دورية أكاديمية
Chitosan-based nanofibers for enzyme immobilization.
| العنوان: | Chitosan-based nanofibers for enzyme immobilization. |
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| المؤلفون: | Ribeiro ES; Biotechnology Unit, Technology Development Center, Federal University of Pelotas, Campus Capão do Leão, Capão do Leão, Brazil., de Farias BS; School of Chemistry and Food, Federal University of Rio Grande (FURG), km 8 - Itália Avenue, 96203-900 Rio Grande, Brazil. Electronic address: brunafarias@furg.br., Sant'Anna Cadaval Junior TR; School of Chemistry and Food, Federal University of Rio Grande (FURG), km 8 - Itália Avenue, 96203-900 Rio Grande, Brazil., de Almeida Pinto LA; School of Chemistry and Food, Federal University of Rio Grande (FURG), km 8 - Itália Avenue, 96203-900 Rio Grande, Brazil. Electronic address: dqmpinto@furg.br., Diaz PS; Biotechnology Unit, Technology Development Center, Federal University of Pelotas, Campus Capão do Leão, Capão do Leão, Brazil. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2021 Jul 31; Vol. 183, pp. 1959-1970. Date of Electronic Publication: 2021 Jun 06. |
| نوع المنشور: | Journal Article; Review |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Anti-Infective Agents/*chemistry , Chitosan/*chemistry , Enzymes, Immobilized/*chemistry, Adsorption ; Catalysis ; Enzyme Stability ; Nanofibers ; Porosity |
| مستخلص: | The widespread application of soluble enzymes in industrial processes is considered restrict due to instability of enzymes outside optimum operating conditions. For instance, enzyme immobilization can overcome this issue. In fact, chitosan-based nanofibers have outstanding properties, which can improve the efficiency in enzyme immobilization and the stability of enzymes over a wide range of operating conditions. These properties include biodegradability, antimicrobial activity, non-toxicity, presence of functional groups (amino and hydroxyl), large surface area to volume ratio, enhanced porosity and mechanical properties, easy separations and reusability. Therefore, the present review explores the advantages and drawbacks concerning the different methods of enzyme immobilization, including adsorption, cross-linking and entrapment. All these strategies have questions that still need to be addressed, such as elucidation of adsorption mechanism (physisorption or chemisorption); effect of cross-linking reaction on intramolecular and intermolecular interactions and the effect of internal and external diffusional limitations on entrapment of enzymes. Moreover, the current review discusses the challenges and prospects regarding the application of chitosan-based nanofibers in enzyme immobilization, towards maximizing catalytic activity and lifetime. (Copyright © 2021 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Adsorption; Cross–linking; Electrospinning; Entrapment |
| المشرفين على المادة: | 0 (Anti-Infective Agents) 0 (Enzymes, Immobilized) 9012-76-4 (Chitosan) |
| تواريخ الأحداث: | Date Created: 20210606 Date Completed: 20210728 Latest Revision: 20210728 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ijbiomac.2021.05.214 |
| PMID: | 34090851 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2021.05.214 |