دورية أكاديمية
أعرض في EDS

The contribution of the immune response to enhanced colibacillosis upon preceding viral respiratory infection in broiler chicken in a dual infection model.

التفاصيل البيبلوغرافية
العنوان: The contribution of the immune response to enhanced colibacillosis upon preceding viral respiratory infection in broiler chicken in a dual infection model.
المؤلفون: Weerts EAWS; Division of Pathology, Department Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, the Netherlands. Electronic address: e.a.w.s.weerts@uu.nl., Matthijs MGR; Division of Farm Animal Health, Department Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CL Utrecht, the Netherlands., Bonhof J; Division of Infectious Diseases and Immunology, Department Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, the Netherlands., van Haarlem DA; Division of Infectious Diseases and Immunology, Department Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, the Netherlands., Dwars RM; Division of Farm Animal Health, Department Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 7, 3584 CL Utrecht, the Netherlands., Gröne A; Division of Pathology, Department Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, the Netherlands., Verheije MH; Division of Pathology, Department Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, the Netherlands., Jansen CA; Division of Infectious Diseases and Immunology, Department Biomolecular Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, the Netherlands.
المصدر: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2021 Aug; Vol. 238, pp. 110276. Date of Electronic Publication: 2021 May 27.
نوع المنشور: Journal Article; Randomized Controlled Trial, Veterinary
اللغة: English
بيانات الدورية: Publisher: Elsevier Scientific Country of Publication: Netherlands NLM ID: 8002006 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2534 (Electronic) Linking ISSN: 01652427 NLM ISO Abbreviation: Vet Immunol Immunopathol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] Elsevier Scientific.
مواضيع طبية MeSH: Chickens* , Infectious bursal disease virus*, Birnaviridae Infections/*veterinary , Escherichia coli Infections/*veterinary , Poultry Diseases/*microbiology, Air Sacs/microbiology ; Air Sacs/pathology ; Animals ; Birnaviridae Infections/complications ; Birnaviridae Infections/virology ; Coinfection ; Cytokines ; Escherichia coli ; Escherichia coli Infections/immunology ; Escherichia coli Infections/microbiology ; Female ; Poultry Diseases/immunology ; Poultry Diseases/virology ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Respiratory Tract Infections/microbiology ; Respiratory Tract Infections/veterinary ; Respiratory Tract Infections/virology ; Specific Pathogen-Free Organisms
مستخلص: Colibacillosis in chickens caused by avian pathogenic Escherichia coli (APEC) is known to be aggravated by preceding infections with infectious bronchitis virus (IBV), Newcastle disease virus (NDV) and avian metapneumovirus (aMPV). The mechanism behind these virus-induced predispositions for secondary bacterial infections is poorly understood. Here we set out to investigate the immunopathogenesis of enhanced respiratory colibacillosis after preceding infections with these three viruses. Broilers were inoculated intratracheally with APEC six days after oculonasal and intratracheal inoculation with IBV, NDV, aMPV or buffered saline. After euthanasia at 1 and 8 days post infection (dpi) with APEC, birds were macroscopically examined and tissue samples were taken from the trachea, lungs and air sacs. In none of the groups differences in body weight were observed during the course of infection. Macroscopic lesion scoring revealed most severe tissue changes after NDV-APEC and IBV-APEC infection. Histologically, persistent tracheitis was detected in all virus-APEC groups, but not after APEC-only infection. In the lungs, mostly APEC-associated transient pneumonia was observed. Severe and persistent airsacculitis was present after NDV-APEC and IBV-APEC infection. Bacterial antigen was detected by immunohistochemistry only at 1 dpi APEC, predominantly in NDV-APEC- and IBV-APEC-infected lungs. Higher numbers of CD4+ and CD8+ lymphocytes persisted over time in NDV-APEC- and IBV-APEC-infected tracheas, as did CD4+ lymphocytes in NBV-APEC- and IBV-APEC-infected air sacs. KUL01+ cells, which include monocytes and macrophages, and TCRγδ+ lymphocytes were observed mostly in lung tissue in all infected groups with transient higher numbers of KUL01+ cells over time and higher numbers of TCRγδ+ lymphocytes mainly at 8 dpi. qPCR analysis revealed mostly trends of transient higher levels of IL-6 and IFNγ mRNA in lung tissue after IBV-APEC and also NDV-APEC infection and persistent higher levels of IL-6 mRNA after aMPV-APEC infection. In spleens, transient higher levels of IL-17 mRNA and more persistent higher levels of IL-6 mRNA were observed after all co-infections. No changes in IL-10 mRNA expression were seen. These results demonstrate a major impact of dual infections with respiratory viruses and APEC, compared to a single infection with APEC, on the chicken respiratory tract and suggest that immunopathogenesis contributes to lesion persistence.
(Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Avian metapneumovirus; Avian pathogenic Escherichia coli; Broilers; Dual infection; Infectious bronchitis virus; Newcastle disease virus
المشرفين على المادة: 0 (Cytokines)
0 (RNA, Messenger)
تواريخ الأحداث: Date Created: 20210614 Date Completed: 20211115 Latest Revision: 20211115
رمز التحديث: 20221213
DOI: 10.1016/j.vetimm.2021.110276
PMID: 34126552
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2534
DOI:10.1016/j.vetimm.2021.110276