| المؤلفون: |
Fava NMN; Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, Avenida Trabalhador São-Carlense 400, São Carlos, São Paulo 13566-590, Brazil E-mail: lysaboga@sc.usp.br., Silva KJS; Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, Avenida Trabalhador São-Carlense 400, São Carlos, São Paulo 13566-590, Brazil E-mail: lysaboga@sc.usp.br., Snelling WJ; Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, Ulster University, Coleraine BT52 1SA, UK., Ternan NG; Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, Ulster University, Coleraine BT52 1SA, UK., Dooley JSG; Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, Ulster University, Coleraine BT52 1SA, UK., Sabogal-Paz LP; Department of Hydraulics and Sanitation, São Carlos School of Engineering, University of São Paulo, Avenida Trabalhador São-Carlense 400, São Carlos, São Paulo 13566-590, Brazil E-mail: lysaboga@sc.usp.br. |
| مستخلص: |
Giardia duodenalis and Cryptosporidium spp. are two of the most prominent aetiological agents of waterborne diseases. Therefore, efficient and affordable methodologies for identifying and quantifying these parasites in water are increasingly necessary. USEPA Method 1623.1 is a widely used and validated protocol for detecting these parasites in water samples. It consists of a concentration step, followed by parasite purification and visualization by immunofluorescence microscopy. Although efficient, this method has a high cost particularly due to the immunomagnetic separation (IMS) step, which is most needed with complex and highly contaminated samples. Based on this, the present study aimed to determine whether it is possible to maintain the efficiency of Method 1623.1 while reducing the amount of beads per reaction, using as a matrix the challenge water recommended by the World Health Organization. As for Giardia cysts, a satisfactory recovery efficiency (RE) was obtained using 50% less IMS beads. This was evaluated both with a commercial cyst suspension (56.1% recovery) and an analytical quality assessment (47.5% recovery). Although RE rates obtained for Cryptosporidium parvum did not meet Method 1623.1 criteria in any of the experimental conditions tested, results presented in this paper indicated the relevance of the described adaptations, even in challenge water. |
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