دورية أكاديمية
Histological Lesions and Replication Sites of PCV3 in Naturally Infected Pigs.
العنوان: | Histological Lesions and Replication Sites of PCV3 in Naturally Infected Pigs. |
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المؤلفون: | De Conti ER; Veterinary School, Federal University of Rio Grande do Sul, Porto Alegre 91540-000, Brazil.; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN 55108, USA., Resende TP; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, Saint Paul, MN 55108, USA., Marshall-Lund L; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN 55108, USA.; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, Saint Paul, MN 55108, USA., Rovira A; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN 55108, USA., Vannucci FA; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN 55108, USA. |
المصدر: | Animals : an open access journal from MDPI [Animals (Basel)] 2021 May 24; Vol. 11 (6). Date of Electronic Publication: 2021 May 24. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Molecular Diversity Preservation International Country of Publication: Switzerland NLM ID: 101635614 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2615 (Print) Linking ISSN: 20762615 NLM ISO Abbreviation: Animals (Basel) Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : Molecular Diversity Preservation International, 2011- |
مستخلص: | Porcine circovirus type 3 (PCV3) has been recently described as a potential cause of abortions and systemic vasculitis in pigs. Although the virus has been detected by real-time PCR in several porcine tissues from countries worldwide, PCV3-associated diseases have not been satisfactorily clarified. The objective of this study was to investigate the association between the presence of PCV3 mRNA detected by in situ hybridization (ISH) within histological lesions and PCV3 DNA detected by real-time PCR in naturally infected pigs. A total of 25 PCV3 PCR-positive cases were analyzed. Formalin-fixed tissues from these cases were evaluated for histologic lesions and for ISH-RNA positive signals for PCV3. The most frequent tissue type with histopathologic lesions was heart, 76.2%, with lymphoplasmacytic myocarditis and epicarditis as the most frequent lesions observed. Lymphoplasmacytic interstitial pneumonia was also a frequent finding, 47.6%. There were also lesions in kidney, liver, spleen and lymph nodes. PCV3-ISH-RNA positive signals were mostly observed in association with lymphoplasmacytic inflammatory infiltrate in various tissues, including arteries. Based on our results, the minimum set of specimens to be submitted for histopathology and mRNA in situ hybridization to confirm or exclude a diagnosis of PCV3 are heart, lung and lymphoid tissues (i.e., spleen and lymph nodes), especially for differential diagnosis related with PCV2-associated diseases. |
التعليقات: | Erratum in: Animals (Basel). 2021 Nov 16;11(11):. (PMID: 34828068) |
References: | Virus Res. 2020 Sep;286:198044. (PMID: 32502553) PLoS One. 2017 Apr 10;12(4):e0173190. (PMID: 28394893) Viruses. 2020 Feb 16;12(2):. (PMID: 32079070) Emerg Microbes Infect. 2019;8(1):684-698. (PMID: 31096848) Virus Res. 2012 Mar;164(1-2):10-9. (PMID: 22056845) Transbound Emerg Dis. 2018 Feb;65(1):5-9. (PMID: 29027372) Virus Genes. 2018 Aug;54(4):608-611. (PMID: 29948781) Virol J. 2016 Nov 11;13(1):184. (PMID: 27835942) Transbound Emerg Dis. 2018 Jun;65(3):602-606. (PMID: 29453822) Vet Pathol. 2015 May;52(3):497-504. (PMID: 24963088) Vet Microbiol. 1995 Apr;44(1):49-64. (PMID: 7667906) J Virol. 2019 Feb 5;93(4):. (PMID: 30487279) J Mol Diagn. 2012 Jan;14(1):22-9. (PMID: 22166544) J Virol. 2016 Dec 16;91(1):. (PMID: 27795441) Transbound Emerg Dis. 2017 Oct;64(5):1350-1353. (PMID: 28649803) Transbound Emerg Dis. 2020 May;67(3):1057-1061. (PMID: 31823481) Porcine Health Manag. 2017 Oct 24;3:25. (PMID: 29085660) Vet Microbiol. 2007 Jan 31;119(2-4):144-51. (PMID: 17070659) Transbound Emerg Dis. 2018 Jun;65(3):607-613. (PMID: 29521007) J Gen Virol. 1998 Sep;79 ( Pt 9):2171-9. (PMID: 9747726) J Vet Diagn Invest. 2019 Jan;31(1):113-117. (PMID: 30541408) Transbound Emerg Dis. 2017 Dec;64(6):1661-1664. (PMID: 28921870) Transbound Emerg Dis. 2020 May;67(3):1062-1067. (PMID: 31880100) J Vet Diagn Invest. 2007 Nov;19(6):591-615. (PMID: 17998548) J Gen Virol. 2021 Jan;102(1):. (PMID: 33206034) J Gen Virol. 2016 Dec;97(12):3267-3279. (PMID: 27902357) Vet Microbiol. 2017 Aug;207:178-180. (PMID: 28757021) |
فهرسة مساهمة: | Keywords: PCV3; histopathology; in situ hybridization |
تواريخ الأحداث: | Date Created: 20210602 Latest Revision: 20220317 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC8224807 |
DOI: | 10.3390/ani11061520 |
PMID: | 34073660 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2076-2615 |
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DOI: | 10.3390/ani11061520 |