دورية أكاديمية
Isolation and characterisation of colistin-resistant Enterobacterales from chickens in Southeast Nigeria.
| العنوان: | Isolation and characterisation of colistin-resistant Enterobacterales from chickens in Southeast Nigeria. |
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| المؤلفون: | Anyanwu MU; Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka 400001, Enugu State, Nigeria., Marrollo R; Clinical Microbiology Laboratory, IRCCS Arcispedale S. Maria Nuova, AUSL Reggio Emilia, Italy., Paolucci M; Clinical Microbiology Laboratory, IRCCS Arcispedale S. Maria Nuova, AUSL Reggio Emilia, Italy., Brovarone F; Clinical Microbiology Laboratory, IRCCS Arcispedale S. Maria Nuova, AUSL Reggio Emilia, Italy., Nardini P; Clinical Microbiology Laboratory, IRCCS Arcispedale S. Maria Nuova, AUSL Reggio Emilia, Italy., Chah KF; Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka 400001, Enugu State, Nigeria., Shoyinka SVO; Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka 400001, Enugu State, Nigeria., Carretto E; Clinical Microbiology Laboratory, IRCCS Arcispedale S. Maria Nuova, AUSL Reggio Emilia, Italy. Electronic address: edoardo.carretto@ausl.re.it. |
| المصدر: | Journal of global antimicrobial resistance [J Glob Antimicrob Resist] 2021 Sep; Vol. 26, pp. 93-100. Date of Electronic Publication: 2021 Jun 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer Country of Publication: Netherlands NLM ID: 101622459 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2213-7173 (Electronic) Linking ISSN: 22137165 NLM ISO Abbreviation: J Glob Antimicrob Resist Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Published by Elsevier Ltd. on behalf of International Society of Chemotherapy for Infection and Cancer |
| مواضيع طبية MeSH: | Colistin*/pharmacology , Escherichia coli Proteins*/genetics, Animals ; Anti-Bacterial Agents/pharmacology ; Chickens ; Humans ; Multilocus Sequence Typing ; Nigeria ; Random Amplified Polymorphic DNA Technique |
| مستخلص: | Objectives: Resistance to colistin (CST) mediated by mobile genetic elements has had a broad impact worldwide. There is an intensified call for epidemiological surveillance of mcr in different reservoirs to preserve CST for future generations. In Nigeria, the poultry industry is a key livestock sector. This study was undertaken to screen putative colistin-resistant Enterobacterales (CST-r-E) from poultry birds in Southeast Nigeria and to determine the genetic relatedness of mcr-harbouring isolates. Methods: Faecal and cloacal swab samples (n = 785) were collected from chickens in 17 farms located in three contiguous states in Southeast Nigeria between March-November 2018. Following selective culture, CST-r-E were isolated. Confirmation of CST resistance, antimicrobial susceptibility testing, molecular detection of genes mcr-1 to mcr-10, multilocus sequence typing (MLST) and randomly amplified polymorphic DNA (RAPD) analysis were performed on the isolates. A questionnaire was distributed to investigate the knowledge about CST and its use of chicken farm caretakers. Results: Of the 785 samples evaluated, 45 (5.7%) were positive for 48 CST-r-E, among which 23 harboured the mcr-1 gene (22 Escherichia coli and 1 Klebsiella pneumoniae). In two E.coli isolates, a new allelic variant (mcr-1.22) was detected. RAPD analysis allowed the identification of 11 different fingerprints. MLST also revealed 11 STs, with 3 of them being novel. Conclusion: mcr has significantly spread in poultry birds of Southeast Nigeria, which poses a worrisome risk to veterinary and human health. Strategies to prevent indiscriminate use of CST in farms should be quickly adopted before CST resistance becomes a huge global health issue. Competing Interests: Declaration of Competing Interest None declared. (Copyright © 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antimicrobial resistance; Enterobacterales; Mobile colistin resistance; Nigeria; mcr-1; mcr-1.22 |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Escherichia coli Proteins) Z67X93HJG1 (Colistin) |
| تواريخ الأحداث: | Date Created: 20210606 Date Completed: 20211025 Latest Revision: 20211025 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jgar.2021.04.030 |
| PMID: | 34091039 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 2213-7173 |
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| DOI: | 10.1016/j.jgar.2021.04.030 |