دورية أكاديمية
Insulinoma-derived pseudo-islets for diabetes research.
| العنوان: | Insulinoma-derived pseudo-islets for diabetes research. |
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| المؤلفون: | Hart NJ; Department of Surgery, University of Arizona, Tucson, Arizona., Weber C; Department of Physiology, University of Arizona, Tucson, Arizona., Price N; Department of Surgery, University of Arizona, Tucson, Arizona., Banuelos A; Department of Surgery, University of Arizona, Tucson, Arizona., Schultz M; Department of Surgery, University of Arizona, Tucson, Arizona., Huey B; Department of Surgery, University of Arizona, Tucson, Arizona., Harnois E; Department of Physiology, University of Arizona, Tucson, Arizona., Gibson C; Department of Surgery, University of Arizona, Tucson, Arizona., Steyn LV; Department of Surgery, University of Arizona, Tucson, Arizona., Papas KK; Department of Surgery, University of Arizona, Tucson, Arizona.; Department of Biomedical Engineering, University of Arizona, Tucson, Arizona.; The BIO5 Institute, University of Arizona, Tucson, Arizona., Lynch RM; Department of Physiology, University of Arizona, Tucson, Arizona.; Department of Biomedical Engineering, University of Arizona, Tucson, Arizona.; The BIO5 Institute, University of Arizona, Tucson, Arizona. |
| المصدر: | American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2021 Aug 01; Vol. 321 (2), pp. C247-C256. Date of Electronic Publication: 2021 Jun 09. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901225 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1563 (Electronic) Linking ISSN: 03636143 NLM ISO Abbreviation: Am J Physiol Cell Physiol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, Md. : American Physiological Society, |
| مواضيع طبية MeSH: | Diabetes Mellitus/*metabolism , Insulinoma/*metabolism , Islets of Langerhans/*metabolism , Pancreas/*metabolism, Animals ; Cell Line ; Glucose/metabolism ; Insulin Secretion/physiology ; Insulin-Secreting Cells/metabolism ; Oxygen Consumption/physiology |
| مستخلص: | The islets of Langerhans of the pancreas are the primary endocrine organ responsible for regulating whole body glucose homeostasis. The use of isolated primary islets for research development and training requires organ resection, careful digestion, and isolation of the islets from nonendocrine tissue. This process is time consuming, expensive, and requires substantial expertise. For these reasons, we sought to develop a more rapidly obtainable and consistent model system with characteristic islet morphology and function that could be employed to train personnel and better inform experiments prior to using isolated rodent and human islets. Immortalized β cell lines reflect several aspects of primary β cells, but cell propagation in monolayer cell culture limits their usefulness in several areas of research, which depend on islet morphology and/or functional assessment. In this manuscript, we describe the propagation and characterization of insulinoma pseudo-islets (IPIs) from a rat insulinoma cell line INS832/3. IPIs were generated with an average diameter of 200 μm, consistent with general islet morphology. The rates of oxygen consumption and mitochondrial oxidation-reduction changes in response to glucose and metabolic modulators were similar to isolated rat islets. In addition, the dynamic insulin secretory patterns of IPIs were similar to primary rat islets. Thus, INS832/3-derived IPIs provide a valuable and convenient model for accelerating islet and diabetes research. |
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| معلومات مُعتمدة: | DP3 DK106933 United States DK NIDDK NIH HHS; 1DP3DK106933-01 HHS | National Institutes of Health (NIH) |
| فهرسة مساهمة: | Keywords: diabetes; insulin; organoids; pseudo-islet; β cell |
| المشرفين على المادة: | IY9XDZ35W2 (Glucose) |
| تواريخ الأحداث: | Date Created: 20210609 Date Completed: 20210922 Latest Revision: 20220802 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8424674 |
| DOI: | 10.1152/ajpcell.00466.2020 |
| PMID: | 34106785 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1563 |
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| DOI: | 10.1152/ajpcell.00466.2020 |