دورية أكاديمية
Galectin-3 regulates proinflammatory cytokine function and favours Brucella abortus chronic replication in macrophages and mice.
| العنوان: | Galectin-3 regulates proinflammatory cytokine function and favours Brucella abortus chronic replication in macrophages and mice. |
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| المؤلفون: | Tana FL; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Guimarães ES; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Cerqueira DM; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Campos PC; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Gomes MTR; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Marinho FV; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Oliveira SC; Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.; Departamento de Genética, Ecologia e Evolução, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.; Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT-DT), CNPq MCT, Salvador, Brazil. |
| المصدر: | Cellular microbiology [Cell Microbiol] 2021 Oct; Vol. 23 (10), pp. e13375. Date of Electronic Publication: 2021 Jul 02. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Hindawi Country of Publication: India NLM ID: 100883691 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1462-5822 (Electronic) Linking ISSN: 14625814 NLM ISO Abbreviation: Cell Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2022- : Mumbai : Hindawi Original Publication: Oxford : Wiley-Blackwell, c1999- |
| مواضيع طبية MeSH: | Brucella abortus* , Brucellosis*, Galectin 3/*metabolism, Animals ; Cytokines ; Galectin 3/genetics ; Macrophages ; Mice ; Mice, Knockout |
| مستخلص: | In this study, we provide evidence that galectin-3 (Gal-3) plays an important role in Brucella abortus infection. Our results showed increased Gal-3 expression and secretion in B. abortus infected macrophages and mice. Additionally, our findings indicate that Gal-3 is dispensable for Brucella-containing vacuoles disruption, inflammasome activation and pyroptosis. On the other hand, we observed that Brucella-induced Gal-3 expression is crucial for induction of molecules associated to type I IFN signalling pathway, such as IFN-β: Interferon beta (IFN-β), C-X-C motif chemokine ligand 10 (CXCL10) and guanylate-binding proteins. Gal-3 KO macrophages showed reduced bacterial numbers compared to wild-type cells, suggesting that Gal-3 facilitates bacterial replication in vitro. Moreover, priming Gal-3 KO cells with IFN-β favoured B. abortus survival in macrophages. Additionally, we also observed that Gal-3 KO mice are more resistant to B. abortus infection and these animals showed elevated production of proinflammatory cytokines when compared to control mice. Finally, we observed an increased recruitment of macrophages, dendritic cells and neutrophils in spleens of Gal-3 KO mice compared to wild-type animals. In conclusion, this study demonstrated that Brucella-induced Gal-3 is detrimental to host and this molecule is implicated in inhibition of recruitment and activation of immune cells, which promotes B. abortus spread and aggravates the infection. TAKE AWAYS: Brucella abortus infection upregulates galectin-3 expression Galectin-3 regulates guanylate-binding proteins expression but is not required for Brucella-containing vacuole disruption Galectin-3 modulates proinflammatory cytokine production during bacterial infection Galectin-3 favours Brucella replication. (© 2021 John Wiley & Sons Ltd.) |
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| معلومات مُعتمدة: | APQ-01945-17 Fundação de Amparo à Pesquisa do Estado de Minas Gerais; 465229/2014-0 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 406883/2018-1 Conselho Nacional de Desenvolvimento Científico e Tecnológico; REDE-00140-16 Fundação de Amparo à Pesquisa do Estado de Minas Gerais; R01 AI116453 United States AI NIAID NIH HHS; 303044/2020-9 Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| فهرسة مساهمة: | Keywords: Brucella abortus; galectin-3; guanylate-binding protein; inflammasome; type I IFN; vacuole |
| المشرفين على المادة: | 0 (Cytokines) 0 (Galectin 3) 0 (Lgals3 protein, mouse) |
| تواريخ الأحداث: | Date Created: 20210625 Date Completed: 20220113 Latest Revision: 20221003 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8635083 |
| DOI: | 10.1111/cmi.13375 |
| PMID: | 34169616 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1462-5822 |
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| DOI: | 10.1111/cmi.13375 |