دورية أكاديمية
أعرض في EDS

Modeling the role for nuclear import dynamics in the early embryonic cell cycle.

التفاصيل البيبلوغرافية
العنوان: Modeling the role for nuclear import dynamics in the early embryonic cell cycle.
المؤلفون: Shindo Y; Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire. Electronic address: yuki.shindo@dartmouth.edu., Amodeo AA; Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire. Electronic address: amanda.a.amodeo@dartmouth.edu.
المصدر: Biophysical journal [Biophys J] 2021 Oct 05; Vol. 120 (19), pp. 4277-4286. Date of Electronic Publication: 2021 May 20.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 0370626 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1542-0086 (Electronic) Linking ISSN: 00063495 NLM ISO Abbreviation: Biophys J Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge, MA : Cell Press
Original Publication: New York, Published by Rockefeller University Press [etc.] for the Biophysical Society.
مواضيع طبية MeSH: Blastula* , Embryo, Nonmammalian*/metabolism, Active Transport, Cell Nucleus ; Animals ; Cell Cycle ; Cell Division ; Cell Nucleus/metabolism
مستخلص: Nuclear composition determines nuclear function. The early embryos of many species begin life with large pools of maternally provided components that become rapidly imported into an increasing number of nuclei as the cells undergo repeated cleavage divisions. Because early cell cycles are too fast for nuclei to achieve steady-state nucleocytoplasmic partitioning, the composition of cleavage stage nuclei is likely dominated by nuclear import. The end of the rapid cleavage stage and onset of major zygotic transcription, known as the mid-blastula transition (MBT), is controlled by the ratio of nuclei/cytoplasm, indicating that changes in nuclear composition likely mediate MBT timing. Here, we explore how different nuclear import regimes can affect protein accumulation in the nucleus in the early Drosophila embryo. We find that nuclear import differs dramatically for a general nuclear cargo (NLS (nuclear localization signal)-mRFP) and a proposed MBT regulator (histone H3). We show that nuclear import rates of NLS-mRFP in a given nucleus remain relatively unchanged throughout the cleavage cycles, whereas those of H3 halve with each cycle. We model these two distinct modes of nuclear import as "nucleus-limited" and "import-limited" and examine how the two different modes can contribute to different protein accumulation dynamics. Finally, we incorporate these distinct modes of nuclear import into a model for cell-cycle regulation at the MBT and find that the import-limited H3 dynamics contribute to increased robustness and allow for stepwise cell-cycle slowing at the MBT.
(Copyright © 2021 Biophysical Society. Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: P20 GM113132 United States GM NIGMS NIH HHS; P40 OD018537 United States OD NIH HHS
تواريخ الأحداث: Date Created: 20210522 Date Completed: 20211028 Latest Revision: 20221007
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC8516639
DOI: 10.1016/j.bpj.2021.05.005
PMID: 34022240
قاعدة البيانات: MEDLINE
الوصف
تدمد:1542-0086
DOI:10.1016/j.bpj.2021.05.005