دورية أكاديمية
أعرض في EDS

Centrosomal P4.1-associated protein (CPAP) positively regulates endocytic vesicular transport and lysosome targeting of EGFR.

التفاصيل البيبلوغرافية
العنوان: Centrosomal P4.1-associated protein (CPAP) positively regulates endocytic vesicular transport and lysosome targeting of EGFR.
المؤلفون: Gudi R; Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, 29425, USA. gudi@musc.edu., Palanisamy V; Department of Biochemistry, Medical University of South Carolina, Charleston, SC, 29425, USA., Vasu C; Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, 29425, USA. vasu@musc.edu.
المصدر: Scientific reports [Sci Rep] 2021 Jun 16; Vol. 11 (1), pp. 12689. Date of Electronic Publication: 2021 Jun 16.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Lysosomes/*metabolism , Microtubule-Associated Proteins/*metabolism , Transport Vesicles/*metabolism, Cell Line, Tumor ; Endocytosis ; Endosomes/metabolism ; ErbB Receptors/metabolism ; HEK293 Cells ; HeLa Cells ; Humans ; Ligands ; Microtubule-Associated Proteins/genetics ; Multivesicular Bodies/metabolism ; Protein Transport
مستخلص: Centrosomal P4.1-associated protein (CPAP) plays a critical role in restricting the centriole length in human cells. Here, we report a novel, positive regulatory influence for CPAP on endocytic vesicular transport (EVT) and lysosome targeting of internalized-cell surface receptor EGFR. We observed that higher CPAP levels cause an increase in the abundance of multi-vesicular body (MVB) and EGFR is detectable in CPAP-overexpression induced puncta. The surface and cellular levels of EGFR are higher under CPAP deficiency and lower under CPAP overexpression. While ligand-engagement induced internalization or routing of EGFR into early endosomes is not influenced by cellular levels of CPAP, we found that targeting of ligand-activated, internalized EGFR to lysosome is impacted by CPAP levels. Transport of ligand-bound EGFR from early endosome to late endosome/MVB and lysosome is diminished in CPAP-depleted cells. Moreover, CPAP depleted cells appear to show a diminished ability to form MVB structures upon EGFR activation. These observations suggest a positive regulatory effect of CPAP on EVT of ligand-bound EGFR-like cell surface receptors to MVB and lysosome. Overall, identification of a non-centriolar function of CPAP in endocytic trafficking provides new insights in understanding the non-canonical cellular functions of CPAP.
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معلومات مُعتمدة: P30 CA138313 United States CA NCI NIH HHS; R21 DE026965 United States DE NIDCR NIH HHS; S10 OD018113 United States OD NIH HHS
المشرفين على المادة: 0 (CENPJ protein, human)
0 (Ligands)
0 (Microtubule-Associated Proteins)
EC 2.7.10.1 (EGFR protein, human)
EC 2.7.10.1 (ErbB Receptors)
تواريخ الأحداث: Date Created: 20210617 Date Completed: 20211029 Latest Revision: 20240505
رمز التحديث: 20240505
مُعرف محوري في PubMed: PMC8209166
DOI: 10.1038/s41598-021-91818-8
PMID: 34135376
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-021-91818-8