دورية أكاديمية
Detection of respiratory viruses in primary cholesteatoma tissues.
| العنوان: | Detection of respiratory viruses in primary cholesteatoma tissues. |
|---|---|
| المؤلفون: | Viana RMM; Department of Ophthalmology, Otorhinolaryngology, and Head and Neck Surgery, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Souza JP; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Jorge DMM; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Martins RB; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Castro IA; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Cardoso RS; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Volpini LPB; Post-Graduate Program in infectious Diseases, Federal University of Espírito Santo, Vitória, Brazil., de Souza Luna LK; Medicine Department, Clinical Virology Laboratory, Infectious Diseases Unit, São Paulo Federal University, São Paulo, Brazil., Spano LC; Post-Graduate Program in infectious Diseases, Federal University of Espírito Santo, Vitória, Brazil.; Departament of Pathology, Center of Health Sciences, Federal University of Espírito Santo, Vitória, Brazil., Bellei NCJ; Medicine Department, Clinical Virology Laboratory, Infectious Diseases Unit, São Paulo Federal University, São Paulo, Brazil., Chahud F; Department of Pathology and Forensic Medicine, Ribeirão Preto School of Medicine, University of São Paulo (USP), São Paulo, Brazil., Arruda E; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., A Hyppolito M; Department of Ophthalmology, Otorhinolaryngology, and Head and Neck Surgery, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil. |
| المصدر: | Journal of medical virology [J Med Virol] 2021 Nov; Vol. 93 (11), pp. 6132-6139. Date of Electronic Publication: 2021 Jun 10. |
| نوع المنشور: | Journal Article; Observational Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 7705876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-9071 (Electronic) Linking ISSN: 01466615 NLM ISO Abbreviation: J Med Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Wiley-Liss Original Publication: New York, Liss. |
| مواضيع طبية MeSH: | Cholesteatoma/*virology , Enterovirus/*isolation & purification , Human bocavirus/*isolation & purification , Metapneumovirus/*isolation & purification , Rhinovirus/*isolation & purification, Adolescent ; Adult ; Aged ; Brazil ; Cholesteatoma/pathology ; Cross-Sectional Studies ; Enterovirus/genetics ; Female ; Human bocavirus/genetics ; Humans ; Male ; Metapneumovirus/genetics ; Middle Aged ; Real-Time Polymerase Chain Reaction ; Rhinovirus/genetics ; Young Adult |
| مستخلص: | Cholesteatomas are frequent middle ear benign tumors of unknown etiology. Infectious agents have been considered as possible contributing factors in the pathogenesis of cholesteatomas. Aiming to investigate the presence of respiratory viruses in primary cholesteatoma tissues, 26 formalin-fixed paraffin-embedded primary cholesteatoma tissues obtained from patients seen at the of the Clinical Hospital of the University of São Paulo School of Medicine, in Ribeirão Preto, Brazil were tested by real-time polymerase chain reaction (PCR). Considering the PCR results, 35% of the tissues were positive for human rhinovirus (HRV), 15.3% for human enterovirus (EV), 3.8% for human metapneumovirus (HMPV), and 3.8% for human bocavirus (HBoV). Serial immunohistochemistry for virus antigens and cell surface markers evidenced that the viruses were associated with fibroblasts, dendritic cells, macrophages, B lymphocytes, CD4 + , and CD8 + T lymphocytes. These findings indicate for the first time the presence of active respiratory virus infection in primary cholesteatoma tissues, suggesting that persisting virus infection in the middle could play a role in the pathogenesis and evolution of cholesteatomas. (© 2021 Wiley Periodicals LLC.) |
