Metal Nanoparticles*, DNA ; Dyneins ; Motion |
| مستخلص: | Synthetic motors that consume chemical energy to produce mechanical work offer potential applications in many fields that span from computing to drug delivery and diagnostics. Among the various synthetic motors studied thus far, DNA-based machines offer the greatest programmability and have shown the ability to translocate micrometer-distances in an autonomous manner. DNA motors move by employing a burnt-bridge Brownian ratchet mechanism, where the DNA "legs" hybridize and then destroy complementary nucleic acids immobilized on a surface. We have previously shown that highly multivalent DNA motors that roll offer improved performance compared to bipedal walkers. Here, we use DNA-gold nanoparticle conjugates to investigate and enhance DNA nanomotor performance. Specifically, we tune structural parameters such as DNA leg density, leg span, and nanoparticle anisotropy as well as buffer conditions to enhance motor performance. Both modeling and experiments demonstrate that increasing DNA leg density boosts the speed and processivity of motors, whereas DNA leg span increases processivity and directionality. By taking advantage of label-free imaging of nanomotors, we also uncover Lévy-type motion where motors exhibit bursts of translocation that are punctuated with transient stalling. Dimerized particles also demonstrate more ballistic trajectories confirming a rolling mechanism. Our work shows the fundamental properties that control DNA motor performance and demonstrates optimized motors that can travel multiple micrometers within minutes with speeds of up to 50 nm/s. The performance of these nanoscale motors approaches that of motor proteins that travel at speeds of 100-1000 nm/s, and hence this work can be important in developing protocellular systems as well next generation sensors and diagnostics. |
| فهرسة مساهمة: | Keywords: burnt bridge Brownian ratchet; dynamic DNA nanotechnology; gold nanoparticle; spherical nucleic acids; synthetic DNA motors |
| المشرفين على المادة: | 7440-57-5 (Gold) 9007-49-2 (DNA) EC 3.6.4.2 (Dyneins) |
| تواريخ الأحداث: | Date Created: 20210506 Date Completed: 20210609 Latest Revision: 20210609 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/acsnano.0c10658 |
| PMID: | 33956424 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1936-086X |
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| DOI: | 10.1021/acsnano.0c10658 |