دورية أكاديمية
Antibody Surface Coverage Drives Matrix Interference in Microfluidic Capillary Immunoassays.
| العنوان: | Antibody Surface Coverage Drives Matrix Interference in Microfluidic Capillary Immunoassays. |
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| المؤلفون: | Barbosa AI; Department of Chemical Engineering, Loughborough University, Loughborough LE11 3TU, United Kingdom.; Capillary Film Technology Ltd, Daux Road, Billingshurst RH14 9SJ, West Sussex, United Kingdom., Edwards AD; Capillary Film Technology Ltd, Daux Road, Billingshurst RH14 9SJ, West Sussex, United Kingdom.; Reading School of Pharmacy, University of Reading, Whiteknights, Reading RG6 6AD, United Kingdom., Reis NM; Capillary Film Technology Ltd, Daux Road, Billingshurst RH14 9SJ, West Sussex, United Kingdom.; Department of Chemical Engineering and Centre for Biosensors, Bioelectronics and Biodevices (C3Bio), University of Bath, Claverton Down, Bath BA2 7AY, United Kingdom. |
| المصدر: | ACS sensors [ACS Sens] 2021 Jul 23; Vol. 6 (7), pp. 2682-2690. Date of Electronic Publication: 2021 Jun 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101669031 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2379-3694 (Electronic) Linking ISSN: 23793694 NLM ISO Abbreviation: ACS Sens Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : American Chemical Society, [2016]- |
| مواضيع طبية MeSH: | Biosensing Techniques* , Microfluidics*, Antibodies ; Immunoassay ; Point-of-Care Testing |
| مستخلص: | The performance of biosensors is often optimized in buffers, which brings inconsistencies during applications with biological samples. Current strategies for minimizing sample (matrix) interference are complex to automate and miniaturize, involving, e.g., sample dilution or recovery of serum/plasma. This study shows the first systematic analysis using hundreds of actual microfluidic immunoassay fluoropolymer strips to understand matrix interference in microflow systems. As many interfering factors are assay-specific, we have explored matrix interference for a range of enzymatic immunoassays, including a direct mIgG/anti-mIgG, a sandwich cancer biomarker PSA, and a sandwich inflammatory cytokine IL-1β. Serum matrix interference was significantly affected by capillary antibody surface coverage, suggesting for the first time that the main cause of the serum matrix effect is low-affinity serum components (e.g., autoantibodies) competing with high-affinity antigens for the immobilized antibody. Additional experiments carried out with different capillary diameters confirmed the importance of antibody surface coverage in managing matrix interference. Building on these findings, we propose a novel analytical approach where antibody surface coverage and sample incubation times are key for eliminating and/or minimizing serum matrix interference, consisting in bioassay optimization carried out in serum instead of buffer, without compromising the performance of the bioassay or adding extra cost or steps. This will help establishing a new route toward faster development of modern point-of-care tests and effective biosensor development. |
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| فهرسة مساهمة: | Keywords: biosensors; matrix effect; microcapillary film; microfluidics; protein biomarkers |
| المشرفين على المادة: | 0 (Antibodies) |
| تواريخ الأحداث: | Date Created: 20210617 Date Completed: 20210802 Latest Revision: 20240405 |
| رمز التحديث: | 20240405 |
| مُعرف محوري في PubMed: | PMC8741144 |
| DOI: | 10.1021/acssensors.1c00704 |
| PMID: | 34138534 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 2379-3694 |
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| DOI: | 10.1021/acssensors.1c00704 |