Testing the genomic stability of the Brazilian yellow fever vaccine strain using next-generation sequencing data.

التفاصيل البيبلوغرافية
العنوان:	Testing the genomic stability of the Brazilian yellow fever vaccine strain using next-generation sequencing data.
المؤلفون:	Serrão de Andrade AA; National Laboratory for Scientific Computing, Bioinformatics Laboratory (LABINFO), Avenida Getúlio Vargas, 333, Quitandinha 25651-075, Petrópolis, Rio de Janeiro, Brazil., Soares AER; National Laboratory for Scientific Computing, Bioinformatics Laboratory (LABINFO), Avenida Getúlio Vargas, 333, Quitandinha 25651-075, Petrópolis, Rio de Janeiro, Brazil., Paula de Almeida LG; National Laboratory for Scientific Computing, Bioinformatics Laboratory (LABINFO), Avenida Getúlio Vargas, 333, Quitandinha 25651-075, Petrópolis, Rio de Janeiro, Brazil., Ciapina LP; National Laboratory for Scientific Computing, Bioinformatics Laboratory (LABINFO), Avenida Getúlio Vargas, 333, Quitandinha 25651-075, Petrópolis, Rio de Janeiro, Brazil., Pestana CP; Fiocruz, Bio-Manguinhos, Recombinant Technology Laboratory (LATER), Brazilian Ministry of Health, Rio de Janeiro, Brazil., Aquino CL; Fiocruz, Bio-Manguinhos, Recombinant Technology Laboratory (LATER), Brazilian Ministry of Health, Rio de Janeiro, Brazil., Medeiros MA; Fiocruz, Bio-Manguinhos, Recombinant Technology Laboratory (LATER), Brazilian Ministry of Health, Rio de Janeiro, Brazil., Ribeiro de Vasconcelos AT; National Laboratory for Scientific Computing, Bioinformatics Laboratory (LABINFO), Avenida Getúlio Vargas, 333, Quitandinha 25651-075, Petrópolis, Rio de Janeiro, Brazil.
المصدر:	Interface focus [Interface Focus] 2021 Jun 11; Vol. 11 (4), pp. 20200063. Date of Electronic Publication: 2021 Jun 11 (Print Publication: 2021).
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Royal Society Country of Publication: England NLM ID: 101531990 Publication Model: eCollection Cited Medium: Print ISSN: 2042-8898 (Print) Linking ISSN: 20428898 NLM ISO Abbreviation: Interface Focus Subsets: PubMed not MEDLINE
أسماء مطبوعة:	Original Publication: London : Royal Society, 2011-
مستخلص:	The live attenuated yellow fever (YF) vaccine was developed in the 1930s. Currently, the 17D and 17DD attenuated substrains are used for vaccine production. The 17D strain is used for vaccine production by several countries, while the 17DD strain is used exclusively in Brazil. The cell passages carried out through the seed-lot system of vaccine production influence the presence of quasispecies causing changes in the stability and immunogenicity of attenuated genotypes by increasing attenuation or virulence. Using next-generation sequencing, we carried out genomic characterization and genetic diversity analysis between vaccine lots of the Brazilian YF vaccine, produced by BioManguinhos-Fiocruz, and used during 11 years of vaccination in Brazil. We present 20 assembled and annotated genomes from the Brazilian 17DD vaccine strain, eight single nucleotide polymorphisms and the quasispecies spectrum reconstruction for the 17DD vaccine, through a pipeline here introduced. The V2IDA pipeline provided a relationship between low genetic diversity, maintained through the seed lot system, and the confirmation of genetic stability of lots of the Brazilian vaccine against YF. Our study sets precedents for use of V2IDA in genetic diversity analysis and in silico stability investigation of attenuated viral vaccines, facilitating genetic surveillance during the vaccine production process. (© 2021 The Authors.)
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فهرسة مساهمة:	Keywords: 17DD; attenuated viral vaccines; genetic diversity; genetic stability; yellow fever vaccine
سلسلة جزيئية:	figshare 10.6084/m9.figshare.c.5453960
تواريخ الأحداث:	Date Created: 20210614 Latest Revision: 20220204
رمز التحديث:	20221213
مُعرف محوري في PubMed:	PMC8193464
DOI:	10.1098/rsfs.2020.0063
PMID:	34123353
قاعدة البيانات:	MEDLINE

الوصف
تدمد:	2042-8898
DOI:	10.1098/rsfs.2020.0063