دورية أكاديمية
أعرض في EDS

Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing.

التفاصيل البيبلوغرافية
العنوان: Quantitative profiling of pseudouridylation dynamics in native RNAs with nanopore sequencing.
المؤلفون: Begik O; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.; Garvan Institute of Medical Research, Darlinghurst, NSW, Australia.; UNSW Sydney, Kensington, NSW, Australia., Lucas MC; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.; Universitat Pompeu Fabra (UPF), Barcelona, Spain., Pryszcz LP; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.; International Institute of Molecular and Cell Biology, Warsaw, Poland., Ramirez JM; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain., Medina R; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain., Milenkovic I; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.; Universitat Pompeu Fabra (UPF), Barcelona, Spain., Cruciani S; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.; Universitat Pompeu Fabra (UPF), Barcelona, Spain., Liu H; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain., Vieira HGS; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain., Sas-Chen A; Weizmann Institute of Science, Rehovot, Israel., Mattick JS; UNSW Sydney, Kensington, NSW, Australia., Schwartz S; Weizmann Institute of Science, Rehovot, Israel., Novoa EM; Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain. eva.novoa@crg.eu.; Universitat Pompeu Fabra (UPF), Barcelona, Spain. eva.novoa@crg.eu.
المصدر: Nature biotechnology [Nat Biotechnol] 2021 Oct; Vol. 39 (10), pp. 1278-1291. Date of Electronic Publication: 2021 May 13.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature America Publishing Country of Publication: United States NLM ID: 9604648 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1546-1696 (Electronic) Linking ISSN: 10870156 NLM ISO Abbreviation: Nat Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Publication: New York Ny : Nature America Publishing
Original Publication: New York, NY : Nature Pub. Co., [1996-
مواضيع طبية MeSH: Nanopore Sequencing/*methods , Pseudouridine/*metabolism , RNA/*metabolism , Sequence Analysis, RNA/*methods, Algorithms ; Gene Expression Profiling ; Intramolecular Transferases/metabolism ; Mitochondria/genetics ; Pseudouridine/genetics ; RNA/genetics ; RNA Processing, Post-Transcriptional/genetics ; RNA, Fungal/genetics ; RNA, Fungal/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; RNA, Ribosomal/genetics ; RNA, Ribosomal/metabolism ; Saccharomyces cerevisiae/genetics ; Software ; Stress, Physiological/genetics
مستخلص: Nanopore RNA sequencing shows promise as a method for discriminating and identifying different RNA modifications in native RNA. Expanding on the ability of nanopore sequencing to detect N 6 -methyladenosine, we show that other modifications, in particular pseudouridine (Ψ) and 2'-O-methylation (Nm), also result in characteristic base-calling 'error' signatures in the nanopore data. Focusing on Ψ modification sites, we detected known and uncovered previously unreported Ψ sites in mRNAs, non-coding RNAs and rRNAs, including a Pus4-dependent Ψ modification in yeast mitochondrial rRNA. To explore the dynamics of pseudouridylation, we treated yeast cells with oxidative, cold and heat stresses and detected heat-sensitive Ψ-modified sites in small nuclear RNAs, small nucleolar RNAs and mRNAs. Finally, we developed a software, nanoRMS, that estimates per-site modification stoichiometries by identifying single-molecule reads with altered current intensity and trace profiles. This work demonstrates that Nm and Ψ RNA modifications can be detected in cellular RNAs and that their modification stoichiometry can be quantified by nanopore sequencing of native RNA.
(© 2021. The Author(s), under exclusive licence to Springer Nature America, Inc.)
