دورية أكاديمية
أعرض في EDS

Histological Lesions and Replication Sites of PCV3 in Naturally Infected Pigs.

التفاصيل البيبلوغرافية
العنوان: Histological Lesions and Replication Sites of PCV3 in Naturally Infected Pigs.
المؤلفون: De Conti ER; Veterinary School, Federal University of Rio Grande do Sul, Porto Alegre 91540-000, Brazil.; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN 55108, USA., Resende TP; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, Saint Paul, MN 55108, USA., Marshall-Lund L; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN 55108, USA.; Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, 1971 Commonwealth Avenue, Saint Paul, MN 55108, USA., Rovira A; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN 55108, USA., Vannucci FA; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Minnesota, 1333 Gortner Avenue, Saint Paul, MN 55108, USA.
المصدر: Animals : an open access journal from MDPI [Animals (Basel)] 2021 May 24; Vol. 11 (6). Date of Electronic Publication: 2021 May 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Molecular Diversity Preservation International Country of Publication: Switzerland NLM ID: 101635614 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2615 (Print) Linking ISSN: 20762615 NLM ISO Abbreviation: Animals (Basel) Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : Molecular Diversity Preservation International, 2011-
مستخلص: Porcine circovirus type 3 (PCV3) has been recently described as a potential cause of abortions and systemic vasculitis in pigs. Although the virus has been detected by real-time PCR in several porcine tissues from countries worldwide, PCV3-associated diseases have not been satisfactorily clarified. The objective of this study was to investigate the association between the presence of PCV3 mRNA detected by in situ hybridization (ISH) within histological lesions and PCV3 DNA detected by real-time PCR in naturally infected pigs. A total of 25 PCV3 PCR-positive cases were analyzed. Formalin-fixed tissues from these cases were evaluated for histologic lesions and for ISH-RNA positive signals for PCV3. The most frequent tissue type with histopathologic lesions was heart, 76.2%, with lymphoplasmacytic myocarditis and epicarditis as the most frequent lesions observed. Lymphoplasmacytic interstitial pneumonia was also a frequent finding, 47.6%. There were also lesions in kidney, liver, spleen and lymph nodes. PCV3-ISH-RNA positive signals were mostly observed in association with lymphoplasmacytic inflammatory infiltrate in various tissues, including arteries. Based on our results, the minimum set of specimens to be submitted for histopathology and mRNA in situ hybridization to confirm or exclude a diagnosis of PCV3 are heart, lung and lymphoid tissues (i.e., spleen and lymph nodes), especially for differential diagnosis related with PCV2-associated diseases.
التعليقات: Erratum in: Animals (Basel). 2021 Nov 16;11(11):. (PMID: 34828068)
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فهرسة مساهمة: Keywords: PCV3; histopathology; in situ hybridization
تواريخ الأحداث: Date Created: 20210602 Latest Revision: 20220317
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC8224807
DOI: 10.3390/ani11061520
PMID: 34073660
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-2615
DOI:10.3390/ani11061520