A SARS-CoV-2 spike ferritin nanoparticle vaccine protects against heterologous challenge with B.1.1.7 and B.1.351 virus variants in Syrian golden hamsters.
| العنوان: | A SARS-CoV-2 spike ferritin nanoparticle vaccine protects against heterologous challenge with B.1.1.7 and B.1.351 virus variants in Syrian golden hamsters. |
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| المؤلفون: | Wuertz KM; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Barkei EK; Veterinary Pathology Division, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Chen WH; Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Martinez EJ; Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Lakhal-Naouar I; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA.; Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Jagodzinski LL; Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Paquin-Proulx D; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Gromowski GD; Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Swafford I; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Ganesh A; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Oak Ridge Institute of Science and Education, Oak Ridge, Tennessee USA., Dong M; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Zeng X; Pathology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland USA., Thomas PV; Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Sankhala RS; Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Hajduczki A; Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Peterson CE; Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Kuklis C; Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Soman S; Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Wieczorek L; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Zemil M; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Anderson A; Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Oak Ridge Institute of Science and Education, Oak Ridge, Tennessee USA., Darden J; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA.; Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Hernandez H; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA.; Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Grove H; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA.; Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Dussupt V; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Hack H; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA.; Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., de la Barrera R; Pilot Bioproduction Facility, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Zarling S; Pilot Bioproduction Facility, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Wood JF; Pilot Bioproduction Facility, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Froude JW; Pilot Bioproduction Facility, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Gagne M; Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland USA., Henry AR; Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland USA., Mokhtari EB; Virus Persistence and Dynamics Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland USA., Mudvari P; Virus Persistence and Dynamics Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland USA., Krebs SJ; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Pekosz AS; W. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland USA., Currier JR; Virus Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Kar S; BioQual, Inc., Rockville, Maryland USA., Porto M; BioQual, Inc., Rockville, Maryland USA., Winn A; BioQual, Inc., Rockville, Maryland USA., Radzyminski K; BioQual, Inc., Rockville, Maryland USA., Lewis MG; BioQual, Inc., Rockville, Maryland USA., Vasan S; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA., Suthar M; Emory Vaccine Center, Department of Pediatrics, Emory School of Medicine, Atlanta, Georgia USA., Polonis VR; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Matyas GR; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Boritz EA; Virus Persistence and Dynamics Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland USA., Douek DC; Human Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland USA., Seder RA; Cellular Immunology Section, Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland USA., Daye SP; One Health Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Rao M; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Peel SA; Diagnostics Countermeasures Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA., Joyce MG; Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA.; These authors contributed equally., Bolton DL; U.S. Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland USA.; These authors contributed equally., Michael NL; Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; These authors contributed equally., Modjarrad K; Emerging Infectious Diseases Branch, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, Maryland USA.; These authors contributed equally. |
| المصدر: | BioRxiv : the preprint server for biology [bioRxiv] 2021 Jun 16. Date of Electronic Publication: 2021 Jun 16. |
| نوع المنشور: | Preprint |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: | The emergence of SARS-CoV-2 variants of concern (VOC) requires adequate coverage of vaccine protection. We evaluated whether a spike ferritin nanoparticle vaccine (SpFN), adjuvanted with the Army Liposomal Formulation QS21 (ALFQ), conferred protection against the B.1.1.7 and B.1.351 VOCs in Syrian golden hamsters. SpFN-ALFQ was administered as either single or double-vaccination (0 and 4 week) regimens, using a high (10 μg) or low (0.2 μg) immunogen dose. Animals were intranasally challenged at week 11. Binding antibody responses were comparable between high- and low-dose groups. Neutralizing antibody titers were equivalent against WA1, B.1.1.7, and B.1.351 variants following two high dose two vaccinations. SpFN-ALFQ vaccination protected against SARS-CoV-2-induced disease and viral replication following intranasal B.1.1.7 or B.1.351 challenge, as evidenced by reduced weight loss, lung pathology, and lung and nasal turbinate viral burden. These data support the development of SpFN-ALFQ as a broadly protective, next-generation SARS-CoV-2 vaccine. |
| التعليقات: | Update in: NPJ Vaccines. 2021 Oct 28;6(1):129. (PMID: 34711815) |
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| تواريخ الأحداث: | Date Created: 20210623 Latest Revision: 20231107 |
| رمز التحديث: | 20231107 |
| مُعرف محوري في PubMed: | PMC8219092 |
| DOI: | 10.1101/2021.06.16.448525 |
| PMID: | 34159328 |
| قاعدة البيانات: | MEDLINE |
| DOI: | 10.1101/2021.06.16.448525 |
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