دورية أكاديمية
Conformational changes during the reaction cycle of plasma membrane Ca2+-ATPase in the autoinhibited and activated states.
| العنوان: | Conformational changes during the reaction cycle of plasma membrane Ca2+-ATPase in the autoinhibited and activated states. |
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| المؤلفون: | Saffioti NA; Instituto de Química y Fisicoquímica Biológicas Dr. Alejandro Paladini (IQUIFIB), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Junín 956, Ciudad Autónoma de Buenos Aires C1113AAD, Argentina., de Sautu M; Instituto de Química y Fisicoquímica Biológicas Dr. Alejandro Paladini (IQUIFIB), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Junín 956, Ciudad Autónoma de Buenos Aires C1113AAD, Argentina., Riesco AS; Instituto de Química y Fisicoquímica Biológicas Dr. Alejandro Paladini (IQUIFIB), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Junín 956, Ciudad Autónoma de Buenos Aires C1113AAD, Argentina., Ferreira-Gomes MS; Instituto de Química y Fisicoquímica Biológicas Dr. Alejandro Paladini (IQUIFIB), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Junín 956, Ciudad Autónoma de Buenos Aires C1113AAD, Argentina., Rossi JPFC; Instituto de Química y Fisicoquímica Biológicas Dr. Alejandro Paladini (IQUIFIB), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Junín 956, Ciudad Autónoma de Buenos Aires C1113AAD, Argentina., Mangialavori IC; Instituto de Química y Fisicoquímica Biológicas Dr. Alejandro Paladini (IQUIFIB), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Junín 956, Ciudad Autónoma de Buenos Aires C1113AAD, Argentina. |
| المصدر: | The Biochemical journal [Biochem J] 2021 May 28; Vol. 478 (10), pp. 2019-2034. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published by Portland Press on behalf of the Biochemical Society Country of Publication: England NLM ID: 2984726R Publication Model: Print Cited Medium: Internet ISSN: 1470-8728 (Electronic) Linking ISSN: 02646021 NLM ISO Abbreviation: Biochem J Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London, UK : Published by Portland Press on behalf of the Biochemical Society |
| مواضيع طبية MeSH: | Adenosine Triphosphate/*metabolism , Calcium/*metabolism , Calmodulin/*metabolism , Cell Membrane/*metabolism , Plasma Membrane Calcium-Transporting ATPases/*chemistry , Plasma Membrane Calcium-Transporting ATPases/*metabolism, Binding Sites ; Calmodulin/genetics ; Humans ; Kinetics ; Phosphorylation ; Protein Binding ; Protein Conformation |
| مستخلص: | Plasma membrane Ca2+-ATPase (PMCA) transports Ca2+ by a reaction cycle including phosphorylated intermediates. Calmodulin binding to the C-terminal tail disrupts autoinhibitory interactions, activating the pump. To assess the conformational changes during the reaction cycle, we studied the structure of different PMCA states using a fluorescent probe, hydrophobic photolabeling, controlled proteolysis and Ca2+-ATPase activity. Our results show that calmodulin binds to E2P-like states, and during dephosphorylation, the hydrophobicity in the nucleotide-binding pocket decreases and the Ca2+ binding site becomes inaccessible to the extracellular medium. Autoinhibitory interactions are disrupted in E1Ca and in the E2P ground state whereas they are stabilized in the E2·Pi product state. Finally, we propose a model that describes the conformational changes during the Ca2+ transport of PMCA. (© 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.) |
| فهرسة مساهمة: | Keywords: E2P-like states; P-ATPases; autoinhibitory interaction; hydrophobic photolabeling; nucleitde-binding pocket; proteinase K cleavage |
| المشرفين على المادة: | 0 (ATP2B1 protein, human) 0 (Calmodulin) 8L70Q75FXE (Adenosine Triphosphate) EC 3.6.3.8 (Plasma Membrane Calcium-Transporting ATPases) SY7Q814VUP (Calcium) |
| تواريخ الأحداث: | Date Created: 20210511 Date Completed: 20211122 Latest Revision: 20211122 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1042/BCJ20210036 |
| PMID: | 33974040 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1470-8728 |
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| DOI: | 10.1042/BCJ20210036 |