دورية أكاديمية
Antibacterial, antifungal and antioxidant activities of whole plant chemical constituents of Rumex abyssinicus.
| العنوان: | Antibacterial, antifungal and antioxidant activities of whole plant chemical constituents of Rumex abyssinicus. |
|---|---|
| المؤلفون: | Kengne IC; Department of Biochemistry, Research Unit of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Feugap LDT; Department of Chemistry, Research Unit of Applied and Environmental Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Njouendou AJ; Department of Biomedical Science, Faculty of Health Sciences, University of Buea, P.O. Box 12, Buea, Cameroon., Ngnokam CDJ; Department of Chemistry, Research Unit of Applied and Environmental Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Djamalladine MD; Department of Chemistry, Research Unit of Applied and Environmental Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Ngnokam D; Department of Chemistry, Research Unit of Applied and Environmental Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon., Voutquenne-Nazabadioko L; Groupe Isolement et Structure, Institut de Chimie Moléculaire de Reims (ICMR), CNRS UMR 7312, Bat. 18 B.P. 1039, 51687, Reims Cedex 2, France., Tamokou JD; Department of Biochemistry, Research Unit of Microbiology and Antimicrobial Substances, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon. jtamokou@yahoo.fr. |
| المصدر: | BMC complementary medicine and therapies [BMC Complement Med Ther] 2021 Jun 05; Vol. 21 (1), pp. 164. Date of Electronic Publication: 2021 Jun 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101761232 Publication Model: Electronic Cited Medium: Internet ISSN: 2662-7671 (Electronic) Linking ISSN: 26627671 NLM ISO Abbreviation: BMC Complement Med Ther Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : BioMed Central, [2020]- |
| مواضيع طبية MeSH: | Anti-Infective Agents/*pharmacology , Antioxidants/*pharmacology , Plant Extracts/*pharmacology , Rumex/*chemistry, Animals ; Anti-Infective Agents/chemistry ; Antioxidants/chemistry ; Bacteria/drug effects ; Biphenyl Compounds/metabolism ; Cell Survival/drug effects ; Erythrocytes/drug effects ; Fungi/drug effects ; Male ; Phytochemicals/chemistry ; Phytochemicals/pharmacology ; Picrates/metabolism ; Plant Extracts/chemistry ; Rats ; Rats, Wistar |
| مستخلص: | Background: Antibiotic resistance has contributed to the burden of infectious diseases both in the hospital and community setting, and represents a great threat to public health. Previous studies have revealed the role of reactive oxygen species as intermediate mediators of tissue damage, following antibiotherapies, indicating the need of associating antioxidants to these treatments. Therefore, the present work was designed to study the antibacterial, antifungal and antioxidant activities of extracts and compounds from Rumex abyssinicus Jacq. (Polygonaceae), as well as to investigate the antibacterial mechanisms of action of the most effective agents. Methods: The plant extracts were prepared by maceration in organic solvents followed by column chromatography of the EtOAc fraction and purification of different fractions which led to the isolation and characterization of pure compounds. The antimicrobial activities of the extracts/compounds and their combinations with ciprofloxacin and fluconazole were evaluated using the broth microdilution method by determining the minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC). The effects of the extracts on the bacterial cell membrane and microbial respiratory chain dehydrogenase enzyme activity were determined by spectrophotometric