دورية أكاديمية
Photodynamic Therapy With Propolis: Antibacterial Effects on Staphylococcus aureus, Streptococcus mutans and Escherichia coli Analysed by Atomic Force Microscopy.
| العنوان: | Photodynamic Therapy With Propolis: Antibacterial Effects on Staphylococcus aureus, Streptococcus mutans and Escherichia coli Analysed by Atomic Force Microscopy. |
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| المؤلفون: | Fonseca AMV; Undergraduate Medical Student at the Faculty of Human, Exact and Health Sciences of Piauí of the Institute of Higher Education of Vale do Parnaíba, Parnaíba, PI, Brazil., Sampaio GHL; Undergraduate Medical Student at the Faculty of Human, Exact and Health Sciences of Piauí of the Institute of Higher Education of Vale do Parnaíba, Parnaíba, PI, Brazil., Araujo WP; Undergraduate Medical Student at the Faculty of Human, Exact and Health Sciences of Piauí of the Institute of Higher Education of Vale do Parnaíba, Parnaíba, PI, Brazil., da Silva RE; Biodiversity and Biotechnology Research Center (BIOTEC), Federal University of Piauí. Parnaíba, PI, Brazil., Ribeiro FOS; Biodiversity and Biotechnology Research Center (BIOTEC), Federal University of Piauí. Parnaíba, PI, Brazil., Brito MDP; Biodiversity and Biotechnology Research Center (BIOTEC), Federal University of Piauí. Parnaíba, PI, Brazil., Sousa FBM; Biodiversity and Biotechnology Research Center (BIOTEC), Federal University of Piauí. Parnaíba, PI, Brazil., Torres AA; Undergraduate Medical Student at the Federal University of Piauí, Parnaíba, PI, Brazil., Araújo AR; Biodiversity and Biotechnology Research Center (BIOTEC), Federal University of Piauí. Parnaíba, PI, Brazil., Pinto ASB; Medical Professor at the Faculty of Human, Exact and Health Sciences of Piauí of the Institute of Higher Education of Vale do Parnaíba, Parnaíba, PI, Brazil. |
| المصدر: | Journal of lasers in medical sciences [J Lasers Med Sci] 2020 Fall; Vol. 11 (Suppl 1), pp. S107-S112. Date of Electronic Publication: 2020 Dec 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Laser Application in Medical Sciences Research Center Country of Publication: Iran NLM ID: 101580415 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2008-9783 (Print) Linking ISSN: 20089783 NLM ISO Abbreviation: J Lasers Med Sci Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Tehran, Iran : Laser Application in Medical Sciences Research Center |
| مستخلص: | Introduction: Photodynamic therapy (PDT) is a process that uses a light source (e.g. laser), oxygen molecules and a photosensitizing agent. PDT aims to act against pathogens, including those resistant to antimicrobials. The association of PDT with natural drugs, such as Propolis, has not been widely studied. Methods: Therefore, this study aimed to evaluate the antimicrobial effect of PDT in vitro by using Propolis as a photosensitizing agent. For this purpose, the dry Propolis extract was used as a photosensitizer and a low-power laser (Photon Laser III model) was irradiated onto the microwells for 90 seconds. Gram-positive and Gram-negative bacterial strains were used in the tests at a concentration of 5 × 10 5 CFU/mL. Initially, the antibacterial activity of the photosensitizers without laser action was determined by using a serial microdilution method before the experiment with a laser. After the incubation of the plates in a bacteriological oven, resazurin (0.1%) was added and the minimum inhibitory concentration (MIC) was determined. Alterations in the morphology of the bacteria were analysed by using atomic force microscopy (AFM). Results: Bacteria were sensitive to Propolis with MICs ranging from 13.75 to 0.85 mg/mL, but no susceptibility was observed for methylene blue without laser application. A change was observed for MIC values of Propolis against Staphylococcus aureus after irradiation, which decreased from 1.71 mg/mL to 0.85 mg/mL. However, this behaviour was not observed in Escherichia coli , the only gram-negative strain used. In addition, AFM images revealed alterations in the size of one of the bacteria tested. Conclusion: The Propolis is more active against gram-positive bacteria and PDT improved its activity against one of the strains tested. (Copyright © 2020 J Lasers Med Sci.) |
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| فهرسة مساهمة: | Keywords: Antimicrobial activity; Photodynamic therapy; Propolis |
| تواريخ الأحداث: | Date Created: 20210517 Latest Revision: 20210518 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC7956024 |
| DOI: | 10.34172/jlms.2020.S17 |
| PMID: | 33995978 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2008-9783 |
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| DOI: | 10.34172/jlms.2020.S17 |