التعليقات: Comment in: Nat Methods. 2021 Jul;18(7):711. (PMID: 34239099)
References: Dominissini, D. et al. Topology of the human and mouse m 6 A RNA methylomes revealed by m 6 A-seq. Nature 485, 201–206 (2012). (PMID: 10.1038/nature1111222575960)
Meyer, K. D. et al. Comprehensive analysis of mRNA methylation reveals enrichment in 3′ UTRs and near stop codons. Cell 149, 1635–1646 (2012). (PMID: 22608085338339610.1016/j.cell.2012.05.003)
Carlile, T. M. et al. Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells. Nature 515, 143–146 (2014). (PMID: 25192136422464210.1038/nature13802)
Schwartz, S. et al. Transcriptome-wide mapping reveals widespread dynamic-regulated pseudouridylation of ncRNA and mRNA. Cell 159, 148–162 (2014). (PMID: 25219674418011810.1016/j.cell.2014.08.028)
Lovejoy, A. F., Riordan, D. P. & Brown, P. O. Transcriptome-wide mapping of pseudouridines: pseudouridine synthases modify specific mRNAs in S. cerevisiae. PLoS ONE 9, e110799 (2014). (PMID: 25353621421299310.1371/journal.pone.0110799)
Li, X. et al. Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome. Nat. Chem. Biol. 11, 592–597 (2015). (PMID: 2607552110.1038/nchembio.1836)
Hussain, S., Aleksic, J., Blanco, S., Dietmann, S. & Frye, M. Characterizing 5-methylcytosine in the mammalian epitranscriptome. Genome Biol. 14, 215 (2013). (PMID: 24286375405377010.1186/gb4143)
Huang, T., Chen, W., Liu, J., Gu, N. & Zhang, R. Genome-wide identification of mRNA 5-methylcytosine in mammals. Nat. Struct. Mol. Biol. 26, 380–388 (2019). (PMID: 3106152410.1038/s41594-019-0218-x)
Delatte, B. et al. RNA biochemistry. Transcriptome-wide distribution and function of RNA hydroxymethylcytosine. Science 351, 282–285 (2016). (PMID: 2681638010.1126/science.aac5253)
Safra, M. et al. The m 1 A landscape on cytosolic and mitochondrial mRNA at single-base resolution. Nature 551, 251–255 (2017). (PMID: 2907229710.1038/nature24456)
Li, X. et al. Base-resolution mapping reveals distinct m 1 A methylome in nuclear- and mitochondrial-encoded transcripts. Mol. Cell 68, 993–1005 (2017). (PMID: 29107537572268610.1016/j.molcel.2017.10.019)
Marchand, V. et al. AlkAniline-Seq: profiling of m 7 G and m 3 C RNA modifications at single nucleotide resolution. Angew. Chem. Int. Ed. 57, 16785–16790 (2018). (PMID: 10.1002/anie.201810946)
Arango, D. et al. Acetylation of cytidine in mrna promotes translation efficiency. Cell 175, 1872–1886 (2018). (PMID: 30449621629523310.1016/j.cell.2018.10.030)
Sas-Chen, A. et al. Dynamic RNA acetylation revealed by quantitative cross-evolutionary mapping. Nature 583, 638–643 (2020).
Zhang, L.-S. et al. Transcriptome-wide mapping of internal N 7 -methylguanosine methylome in mammalian mRNA. Mol. Cell 74, 1304–1316.e8 (2019). (PMID: 31031084658848310.1016/j.molcel.2019.03.036)
Pandolfini, L. et al. METTL1 promotes let-7 microRNA processing via m 7 G methylation. Mol. Cell 74, 1278–1290 (2019). (PMID: 31031083659100210.1016/j.molcel.2019.03.040)
Delaunay, S. & Frye, M. RNA modifications regulating cell fate in cancer. Nat. Cell Biol. 21, 552–559 (2019). (PMID: 3104877010.1038/s41556-019-0319-0)
Haussmann, I. U. et al. m 6 A potentiates Sxl alternative pre-mRNA splicing for robust Drosophila sex determination. Nature 540, 301–304 (2016). (PMID: 2791908110.1038/nature20577)
Vu, L. P. et al. The N 6 -methyladenosine (m 6 A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells. Nat. Med. 23, 1369–1376 (2017). (PMID: 28920958567753610.1038/nm.4416)
Novoa, E. M., Mason, C. E. & Mattick, J. S. Charting the unknown epitranscriptome. Nat. Rev. Mol. Cell Biol. 18, 339–340 (2017). (PMID: 2848869910.1038/nrm.2017.49)
Anreiter, I., Mir, Q., Simpson, J. T., Janga, S. C. & Soller, M. New twists in detecting mRNA modification dynamics. Trends Biotechnol. 39, 72–89 (2020).
