دورية أكاديمية
Determination of Abundant Metabolite Matrix Adducts Illuminates the Dark Metabolome of MALDI-Mass Spectrometry Imaging Datasets.
العنوان: | Determination of Abundant Metabolite Matrix Adducts Illuminates the Dark Metabolome of MALDI-Mass Spectrometry Imaging Datasets. |
---|---|
المؤلفون: | Janda M; Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany., Seah BKB; Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany., Jakob D; Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany., Beckmann J; Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany., Geier B; Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany., Liebeke M; Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany. |
المصدر: | Analytical chemistry [Anal Chem] 2021 Jun 22; Vol. 93 (24), pp. 8399-8407. Date of Electronic Publication: 2021 Jun 07. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 0370536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6882 (Electronic) Linking ISSN: 00032700 NLM ISO Abbreviation: Anal Chem Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Washington, American Chemical Society. |
مواضيع طبية MeSH: | Metabolome* , Metabolomics*, Diagnostic Imaging ; Lipids ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
مستخلص: | Spatial metabolomics using mass spectrometry imaging (MSI) is a powerful tool to map hundreds to thousands of metabolites in biological systems. One major challenge in MSI is the annotation of m / z values, which is substantially complicated by background ions introduced throughout the chemicals and equipment used during experimental procedures. Among many factors, the formation of adducts with sodium or potassium ions, or in case of matrix-assisted laser desorption ionization (MALDI)-MSI, the presence of abundant matrix clusters strongly increases total m / z peak counts. Currently, there is a limitation to identify the chemistry of the many unknown peaks to interpret their biological function. We took advantage of the co-localization of adducts with their parent ions and the accuracy of high mass resolution to estimate adduct abundance in 20 datasets from different vendors of mass spectrometers. Metabolites ranging from lipids to amines and amino acids form matrix adducts with the commonly used 2,5-dihydroxybenzoic acid (DHB) matrix like [M + (DHB-H |
References: | Nat Methods. 2017 Jan;14(1):90-96. (PMID: 27842060) Int J Mass Spectrom. 2007 Feb 1;260(2-3):212-221. (PMID: 17541451) Anal Bioanal Chem. 2020 Oct;412(25):6875-6886. (PMID: 32712813) Analyst. 2014 Jul 21;139(14):3528-32. (PMID: 24802717) Electrophoresis. 1999 Dec;20(18):3551-67. (PMID: 10612281) Ther Drug Monit. 2005 Dec;27(6):747-51. (PMID: 16404815) J Proteome Res. 2008 Aug;7(8):3619-27. (PMID: 18570456) BMC Bioinformatics. 2006 Apr 28;7:234. (PMID: 16646969) Science. 2015 Apr 10;348(6231):211-5. (PMID: 25745064) Nat Microbiol. 2020 Mar;5(3):498-510. (PMID: 32015496) J Am Soc Mass Spectrom. 2002 Feb;13(2):129-34. (PMID: 11838016) Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):11685-11690. (PMID: 29078340) Metabolomics. 2007 Sep;3(3):211-221. (PMID: 24039616) Malawi Med J. 2012 Sep;24(3):69-71. (PMID: 23638278) Curr Opin Biotechnol. 2015 Aug;34:1-8. (PMID: 25461505) Anal Biochem. 2001 Feb 15;289(2):202-16. (PMID: 11161314) Anal Chim Acta. 2018 Dec 11;1037:13-27. (PMID: 30292286) J Am Soc Mass Spectrom. 2019 Aug;30(8):1426-1434. (PMID: 30993641) Nat Methods. 2017 Jan;14(1):57-60. (PMID: 27842059) Anal Chem. 2016 Nov 15;88(22):10893-10899. (PMID: 27641083) Anal Chem. 2005 Oct 1;77(19):6118-24. (PMID: 16194068) Histochem Cell Biol. 2008 Sep;130(3):421-34. (PMID: 18618129) Bioinformatics. 2008 Jan 1;24(1):143-5. (PMID: 18003642) Bioinformatics. 2015 Jul 15;31(14):2418-20. (PMID: 25777525) Anal Chem. 2017 Aug 15;89(16):8565-8573. (PMID: 28703574) Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):12549-50. (PMID: 26430243) Anal Bioanal Chem. 2017 Feb;409(5):1221-1230. (PMID: 27873003) Nat Chem Biol. 2009 Dec;5(12):885-7. (PMID: 19915536) Rapid Commun Mass Spectrom. 1989 Dec;3(12):436-9. (PMID: 2520224) Histochem Cell Biol. 2013 Jun;139(6):759-83. (PMID: 23652571) Anal Chem. 1996 Jan 1;68(1):31-7. (PMID: 8779435) Metabolomics. 2006;2(3):155-164. (PMID: 24489532) J Am Soc Mass Spectrom. 2013 May;24(5):718-21. (PMID: 23536269) |
المشرفين على المادة: | 0 (Lipids) |
تواريخ الأحداث: | Date Created: 20210607 Date Completed: 20210630 Latest Revision: 20210630 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC8223199 |
DOI: | 10.1021/acs.analchem.0c04720 |
PMID: | 34097397 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1520-6882 |
---|---|
DOI: | 10.1021/acs.analchem.0c04720 |