دورية أكاديمية
أعرض في EDS

High-content siRNA 3D co-cultures to identify myoepithelial cell-derived breast cancer suppressor proteins.

التفاصيل البيبلوغرافية
العنوان: High-content siRNA 3D co-cultures to identify myoepithelial cell-derived breast cancer suppressor proteins.
المؤلفون: Duivenvoorden HM; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, 3086, Australia.; School of Biological Sciences, Monash University, VIC, Clayton, 3168, Australia., Brockwell NK; Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australia.; Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, VIC, 3052, Australia., Nowell CJ; Drug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne, VIC, 3052, Australia., Simpson KJ; Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, VIC, 3052, Australia. kaylene.simpson@petermac.org.; Victorian Centre for Functional Genomic, Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australia. kaylene.simpson@petermac.org., Parker BS; Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, 3086, Australia. belinda.parker@petermac.org.; Cancer Immunology Program, Peter MacCallum Cancer Centre, Melbourne, VIC, 3000, Australia. belinda.parker@petermac.org.; Sir Peter MacCallum Department of Oncology, University of Melbourne, Parkville, VIC, 3052, Australia. belinda.parker@petermac.org.
المصدر: Scientific data [Sci Data] 2021 May 28; Vol. 8 (1), pp. 147. Date of Electronic Publication: 2021 May 28.
نوع المنشور: Dataset; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101640192 Publication Model: Electronic Cited Medium: Internet ISSN: 2052-4463 (Electronic) Linking ISSN: 20524463 NLM ISO Abbreviation: Sci Data Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, 2014-
مواضيع طبية MeSH: RNA, Small Interfering*, Breast Neoplasms/*genetics , Breast Neoplasms/*pathology , Tumor Suppressor Proteins/*genetics, Cell Line, Tumor ; Coculture Techniques ; Epithelial Cells/metabolism ; Epithelial Cells/pathology ; Female ; Gene Knockdown Techniques ; Humans ; Neoplasm Invasiveness
مستخلص: Understanding how cancer cells interact with the surrounding microenvironment early in breast cancer development can provide insight into the initiation and progression of invasive breast cancers. The myoepithelial cell layer surrounding breast ducts acts as a physical barrier in early breast cancer, preventing cancer cells from invading the surrounding stroma. Changes to the expression profile and properties of myoepithelial cells have been implicated in progression to invasive carcinoma. Identifying the molecular drivers of myoepithelial cell-mediated tumour suppression may offer new approaches to predict and block the earliest stages of cancer invasion. We employed a high-content approach to knock down 87 different genes using siRNA in an immortalised myoepithelial cell line, prior to co-culture with invasive breast cancer cells in 3D. Combined with high-content imaging and a customised analysis pipeline, this system was used to identify myoepithelial proteins that are necessary to control cancer cell invasion. This dataset has identified prospective myoepithelial suppressors of early breast cancer invasion which may be used by researchers to investigate their clinical validity and utility.
References: Dev Biol. 1999 Jul 15;211(2):238-54. (PMID: 10395785)
Breast Cancer Res Treat. 2011 Aug;129(1):165-73. (PMID: 21390494)
Mol Cancer Res. 2003 Mar;1(5):362-75. (PMID: 12651909)
Cancer Cell. 2004 Jul;6(1):17-32. (PMID: 15261139)
Cell Cycle. 2007 Jan 1;6(1):60-4. (PMID: 17245112)
Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772)
J Mammary Gland Biol Neoplasia. 2005 Jul;10(3):261-72. (PMID: 16807805)
Front Biosci (Landmark Ed). 2010 Jan 01;15:226-36. (PMID: 20036817)
Breast Cancer Res Treat. 2006 May;97(2):135-44. (PMID: 16319971)
J Cell Sci. 2007 Mar 15;120(Pt 6):1050-60. (PMID: 17327279)
Sci Data. 2021 May 28;8(1):147. (PMID: 34050191)
J Pathol. 2017 Dec;243(4):496-509. (PMID: 29086922)
Mol Cell Proteomics. 2012 Aug;11(8):255-71. (PMID: 22448045)
المشرفين على المادة: 0 (RNA, Small Interfering)
0 (Tumor Suppressor Proteins)
تواريخ الأحداث: Date Created: 20210529 Date Completed: 20211015 Latest Revision: 20211015
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8163786
DOI: 10.1038/s41597-021-00924-9
PMID: 34050191
قاعدة البيانات: MEDLINE
الوصف
تدمد:2052-4463
DOI:10.1038/s41597-021-00924-9