Li, X., Xiong, X. & Yi, C. Epitranscriptome sequencing technologies: decoding RNA modifications. Nat. Methods 14, 23–31 (2016). (PMID: 2803262210.1038/nmeth.4110)
Motorin, Y. & Helm, M. Methods for RNA modification mapping using deep sequencing: established and new emerging technologies. Genes 10, 35 (2019). (PMID: 635670710.3390/genes10010035)
Grozhik, A. V. et al. Antibody cross-reactivity accounts for widespread appearance of m 1 A in 5′UTRs. Nat. Commun. 10, 1–13 (2019). (PMID: 10.1038/s41467-019-13146-w)
Lahens, N. F. et al. IVT-seq reveals extreme bias in RNA sequencing. Genome Biol. 15, R86 (2014). (PMID: 24981968419782610.1186/gb-2014-15-6-r86)
Garalde, D. R. et al. Highly parallel direct RNA sequencing on an array of nanopores. Nat. Methods 15, 201–206 (2018). (PMID: 2933437910.1038/nmeth.4577)
Jonkhout, N. et al. The RNA modification landscape in human disease. RNA 23, 1754–1769 (2017). (PMID: 28855326568899710.1261/rna.063503.117)
Leger, A., Amaral, P. P., Pandolfini, L. & Capitanchik, C. RNA modifications detection by comparative Nanopore direct RNA sequencing. Preprint at bioRxiv https://doi.org/10.1101/843136 (2019).
Liu, H. et al. Accurate detection of m 6 A RNA modifications in native RNA sequences. Nat. Commun. 10, 4079 (2019). (PMID: 31501426673400310.1038/s41467-019-11713-9)
Parker, M. T. et al. Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m 6 A modification. eLife 9, e49658 (2020). (PMID: 31931956695999710.7554/eLife.49658)
Price, A. M. et al. Direct RNA sequencing reveals m 6 A modifications on adenovirus RNA are necessary for efficient splicing. Nat. Commun. 11, 6016 (2020).
Jenjaroenpun, P. et al. Decoding the epitranscriptional landscape from native RNA sequences. Nucleic Acids Res. 49, e7 (2020). (PMID: 782625410.1093/nar/gkaa620)
Lorenz, D. A., Sathe, S., Einstein, J. M. & Yeo, G. W. Direct RNA sequencing enables m 6 A detection in endogenous transcript isoforms at base-specific resolution. RNA 26, 19–28 (2020). (PMID: 31624092691313210.1261/rna.072785.119)
Pratanwanich, P. N. et al. Detection of differential RNA modifications from direct RNA sequencing of human cell lines. Preprint at bioRxiv https://doi.org/10.1101/2020.06.18.160010 (2020).
Jack, K. et al. rRNA pseudouridylation defects affect ribosomal ligand binding and translational fidelity from yeast to human cells. Mol. Cell 44, 660–666 (2011). (PMID: 22099312322287310.1016/j.molcel.2011.09.017)
Yoon, A. et al. Impaired control of IRES-mediated translation in X-linked dyskeratosis congenita. Science 312, 902–906 (2006). (PMID: 1669086410.1126/science.1123835)
Bellodi, C. et al. Loss of function of the tumor suppressor DKC1 perturbs p27 translation control and contributes to pituitary tumorigenesis. Cancer Res. 70, 6026–6035 (2010). (PMID: 20587522291386410.1158/0008-5472.CAN-09-4730)
Wu, G., Xiao, M., Yang, C. & Yu, Y.-T. U2 snRNA is inducibly pseudouridylated at novel sites by Pus7p and snR81 RNP. EMBO J. 30, 79–89 (2011). (PMID: 2113190910.1038/emboj.2010.316)
Taoka, M., Nobe, Y., Hori, M. & Takeuchi, A. A mass spectrometry-based method for comprehensive quantitative determination of post-transcriptional RNA modifications: the complete chemical structure of Schizosaccharomyces pombe ribosomal RNAs. Nucleic Acids Res. 43, e115 (2015).
