دورية أكاديمية
Iso-mukaadial acetate and ursolic acid acetate inhibit the chaperone activity of Plasmodium falciparum heat shock protein 70-1.
العنوان: | Iso-mukaadial acetate and ursolic acid acetate inhibit the chaperone activity of Plasmodium falciparum heat shock protein 70-1. |
---|---|
المؤلفون: | Salomane N; Department of Biochemistry, Faculty of Science, University of Johannesburg, Johannesburg, 2006, South Africa., Pooe OJ; Discipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Westville, Durban, 4000, South Africa., Simelane MBC; Department of Biochemistry, Faculty of Science, University of Johannesburg, Johannesburg, 2006, South Africa. msimelane@uj.ac.za. |
المصدر: | Cell stress & chaperones [Cell Stress Chaperones] 2021 Jul; Vol. 26 (4), pp. 685-693. Date of Electronic Publication: 2021 May 23. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9610925 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1466-1268 (Electronic) Linking ISSN: 13558145 NLM ISO Abbreviation: Cell Stress Chaperones Subsets: MEDLINE |
أسماء مطبوعة: | Publication: 2024- : [New York] : Elsevier Original Publication: New York : Churchill Livingstone, c1996- |
مواضيع طبية MeSH: | HSP70 Heat-Shock Proteins/*drug effects , HSP70 Heat-Shock Proteins/*metabolism , Molecular Chaperones/*metabolism , Plasmodium falciparum/*metabolism , Triterpenes/*pharmacology, Adenosine Triphosphatases/metabolism ; Antimalarials/chemistry ; Antimalarials/metabolism ; Antimalarials/pharmacology ; Escherichia coli/metabolism ; Plasmodium falciparum/drug effects ; Prospective Studies ; Recombinant Proteins/chemistry ; Recombinant Proteins/metabolism ; Ursolic Acid |
مستخلص: | Plasmodium falciparum is the most lethal malaria parasite. The present study investigates the interaction capabilities of select plant derivatives, iso-mukaadial acetate (IMA) and ursolic acid acetate (UAA), against P. falciparum Hsp70-1 (PfHsp70-1) using in vitro approaches. PfHsp70-1 facilitates protein folding in the parasite and is deemed a prospective antimalarial drug target. Recombinant PfHsp70-1 protein was expressed in E. coli BL21 cells and homogeneously purified by affinity chromatography. The interaction between the compounds and PfHsp70-1 was evaluated using malate dehydrogenase (MDH), and luciferase aggregation assay, ATPase activity assay, and Fourier transform infrared (FTIR). PfHsp70-1 prevented the heat-induced aggregation of MDH and luciferase. However, the PfHsp70-1 chaperone role was inhibited by IMA or UAA, leading to both MDH and luciferase's thermal aggregation. The basal ATPase activity of PfHsp70-1 (0.121 nmol/min/mg) was closer to UAA (0.131 nmol/min/mg) (p = 0.0675) at 5 mM compound concentration, suggesting that UAA has no effect on PfHsp70-1 ATPase activity. However, ATPase activity inhibition was similar between IMA (0.068 nmol/min/mg) (p < 0.0001) and polymyxin B (0.083 nmol/min/mg) (p < 0.0001). The lesser the Pi values, the lesser ATP hydrolysis observed due to compound binding to the ATPase domain. FTIR spectra analysis of IMA and UAA resulted in PfHsp70-1 structural alteration for β-sheets shifting the amide I band from 1637 cm -1 to 1639 cm -1 , and for α-helix from 1650 cm -1 to 1652 cm -1 , therefore depicting secondary structural changes with an increase in secondary structure percentage suggesting that these compounds interact with PfHsp70-1. (© 2021. Cell Stress Society International.) |
References: | Protein Pept Lett. 2008;15(10):1117-25. (PMID: 19075824) Protein Sci. 2007 Sep;16(9):1803-18. (PMID: 17766381) Cell Stress Chaperones. 2017 Sep;22(5):707-715. (PMID: 28455613) J Biol Chem. 2012 Feb 17;287(8):5661-72. (PMID: 22157767) Protein Pept Lett. 2017;24(6):503-510. (PMID: 28201964) Front Neurosci. 2018 Nov 12;12:821. (PMID: 30483047) Anal Biochem. 1985 Mar;145(2):230-4. (PMID: 4014655) Drug Des Devel Ther. 2020 Aug 11;14:3235-3249. (PMID: 32884235) Acta Biochim Biophys Sin (Shanghai). 2007 Aug;39(8):549-59. (PMID: 17687489) Antioxid Redox Signal. 2010 Dec 1;13(11):1763-811. (PMID: 20446769) Malar J. 2020 May 18;19(1):183. (PMID: 32423415) J Neurooncol. 2016 May;127(3):463-72. (PMID: 26874961) Mol Aspects Med. 2011 Aug;32(4-6):279-304. (PMID: 22020114) Nat Rev Mol Cell Biol. 2010 Aug;11(8):579-92. (PMID: 20651708) Int J Biochem Cell Biol. 2007;39(10):1781-803. (PMID: 17428722) Antioxidants (Basel). 2020 Sep 03;9(9):. (PMID: 32899274) Biophys J. 2012 Sep 5;103(5):1020-7. (PMID: 23009851) J Ethnopharmacol. 2019 Dec 5;245:112176. (PMID: 31446074) Biophys Chem. 2009 Jun;142(1-3):55-64. (PMID: 19339102) Molecules. 2013 Oct 08;18(10):12313-23. (PMID: 24108397) Eur J Med Chem. 2021 May 5;217:113330. (PMID: 33744688) Proteins. 2018 Nov;86(11):1189-1201. (PMID: 30183110) J Mol Biol. 2019 Jul 12;431(15):2729-2746. (PMID: 31125567) Protein Expr Purif. 2004 Feb;33(2):214-22. (PMID: 14711509) Malar J. 2018 Aug 15;17(1):296. (PMID: 30111328) Protein Expr Purif. 2011 Jun;77(2):159-65. (PMID: 21262365) Front Mol Biosci. 2015 Jun 26;2:34. (PMID: 26167469) Biomolecules. 2019 Jul 23;9(7):. (PMID: 31340488) Biomolecules. 2019 Dec 11;9(12):. (PMID: 31835879) Ageing Res Rev. 2008 Jan;7(1):1-7. (PMID: 18162444) PLoS One. 2015 Aug 12;10(8):e0135326. (PMID: 26267894) |
فهرسة مساهمة: | Keywords: Heat shock protein; Iso-mukaadial acetate; Plasmodium falciparum; Polymyxin B; Ursolic acid acetate |
المشرفين على المادة: | 0 (Antimalarials) 0 (HSP70 Heat-Shock Proteins) 0 (Molecular Chaperones) 0 (Recombinant Proteins) 0 (Triterpenes) 0 (heat-shock protein 70.1) EC 3.6.1.- (Adenosine Triphosphatases) |
تواريخ الأحداث: | Date Created: 20210523 Date Completed: 20220125 Latest Revision: 20240209 |
رمز التحديث: | 20240209 |
مُعرف محوري في PubMed: | PMC8275760 |
DOI: | 10.1007/s12192-021-01212-6 |
PMID: | 34023985 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1466-1268 |
---|---|
DOI: | 10.1007/s12192-021-01212-6 |