دورية أكاديمية
Dimethylacetamide in Rooster Semen Cryopreservation.
| العنوان: | Dimethylacetamide in Rooster Semen Cryopreservation. |
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| المؤلفون: | Gatti NLS; Pos-Graduacao em Veterinaria, Faculdade de Veterinaria, Universidade Federal de Rio Grande, Rio Grande, Brasil., Corcini CD; Departamento de Patologia Animal, Laboratorio de Reproducao Animal, Faculdade de Veterinaria; Universidade Federal de Rio Grande, Rio Grande, Brasil. antoniovarela@furg.br., Filho JS; Pos-Graduacao em Veterinaria, Faculdade de Veterinaria; Universidade Federal de Rio Grande, Rio Grande, Brasil., Soares SL; Pos-Graduacao em Biotecnologia, Centro de desenvolvinto tecnologico CDTEC / Biotecnologia; Universidade Federal de Rio Grande, Rio Grande, Brasil., Anciuti AN; Pos-Graduacao em Veterinaria, Faculdade de Veterinaria; Universidade Federal de Rio Grande, Rio Grande, Brasil., Barbosa RM; Pos-Graduacao em Veterinaria, Faculdade de Veterinaria; Universidade Federal de Rio Grande, Rio Grande, Brasil., Knabah NW; Pos-Graduacao em Veterinaria, Faculdade de Veterinaria; Universidade Federal de Rio Grande, Rio Grande, Brasil., Tavares AT; Instituto de Biologia, Departamento de Fisiologia e Farmacologia, Universidade Federal de Pelotas, Universidade Federal de Rio Grande, Rio Grande, Brasil., Bongalhardo DC; Reproducao Animal Comparada, Instituto de Ciencia Biologicas, Universidade Federal de Rio Grande, Rio Grande, Brasil., Varela Junior AS; Reproducao Animal Comparada, Instituto de Ciencia Biologicas, Universidade Federal de Rio Grande, Rio Grande, Brasil. |
| المصدر: | Cryo letters [Cryo Letters] 2021 Jan-Feb; Vol. 42 (1), pp. 39-43. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: CryoLetters Country of Publication: England NLM ID: 9891832 Publication Model: Print Cited Medium: Print ISSN: 0143-2044 (Print) Linking ISSN: 01432044 NLM ISO Abbreviation: Cryo Letters Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London, Eng. : CryoLetters Original Publication: [London : Royal Veterinary College, |
| مواضيع طبية MeSH: | Chickens*, Acetamides/*pharmacology , Cryopreservation/*veterinary , Semen Preservation/*veterinary, Animals ; Cryoprotective Agents/pharmacology ; Freezing ; Male ; Semen ; Semen Analysis ; Sperm Motility ; Spermatozoa |
| مستخلص: | Background: Sperm cryopreservation of cockerels is a major challenge, and so far there is no adequate information to enable commercial use of frozen semen. Objective: To test the toxicity of dimethylacetamide (DMA). Materials and Methods: DMA was added at 3%, 6%, 9% and 12% to the freezing diluent, and maintained for equilibration with the semen sample for 1 min, 3 min, 5 min, 7 min and 9 min prior to freezing. Thawed semen was evaluated for kinetic characteristics by computer-assisted semen analysis (CASA) and for structural and functional properties by flow cytometry (plasma membrane rupture, mitochondrial functionality and plasma membrane functionality). Results and Conclusion: The addition of 6% DMA for 3-min equilibration resulted in the highest total and progressive motility, 42.0% and 36.9%, respectively. The point of intersection between a good protection and low plasma membrane rupture was obtained with the addition of 6% of DMA for 3-min equilibration with the rooster semen. |
| المشرفين على المادة: | 0 (Acetamides) 0 (Cryoprotective Agents) JCV5VDB3HY (dimethylacetamide) |
| تواريخ الأحداث: | Date Created: 20210511 Date Completed: 20210726 Latest Revision: 20210726 |
| رمز التحديث: | 20231215 |
| PMID: | 33973991 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0143-2044 |
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