| References: | Masanta WO , Hinz R , Zautner AE . Infectious causes of cholesteatoma and treatment of infected ossicles prior to reimplantation by hydrostatic high-pressure inactivation. BioMed Res Int. 2015;(7):1-7. Olszewska E , Wagner M , Bernal-Sprekelsen M , et al. Etiopathogenesis of cholesteatoma. Eur Arch Oto-Rhino-L. 2004;261(1):6-24. Netto CS . Tratado de Otorrinolaringologia e Cirurgia Cervicofacial. 2th ed. São Paulo: Roca; 2011. Müller J . Ueber den feineren Bau und die formen der krankhaften Geschwulste. Berlin: G.Reimer; 1838. Ferekidis E , Nikolopoulos TP , Yiotakis J , et al. Correlation of clinical and surgical findings to histological features (koilocytosis, papillary hyperplasia) suggesting papillomavirus involvement in the pathogenesis of cholesteatoma. Med Sci Monit. 2006;12(9):368-372. Nevoux J , Lenoir M , Roger G , Denoyelle F , Ducou Le Pointe H , Garabédian EN . Childhood cholesteatoma. Eur Ann Otorhinolaryngol Head Neck Dis. 2010;127(4):143-150. Rosito LPS , da Silva MNL , Selaimen FA , et al. Characteristics of 419 patients with acquired middle ear cholesteatoma. Rev Bras Otorrinolaringol. 2017;83(2):126-131. Buzatto GP , Tamashiro E , Proenca-Modena JL , et al. The pathogens profile in children with otitis media with effusion and adenoid hypertrophy. PLoS ONE. 2017;12(2):1-12. Pitkäranta A , Jero J , Arruda E , Virolainen A , Hayden FG . Polymerase chain reaction-based detection of rhinovirus, respiratory syncytial virus, and coronavirus in otitis media with effusion. J Pediatr. 1998;133(3):390-394. Proenca-Modena JL , Pereira Valera FC , Jacob MG , et al. High rates of detection of respiratory viruses in tonsillar tissues from children with chronic adenotonsillar disease. PLoS ONE. 2012;7(8):1-11. Franz P , Teschendorf M , Wohlschläger J , Fischer M . Prevalence of human papillomavirus DNA in cholesteatomas. Orl. 2007;69(4):251-255. Gaynor AM , Nissen MD , Whiley DM , et al. Identification of a novel polyomavirus from patients with acute respiratory tract infections. PLoS Pathogens. 2007;3(5):0595-0604. Wattier RL , Vázquez M , Weibel C , et al. Role of human polyomaviruses in respiratory tract disease in young children. Emerg Infect Dis. 2008;14(11):1766-1768. Pikor LA , Enfield KSS , Cameron H , Lam WL . DNA extraction from paraffin embedded material for genetic and epigenetic analyses. J Vis Exp. 2011;49:15-17. Martins RB , Rocha LP , Prates MM , et al. Respiratory DNA viruses are undetectable in nasopharyngeal secretions from adenotonsillectomized children. PLoS ONE. 2017;12(3):1-7. Gravitt PE , Peyton CL , Alessi TQ , et al. Improved amplification of genital human papillomaviruses. J Clin Microbiol. 2000;38(1):357-361. Lindau C , Tiveljung-Lindell A , Goh S , Ramqvist T , Allander T . A single-tube, real-time PCR assay for detection of the two newly characterized human KI and WU polyomaviruses. J Clin Virol. 2009;44(1):24-26. Glass G , Papin JA , Mandell JW . SIMPLE: a sequential immunoperoxidase labeling and erasing method. J Histochem Cytochem. 2009;57(10):899-905. Wright ED , Pearl AJ , Manoukian JJ . Laterally hypertrophic adenoids as a contributing factor in otitis media. Int J Pediatr Otorhinolaryngol. 1998;45(3):207-214. Nistico L , Kreft R , Gieseke A , et al. Adenoid reservoir for pathogenic biofilm bacteria. J Clin Microbiol. 2011;49(4):1411-1420. Chole RA , Faddis BT . Anatomical evidence of microbial biofilms in tonsillar tissues: a possible mechanism to explain chronicity. Arch Otolaryngol Head Neck Surg. 2003;129(6):634-636. Gates GA , Avery CA , Prihoda TJ , Cooper JC . Effectiveness of Adenoidectomy and Tympanostomy Tubes in the Treatment of Chronic Otitis Media with Effusion. N Engl J Med. 1987;317(23):1444-1451. Paradise JL , Bluestone CD , Rogers KD , et al. Efficacy of Adenoidectomy for Recurrent Otitis Media in Children Previously Treated With Tympanostomy-Tube Placement: results of Parallel Randomized and Nonrandomized Trials. JAMA. 1990;263(15):2066-2073. Vranic S , Cyprian FS , Akhtar S , Al , Moustafa AE . The role of Epstein-Barr virus in cervical cancer: a brief update. Front Oncol. 2018;8(13):1-8. Zur Hausen H . Papillomaviruses in the causation of human cancers - a brief historical account. Virology. 2009;384(2):260-265. Söderberg-Nauclér C , Johnsen JI . Cytomegalovirus infection in brain tumors: a potential new target for therapy? OncoImmunology. 2012;1(5):739-740. |
| فهرسة مساهمة: | Keywords: cell susceptibility; oncogenes; reservoir |
| تواريخ الأحداث: | Date Created: 20210529 Date Completed: 20220207 Latest Revision: 20220207 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/jmv.27107 |
| PMID: | 34050944 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-9071 |
|---|---|
| DOI: | 10.1002/jmv.27107 |