دورية أكاديمية
أعرض في EDS

Lung microbiota predict invasive pulmonary aspergillosis and its outcome in immunocompromised patients.

التفاصيل البيبلوغرافية
العنوان: Lung microbiota predict invasive pulmonary aspergillosis and its outcome in immunocompromised patients.
المؤلفون: Hérivaux A; Life and Health Sciences Research Institute (ICVS), University of Minho, Braga, Portugal.; ICVS/3B's - PT Government Associate Laboratory, Guimarães/Braga, Portugal., Willis JR; Barcelona Supercomputing Centre (BSC-CNS), Barcelona, Spain.; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain., Mercier T; Department of Hematology, University Hospitals Leuven, Leuven, Belgium.; Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium., Lagrou K; Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium.; Clinical Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium., Gonçalves SM; Life and Health Sciences Research Institute (ICVS), University of Minho, Braga, Portugal.; ICVS/3B's - PT Government Associate Laboratory, Guimarães/Braga, