دورية أكاديمية
SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia.
| العنوان: | SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia. |
|---|---|
| المؤلفون: | Avetyan D; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia; Institute of Biomedicine and Pharmacy, Russian-Armenian University, Yerevan, 0051, Armenia. Electronic address: d_avetyan@mb.sci.am., Chavushyan A; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia., Ghazaryan H; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia., Melkonyan A; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia., Stepanyan A; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia., Zakharyan R; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia; Institute of Biomedicine and Pharmacy, Russian-Armenian University, Yerevan, 0051, Armenia., Hayrapetyan V; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia., Atshemyan S; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia., Khachatryan G; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia; Institute of Biomedicine and Pharmacy, Russian-Armenian University, Yerevan, 0051, Armenia., Sirunyan T; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia; Institute of Biomedicine and Pharmacy, Russian-Armenian University, Yerevan, 0051, Armenia., Davitavyan S; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia; Institute of Biomedicine and Pharmacy, Russian-Armenian University, Yerevan, 0051, Armenia., Martirosyan G; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia; Davidyants Laboratories, Yerevan, 0054, Armenia., Melik-Andreasyan G; National Center of Disease Control and Prevention, Ministry of Health RA, Yerevan, 0025, Armenia., Sargsyan S; National Center of Disease Control and Prevention, Ministry of Health RA, Yerevan, 0025, Armenia., Ghazazyan A; National Center of Disease Control and Prevention, Ministry of Health RA, Yerevan, 0025, Armenia., Aleksanyan N; National Center of Disease Control and Prevention, Ministry of Health RA, Yerevan, 0025, Armenia., Yin X; Applied Biology Laboratory, Shenyang University of Chemical Technology, Shenyang, 110142, China; Biotech & Biomedicine Science (Shenyang)Co. Ltd, Shenyang, 110000, China., Arakelyan A; Laboratory of Human Genomics and Immunomics, Institute of Molecular Biology, National Academy of Sciences RA, Yerevan, 0014, Armenia. |
| المصدر: | Journal of virological methods [J Virol Methods] 2021 Sep; Vol. 295, pp. 114199. Date of Electronic Publication: 2021 Jun 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 8005839 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0984 (Electronic) Linking ISSN: 01660934 NLM ISO Abbreviation: J Virol Methods Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Amsterdam] : Elsevier/North-Holland Biomedical Press, 1980- |
| مواضيع طبية MeSH: | COVID-19/*diagnosis , COVID-19 Nucleic Acid Testing/*methods , Mass Screening/*methods , SARS-CoV-2/*isolation & purification, Armenia/epidemiology ; COVID-19/epidemiology ; COVID-19/virology ; Humans ; RNA, Viral/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2/genetics ; Sensitivity and Specificity ; Specimen Handling ; Viral Load ; Virus Inactivation |
| مستخلص: | COVID-19 pandemic severely impacted the healthcare and economy on a global scale. It is widely recognized that mass testing is an efficient way to contain the spread of SARS-CoV-2 infection as well as aid in the development of informed policies for disease management. However, the current COVID-19 worldwide infection rates increased the demand for rapid and reliable screening of infection. We compared the performance of qRT-PCR in direct heat-inactivated (H), heat-inactivated and pelleted (HC) samples against RNA in a group of 74 subjects (44 positive and 30 negative). Then we compared the sensitivity of HC in a larger group of 196 COVID-19 positive samples. Our study suggests that HC samples show higher accuracy for SARS-CoV-2 detection PCR assay compared to direct H (89 % vs 83 % of the detection in RNA). The sensitivity of detection using direct samples varied depending on the sample transport and storage media as well as the viral loads (as measured by qRT-PCR Ct levels). Altogether, all the data suggest that purified RNA provides more accurate results, however, direct sample testing with qRT-PCR may help to significantly increase testing capacity. Switching to the direct sample testing is justified if the number of tests is doubled at least. (Copyright © 2021. Published by Elsevier B.V.) |
| References: | Nat Commun. 2020 Sep 23;11(1):4812. (PMID: 32968075) Lancet. 2020 Feb 15;395(10223):470-473. (PMID: 31986257) J Clin Virol. 2020 Aug;129:104519. (PMID: 32629187) PLoS Biol. 2020 Oct 2;18(10):e3000896. (PMID: 33006983) Neoplasma. 1988;35(6):651-5. (PMID: 2851744) J Clin Microbiol. 2020 Jul 23;58(8):. (PMID: 32332061) Sheng Wu Gong Cheng Xue Bao. 2020 Apr 25;36(4):732-739. (PMID: 32347067) Diagn Microbiol Infect Dis. 2020 Sep;98(1):115109. (PMID: 32593875) Gene Ther. 2000 Jun;7(11):914-23. (PMID: 10849550) Emerg Microbes Infect. 2020 Dec;9(1):1393-1396. (PMID: 32552549) PLoS Biol. 2020 Jun 8;18(6):e3000715. (PMID: 32511245) Int J Infect Dis. 2020 Aug;97:66-68. (PMID: 32492531) Nature. 2020 Mar;579(7798):270-273. (PMID: 32015507) Ann Clin Lab Sci. 2020 Sep;50(5):665-669. (PMID: 33067213) J Virol Methods. 2021 Mar;289:114062. (PMID: 33428990) J Clin Virol. 2020 Jul;128:104423. (PMID: 32416598) Nature. 2020 Mar;579(7798):265-269. (PMID: 32015508) |
| فهرسة مساهمة: | Keywords: COVID-19; Direct sample qRT-PCR; Heat-Inactivation; Pelleting; SARS-CoV-2; Virus detection |
| المشرفين على المادة: | 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20210606 Date Completed: 20210723 Latest Revision: 20221217 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8175123 |
| DOI: | 10.1016/j.jviromet.2021.114199 |
| PMID: | 34091213 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0984 |
|---|---|
| DOI: | 10.1016/j.jviromet.2021.114199 |