دورية أكاديمية
Potential application of two cobalt (III) Schiff base complexes in cancer chemotherapy: Leads from a study using breast and lung cancer cells.
| العنوان: | Potential application of two cobalt (III) Schiff base complexes in cancer chemotherapy: Leads from a study using breast and lung cancer cells. |
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| المؤلفون: | Gowdhami B; Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India; National Centre for Alternatives to Animal Experiments, Bharathidasan University, Tiruchirappalli, Tamilnadu, India., Ambika S; Department of Chemistry, Bishop Heber College, Tiruchirappalli, Tamilnadu, India., Karthiyayini B; National Centre for Alternatives to Animal Experiments, Bharathidasan University, Tiruchirappalli, Tamilnadu, India; Department of Microbiology, Bharathidasan University, Tiruchirappalli, Tamilnadu, India., Ramya V; Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India., Kadalmani B; Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu, India. Electronic address: kadalmani@bdu.ac.in., Vimala RTV; Department of Biotechnology, Bharathidasan University, Tiruchirappalli, Tamilnadu, India., Akbarsha MA; Mahatma Gandhi-Dorenkamp Centre, Bharathidasan University, Tiruchirappalli, Tamilnadu, India; Department of Biotechnology & Research Coordinator, National College (Autonomous), Tiruchirappalli, Tamilnadu, India. Electronic address: akbarbdu@gmail.com. |
| المصدر: | Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2021 Sep; Vol. 75, pp. 105201. Date of Electronic Publication: 2021 Jun 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon Press Country of Publication: England NLM ID: 8712158 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3177 (Electronic) Linking ISSN: 08872333 NLM ISO Abbreviation: Toxicol In Vitro Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford ; New York : Pergamon Press, c1987- |
| مواضيع طبية MeSH: | Antineoplastic Agents/*pharmacology , Breast Neoplasms/*drug therapy , Cobalt/*pharmacology , Coordination Complexes/*pharmacology , Lung Neoplasms/*drug therapy , Schiff Bases/*pharmacology, A549 Cells ; Apoptosis/drug effects ; Breast Neoplasms/genetics ; Breast Neoplasms/metabolism ; Cell Proliferation/drug effects ; DNA Damage ; Female ; Gene Expression Regulation, Neoplastic/drug effects ; Humans ; Lung Neoplasms/genetics ; Lung Neoplasms/metabolism ; MCF-7 Cells ; Membrane Potential, Mitochondrial/drug effects ; Oxidative Stress/drug effects ; Reactive Oxygen Species/metabolism |
| مستخلص: | Cobalt (III) Schiff base complexes are of attraction in the context of their potential application in cancer therapy. The aim of this study has been to find the mechanism of action of cobalt (III) Schiff base complexes 1 and 2, the synthesis and characterization of which have already been reported, in inhibiting growth of human breast cancer cell MCF-7 and lung cancer cell A549. The already proclaimed anti-proliferative effect of the cobalt complexes was ascertained using MTT cytotoxicity assay. More assays such as Acridine orange & Ethidium bromide staining, AnnexinV-Cy3 staining, Hoechst staining, comet assay, and Reactive Oxygen Species (ROS) assay- all supported the cytotoxic property of the complexes. Moreover, the expression levels of mRNA of pro- and antiapoptotic genes also supported the effectiveness of cobalt complexes by modifying the ratio of Bax: Bcl-2. In addition, the cobalt complexes induced apoptosis in MCF- 7 and A549 cells through modulation of pro-apoptotic, anti-apoptotic, and ROS modulatory gene expressions. The present study validates the scientific evidence for antiproliferative efficacy of cobalt complexes against MCF-7 and A549 cells. Thus, this study takes cobalt complexes 1 and 2 to a step higher towards their use as anticancer agents. (Copyright © 2021. Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Apoptosis; Cobalt (III) Schiff base complexes; DNA damage; Immunofluorescence; ROS; RT-PCR |
| المشرفين على المادة: | 0 (Antineoplastic Agents) 0 (Coordination Complexes) 0 (Reactive Oxygen Species) 0 (Schiff Bases) 3G0H8C9362 (Cobalt) |
| تواريخ الأحداث: | Date Created: 20210622 Date Completed: 20220107 Latest Revision: 20220107 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.tiv.2021.105201 |
| PMID: | 34157415 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-3177 |
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| DOI: | 10.1016/j.tiv.2021.105201 |