Basu, A. et al. Requirement of rRNA methylation for 80S ribosome assembly on a cohort of cellular internal ribosome entry sites. Mol. Cell. Biol. 31, 4482–4499 (2011). (PMID: 21930789320926110.1128/MCB.05804-11)
Marcel, V. et al. p53 acts as a safeguard of translational control by regulating fibrillarin and rRNA methylation in cancer. Cancer Cell 24, 318–330 (2013). (PMID: 24029231710627710.1016/j.ccr.2013.08.013)
Belin, S. et al. Dysregulation of ribosome biogenesis and translational capacity is associated with tumor progression of human breast cancer cells. PLoS ONE 4, e7147 (2009). (PMID: 19779612274499810.1371/journal.pone.0007147)
Buchhaupt, M. et al. Partial methylation at Am100 in 18S rRNA of baker’s yeast reveals ribosome heterogeneity on the level of eukaryotic rRNA modification. PLoS ONE 9, e89640 (2014). (PMID: 24586927393849310.1371/journal.pone.0089640)
Chen, H. et al. METTL5, an 18S rRNA-specific m 6 A methyltransferase, modulates expression of stress response genes. Preprint at bioRxiv https://doi.org/10.1101/2020.04.27.064162 (2020).
Li, H. Minimap2: pairwise alignment for nucleotide sequences. Bioinformatics 34, 3094–3100 (2018). (PMID: 29750242613799610.1093/bioinformatics/bty191)
Sović, I. et al. Fast and sensitive mapping of nanopore sequencing reads with GraphMap. Nat. Commun. 7, 11307 (2016). (PMID: 27079541483554910.1038/ncomms11307)
Liu, Q. et al. Detection of DNA base modifications by deep recurrent neural network on Oxford Nanopore sequencing data. Nat. Commun. 10, 2449 (2019). (PMID: 31164644654772110.1038/s41467-019-10168-2)
McIntyre, A. B. R. et al. Single-molecule sequencing detection of N 6 -methyladenine in microbial reference materials. Nat. Commun. 10, 579 (2019). (PMID: 30718479636208810.1038/s41467-019-08289-9)
De Coster, W., Stovner, E. B. & Strazisar, M. Methplotlib: analysis of modified nucleotides from nanopore sequencing. Bioinformatics 36, 3236–3238 (2020). (PMID: 32053166721403810.1093/bioinformatics/btaa093)
Stoiber, M. et al. De novo identification of DNA modifications enabled by genome-guided nanopore signal processing. Preprint at bioRxiv https://doi.org/10.1101/094672 (2017).
Taoka, M. et al. The complete chemical structure of Saccharomyces cerevisiae rRNA: partial pseudouridylation of U2345 in 25S rRNA by snoRNA snR9. Nucleic Acids Res. 44, 8951–8961 (2016). (PMID: 27325748506296910.1093/nar/gkw564)
Pintard, L., Bujnicki, J. M., Lapeyre, B. & Bonnerot, C. MRM2 encodes a novel yeast mitochondrial 21S rRNA methyltransferase. EMBO J. 21, 1139–1147 (2002). (PMID: 1186754212588810.1093/emboj/21.5.1139)
Sharma, S. & Lafontaine, D. L. J. ‘View from a bridge’: a new perspective on eukaryotic rRNA base modification. Trends Biochem. Sci 40, 560–575 (2015). (PMID: 2641059710.1016/j.tibs.2015.07.008)
Taoka, M. et al. Landscape of the complete RNA chemical modifications in the human 80S ribosome. Nucleic Acids Res. 46, 9289–9298 (2018). (PMID: 30202881618216010.1093/nar/gky811)
Fischer, N. et al. Structure of the E. coli ribosome–EF-Tu complex at <3 Å resolution by C s -corrected cryo-EM. Nature 520, 567–570 (2015). (PMID: 2570780210.1038/nature14275)
Sergeeva, O. V., Bogdanov, A. A. & Sergiev, P. V. What do we know about ribosomal RNA methylation in Escherichia coli? Biochimie 117, 110–118 (2015). (PMID: 2551142310.1016/j.biochi.2014.11.019)
Sloan, K. E. et al. Tuning the ribosome: the influence of rRNA modification on eukaryotic ribosome biogenesis and function. RNA Biol. 14, 1138–1152 (2017). (PMID: 2791118810.1080/15476286.2016.1259781)
Hebras, J., Krogh, N., Marty, V., Nielsen, H. & Cavaillé, J. Developmental changes of rRNA ribose methylations in the mouse. RNA Biol. 17, 150–164 (2020).