methods. Antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and gallic acid equivalent antioxidant capacity (GAEAC) assays. Results: Chrysophanol (1), physcion (2), Ergosta-6,22-diene-3,5,8-triol (3), emodin (4), 6-hydroxyemodin (citreorosein) (5), chrysophanein (6) and physcionin (7) were isolated from EtOAc fraction of R. abyssinicus and displayed different degrees of antimicrobial activities (MIC = 8-256 μg/mL). The MeOH extract and compounds 2 and 4 exhibited synergistic effects with ciprofloxacin and fluconazole. Compounds 1, 2 and the combined mixture of 6 + 7 displayed the highest antioxidant activity (GAEAC = 83.38-106.03 μg/mL). Conclusion: R. abyssinicus is a potential source of antibacterial, antifungal and antioxidant agents. The antibacterial mechanisms of action of the MeOH extract and compound 2 are due to disruption of the cytoplasmic membrane and inhibition of the microbial respiratory chain dehydrogenase enzyme activity. To the best of our knowledge, this is the first report of test samples and ciprofloxacin / fluconazole association against MDR strains. The observed activity of the isolated compounds against bacteria and fungi including MDR strains deserves further exploration. |
| References: | J Ethnopharmacol. 2000 Sep;72(1-2):43-6. (PMID: 10967452) Iran J Med Sci. 2011 Jun;36(2):111-21. (PMID: 23357938) BMC Complement Altern Med. 2018 May 16;18(1):159. (PMID: 29769064) Nat Prod Res. 2009;23(11):1035-49. (PMID: 19521919) Med Princ Pract. 2017;26(2):108-112. (PMID: 27883995) Sci Transl Med. 2013 Jul 3;5(192):192ra85. (PMID: 23825301) J Ethnopharmacol. 2010 Feb 3;127(2):433-9. (PMID: 19854258) Fitoterapia. 2003 Feb;74(1-2):139-43. (PMID: 12628410) PLoS One. 2013;8(2):e55880. (PMID: 23409075) Antimicrob Agents Chemother. 2000 Jun;44(6):1485-93. (PMID: 10817697) Phytother Res. 2009 May;23(5):740-1. (PMID: 19107857) Biometals. 2009 Apr;22(2):235-42. (PMID: 18769871) Chem Cent J. 2018 Sep 20;12(1):97. (PMID: 30238231) J Chromatogr Sci. 2009 Mar;47(3):197-200. (PMID: 19298705) Antimicrob Agents Chemother. 1972 Dec;2(6):470-5. (PMID: 4596743) Methods Enzymol. 1994;234:279-93. (PMID: 7808295) Antimicrob Agents Chemother. 1999 Jul;43(7):1747-53. (PMID: 10390234) BMC Complement Altern Med. 2017 Jan 5;17(1):17. (PMID: 28056944) Eur J Med Chem. 2012 May;51:145-53. (PMID: 22405289) J Ethnopharmacol. 2010 Mar 2;128(1):52-7. (PMID: 20035853) Microbiol Res. 2007;162(3):264-75. (PMID: 16875811) Molecules. 2011 Jan 27;16(2):1201-10. (PMID: 21273951) J Nat Prod. 2002 Aug;65(8):1165-7. (PMID: 12193023) Appl Microbiol. 1975 Jul;30(1):26-8. (PMID: 1147616) J Ethnopharmacol. 2009 Jul 30;124(3):571-5. (PMID: 19464353) Phytother Res. 2005 Oct;19(10):888-94. (PMID: 16261521) Acta Pharmacol Sin. 2016 Dec;37(12):1623-1640. (PMID: 27694907) BMC Complement Altern Med. 2015 Apr 28;15:134. (PMID: 25928352) Nat Prod Res. 2009;23(3):293-300. (PMID: 19235030) Biomolecules. 2019 Feb 18;9(2):. (PMID: 30781696) BMC Complement Altern Med. 2015 Sep 30;15:341. (PMID: 26423525) |
| فهرسة مساهمة: | Keywords: Antimicrobial; Antioxidant; Dehydrogenase activity; Membrane leakage; Multiresistant strains; Rumex abyssinicus |
| المشرفين على المادة: | 0 (Anti-Infective Agents) 0 (Antioxidants) 0 (Biphenyl Compounds) 0 (Phytochemicals) 0 (Picrates) 0 (Plant Extracts) DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl) |
| تواريخ الأحداث: | Date Created: 20210606 Date Completed: 20211221 Latest Revision: 20211221 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8180025 |
| DOI: | 10.1186/s12906-021-03325-y |
| PMID: | 34090405 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2662-7671 |
|---|---|
| DOI: | 10.1186/s12906-021-03325-y |