دورية أكاديمية
Evaluation of the PBP2 transglycosylase region of Staphylococcus aureus as a target for immunotherapeutic approaches.
| العنوان: | Evaluation of the PBP2 transglycosylase region of Staphylococcus aureus as a target for immunotherapeutic approaches. |
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| المؤلفون: | Corrêa Argondizzo AP; Recombinant Technology Laboratory, Technological Development Department, Instituto de Tecnologia Em Imunobiológicos BioManguinhos FIOCRUZ, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil., Saraiva FB; Recombinant Technology Laboratory, Technological Development Department, Instituto de Tecnologia Em Imunobiológicos BioManguinhos FIOCRUZ, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil., Almeida M; Instituto Oswaldo Cruz - IOC FIOCRUZ, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil., Nunes Peres AM; Assessoria Clínica, Instituto de Tecnologia Em Imunobiológicos BioManguinhos FIOCRUZ, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil., Moreno Senna JP; Recombinant Technology Laboratory, Technological Development Department, Instituto de Tecnologia Em Imunobiológicos BioManguinhos FIOCRUZ, Avenida Brasil, 4365, Manguinhos, Rio de Janeiro, RJ, 21040-900, Brazil. Electronic address: jprocopio@bio.fiocruz.br. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2021 Aug; Vol. 157, pp. 105000. Date of Electronic Publication: 2021 May 25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Methicillin-Resistant Staphylococcus aureus* , Staphylococcus aureus*, Animals ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Bacterial Proteins ; Escherichia coli ; Immunotherapy ; Mice ; Penicillin-Binding Proteins/genetics |
| مستخلص: | Infections caused by Staphylococcus aureus are increasingly prevalent, and treatment has become more difficult due to the emergence of strains that are resistant to multiple drugs, such as methicillin-resistant Staphylococcus aureus (MRSA). Penicillin-binding proteins (PBPs) are essential enzymes in peptidoglycan biosynthesis. Only found in bacteria, they are an excellent target for the development of bacterial control strategies. S. aureus has 4 PBPs, and only PBP2 has transglycosylation activity, making it a good model to evaluate whether the inactivation of the transglycosylase domain (PBP2t) could lead to bacterial death. (His6)-tagged PBP2t was purified from the E. coli cell lysate using Ni-charged resin, and ELISA and immunoblotting assays demonstrated that PBP2t is immunogenic. Flow cytometry analysis was performed to verify the binding of polyclonal antibodies to the bacterial cell surface. In order to verify the ability to provide protection, immunized mice were challenged with a sublethal dose of MRSA, and the bacterial loads in kidneys and spleen were evaluated. A reduction of 2-2.5 logs was seen in organs from immunized mice compared with the negative controls in two independent assays (p < 0.01). Our results demonstrate that the PBP2t is a promising target for the development of novel antimicrobial strategies, but further testing should be performed to validate the protection conferred by immunization with this protein. (Copyright © 2021 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antibiotic resistance; Immunization; PBP2; Staphylococcus aureus; Transglycosylase domain of penicillin binding protein 2 (PBP2) |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Bacterial Proteins) 0 (Penicillin-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20210528 Date Completed: 20210621 Latest Revision: 20210621 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.micpath.2021.105000 |
| PMID: | 34048888 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2021.105000 |