Higa-Nakamine, S. et al. Loss of ribosomal RNA modification causes developmental defects in zebrafish. Nucleic Acids Res. 40, 391–398 (2012). (PMID: 2190840210.1093/nar/gkr700)
Sahoo, T. et al. Prader–Willi phenotype caused by paternal deficiency for the HBII-85 C/D box small nucleolar RNA cluster. Nat. Genet. 40, 719–721 (2008). (PMID: 18500341270519710.1038/ng.158)
Heiss, N. S. et al. X-linked dyskeratosis congenita is caused by mutations in a highly conserved gene with putative nucleolar functions. Nat. Genet. 19, 32–38 (1998). (PMID: 959028510.1038/ng0598-32)
Knight, S. W. et al. X-linked dyskeratosis congenita is predominantly caused by missense mutations in the DKC1 gene. Am. J. Hum. Genet. 65, 50–58 (1999). (PMID: 10364516137807410.1086/302446)
Liao, J. et al. Small nucleolar RNA signatures as biomarkers for non-small-cell lung cancer. Mol. Cancer 9, 198 (2010). (PMID: 20663213291945010.1186/1476-4598-9-198)
Mei, Y.-P. et al. Small nucleolar RNA 42 acts as an oncogene in lung tumorigenesis. Oncogene 31, 2794–2804 (2012). (PMID: 2198694610.1038/onc.2011.449)
Bortolin-Cavaille, M.-L., Bortolin-Cavaille, M. & Cavaille, J. The SNORD115 (H/MBII-52) and SNORD116 (H/MBII-85) gene clusters at the imprinted Prader–Willi locus generate canonical box C/D snoRNAs. Nucleic Acids Res. 40, 6800–6807 (2012). (PMID: 22495932341313010.1093/nar/gks321)
Erales, J. et al. Evidence for rRNA 2′-O-methylation plasticity: control of intrinsic translational capabilities of human ribosomes. Proc. Natl Acad. Sci. USA 114, 12934–12939 (2017). (PMID: 29158377572425510.1073/pnas.1707674114)
Krogh, N. et al. Profiling of 2′-O-Me in human rRNA reveals a subset of fractionally modified positions and provides evidence for ribosome heterogeneity. Nucleic Acids Res. 44, 7884–7895 (2016). (PMID: 27257078502748210.1093/nar/gkw482)
Birkedal, U. et al. Profiling of ribose methylations in RNA by high-throughput sequencing. Angew. Chem. Int. Ed. Engl. 54, 451–455 (2015). (PMID: 25417815)
Natchiar, S. K., Myasnikov, A. G., Kratzat, H., Hazemann, I. & Klaholz, B. P. Visualization of chemical modifications in the human 80S ribosome structure. Nature 551, 472–477 (2017). (PMID: 2914381810.1038/nature24482)
van der Feltz, C., DeHaven, A. C. & Hoskins, A. A. Stress-induced pseudouridylation alters the structural equilibrium of yeast U2 snRNA stem II. J. Mol. Biol. 430, 524–536 (2018). (PMID: 2907948210.1016/j.jmb.2017.10.021)
Frye, M., Harada, B. T., Behm, M. & He, C. RNA modifications modulate gene expression during development. Science 361, 1346–1349 (2018). (PMID: 30262497643639010.1126/science.aau1646)
Roundtree, I. A., Evans, M. E., Pan, T. & He, C. Dynamic RNA modifications in gene expression regulation. Cell 169, 1187–1200 (2017). (PMID: 28622506565724710.1016/j.cell.2017.05.045)
Li, S. & Mason, C. E. The pivotal regulatory landscape of RNA modifications. Annu. Rev. Genomics Hum. Genet. 15, 127–150 (2014). (PMID: 2489803910.1146/annurev-genom-090413-025405)
Wang, X. et al. LARP7-mediated U6 snRNA modification ensures splicing fidelity and spermatogenesis in mice. Mol. Cell 77, 999–1013 (2020). (PMID: 3201789610.1016/j.molcel.2020.01.002)
Louloupi, A., Ntini, E., Conrad, T. & Ørom, U. A. V. Transient N-6-methyladenosine transcriptome sequencing reveals a regulatory role of m6A in splicing efficiency. Cell Rep. 23, 3429–3437 (2018). (PMID: 2992498710.1016/j.celrep.2018.05.077)
