دورية أكاديمية
أعرض في EDS

Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging.

التفاصيل البيبلوغرافية
العنوان: Biocompatible Ir(III) Complexes as Oxygen Sensors for Phosphorescence Lifetime Imaging.
المؤلفون: Kritchenkov IS; Institute of Chemistry, St. Petersburg State University, Universitetskii av., 26, 198504 St. Petersburg, Russia., Solomatina AI; Institute of Chemistry, St. Petersburg State University, Universitetskii av., 26, 198504 St. Petersburg, Russia., Kozina DO; Institute of Chemistry, St. Petersburg State University, Universitetskii av., 26, 198504 St. Petersburg, Russia., Porsev VV; Institute of Chemistry, St. Petersburg State University, Universitetskii av., 26, 198504 St. Petersburg, Russia., Sokolov VV; Institute of Chemistry, St. Petersburg State University, Universitetskii av., 26, 198504 St. Petersburg, Russia., Shirmanova MV; Institute of Experimental Oncology and Biomedical Technologies, Privolzhskiy Research Medical University, Minin and Pozharsky sq. 10/1, 603005 Nizhny Novgorod, Russia., Lukina MM; Institute of Experimental Oncology and Biomedical Technologies, Privolzhskiy Research Medical University, Minin and Pozharsky sq. 10/1, 603005 Nizhny Novgorod, Russia., Komarova AD; Institute of Experimental Oncology and Biomedical Technologies, Privolzhskiy Research Medical University, Minin and Pozharsky sq. 10/1, 603005 Nizhny Novgorod, Russia., Shcheslavskiy VI; Institute of Experimental Oncology and Biomedical Technologies, Privolzhskiy Research Medical University, Minin and Pozharsky sq. 10/1, 603005 Nizhny Novgorod, Russia.; Becker&Hickl GmbH, Nunsdorfer Ring 7-9, 12277 Berlin, Germany., Belyaeva TN; Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky av. 4, 194064 St. Petersburg, Russia., Litvinov IK; Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky av. 4, 194064 St. Petersburg, Russia., Salova AV; Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky av. 4, 194064 St. Petersburg, Russia., Kornilova ES; Institute of Cytology of the Russian Academy of Sciences, Tikhoretsky av. 4, 194064 St. Petersburg, Russia.; Institute of Biomedical Systems and Biotechnology, Peter the Great St. Petersburg Polytechnical University, Khlopina Str. 11, 194021 St. Petersburg, Russia., Kachkin DV; Faculty of Biology, St. Petersburg State University, Universitetskaya emb., 7/9, 199034 St. Petersburg, Russia., Tunik SP; Institute of Chemistry, St. Petersburg State University, Universitetskii av., 26, 198504 St. Petersburg, Russia.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2021 May 13; Vol. 26 (10). Date of Electronic Publication: 2021 May 13.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Luminescence*, Biocompatible Materials/*chemistry , Iridium/*chemistry , Oxygen/*analysis, Animals ; CHO Cells ; Cricetulus ; HeLa Cells ; Humans ; Molecular Conformation ; Proton Magnetic Resonance Spectroscopy ; Subcellular Fractions/metabolism
مستخلص: Synthesis of biocompatible near infrared phosphorescent complexes and their application in bioimaging as triplet oxygen sensors in live systems are still challenging areas of organometallic chemistry. We have designed and synthetized four novel iridium [Ir(N^C) 2 (N^N)] + complexes (N^C-benzothienyl-phenanthridine based cyclometalated ligand; N^N-pyridin-phenanthroimidazol diimine chelate), decorated with oligo(ethylene glycol) groups to impart these emitters' solubility in aqueous media, biocompatibility, and to shield them from interaction with bio-environment. These substances were fully characterized using NMR spectroscopy and ESI mass-spectrometry. The complexes exhibited excitation close to the biological "window of transparency", NIR emission at 730 nm, and quantum yields up to 12% in water. The compounds with higher degree of the chromophore shielding possess low toxicity, bleaching stability, absence of sensitivity to variations of pH, serum, and complex concentrations. The properties of these probes as oxygen sensors for biological systems have been studied by using phosphorescence lifetime imaging experiments in different cell cultures. The results showed essential lifetime response onto variations in oxygen concentration (2.0-2.3 μs under normoxia and 2.8-3.0 μs under hypoxia conditions) in complete agreement with the calibration curves obtained "in cuvette". The data obtained indicate that these emitters can be used as semi-quantitative oxygen sensors in biological systems.
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معلومات مُعتمدة: 18-73-10021 Russian Science Foundation; 20-04-00927 Russian Foundation for Basic Research
فهرسة مساهمة: Keywords: Ir(III) complexes; NIR emitters; oxygen sensing; phosphorescence lifetime imaging
المشرفين على المادة: 0 (Biocompatible Materials)
44448S9773 (Iridium)
S88TT14065 (Oxygen)
تواريخ الأحداث: Date Created: 20210602 Date Completed: 20210624 Latest Revision: 20240402
رمز التحديث: 20240402
مُعرف محوري في PubMed: PMC8153025
DOI: 10.3390/molecules26102898
PMID: 34068190
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules26102898