Zhou, K. I. et al. Regulation of co-transcriptional pre-mRNA splicing by m 6 A through the low-complexity protein hnRNPG. Mol. Cell 76, 70–81 (2019). (PMID: 31445886677802910.1016/j.molcel.2019.07.005)
Lee, Y., Choe, J., Park, O. H. & Kim, Y. K. Molecular mechanisms driving mRNA degradation by m 6 A modification. Trends Genet. 36, 177–188 (2020). (PMID: 3196450910.1016/j.tig.2019.12.007)
Guo, M., Liu, X., Zheng, X., Huang, Y. & Chen, X. m 6 A RNA modification determines cell fate by regulating mRNA degradation. Cell. Reprogram. 19, 225–231 (2017). (PMID: 2868266910.1089/cell.2016.0041)
Geula, S. et al. m 6 A mRNA methylation facilitates resolution of naïve pluripotency toward differentiation. Science 347, 1002–1006 (2015). (PMID: 2556911110.1126/science.1261417)
Weng, H. et al. METTL14 inhibits hematopoietic stem/progenitor differentiation and promotes leukemogenesis via mRNA m 6 A modification. Cell Stem Cell 22, 191–205 (2018). (PMID: 2929061710.1016/j.stem.2017.11.016)
Lence, T. et al. m 6 A modulates neuronal functions and sex determination in Drosophila. Nature 540, 242–247 (2016). (PMID: 2791907710.1038/nature20568)
Kan, L. et al. The m 6 A pathway facilitates sex determination in Drosophila. Nat. Commun. 8, 15737 (2017). (PMID: 28675155550088910.1038/ncomms15737)
Schaefer, M., Kapoor, U. & Jantsch, M. F. Understanding RNA modifications: the promises and technological bottlenecks of the ‘epitranscriptome’. Open Biol. 7, 170077 (2017). (PMID: 28566301545154810.1098/rsob.170077)
Depledge, D. P. et al. Direct RNA sequencing on nanopore arrays redefines the transcriptional complexity of a viral pathogen. Nat. Commun. 10, 754 (2019). (PMID: 30765700637612610.1038/s41467-019-08734-9)
Roach, N. P. et al. The full-length transcriptome of C. elegans using direct RNA sequencing. Genome Res. 30, 299–312 (2020). (PMID: 32024661705052010.1101/gr.251314.119)
Workman, R. E. et al. Nanopore native RNA sequencing of a human poly(A) transcriptome. Nat. Methods 16, 1297–1305 (2019).
Kim, D. et al. The architecture of SARS-CoV-2 transcriptome. Cell 181, 914–921 (2020). (PMID: 32330414717950110.1016/j.cell.2020.04.011)
Parker, S. et al. A resource for functional profiling of noncoding RNA in the yeast Saccharomyces cerevisiae. RNA 23, 1166–1171 (2017). (PMID: 28468764551306110.1261/rna.061564.117)
Smith, M. A. et al. Molecular barcoding of native RNAs using nanopore sequencing and deep learning. Genome Res. 30, 1345–1353 (2020). (PMID: 32907883754514610.1101/gr.260836.120)
Li, H. et al. The sequence Alignment/Map format and SAMtools. Bioinformatics 25, 2078–2079 (2009). (PMID: 19505943272300210.1093/bioinformatics/btp352)
Robinson, J. T. et al. Integrative genomics viewer. Nat. Biotechnol. 29, 24–26 (2011). (PMID: 21221095334618210.1038/nbt.1754)
Loman, N. J., Quick, J. & Simpson, J. T. A complete bacterial genome assembled de novo using only nanopore sequencing data. Nat. Methods 12, 733–735 (2015). (PMID: 2607642610.1038/nmeth.3444)
المشرفين على المادة: 0 (RNA, Fungal)
0 (RNA, Messenger)
0 (RNA, Ribosomal)
1445-07-4 (Pseudouridine)
63231-63-0 (RNA)
EC 5.4.- (Intramolecular Transferases)
EC 5.4.99.12 (tRNA-pseudouridine synthase I)
تواريخ الأحداث: Date Created: 20210514 Date Completed: 20211116 Latest Revision: 20220630
رمز التحديث: 20231215
DOI: 10.1038/s41587-021-00915-6
PMID: 33986546
قاعدة البيانات: MEDLINE
الوصف
تدمد:1546-1696
DOI:10.1038/s41587-021-00915-6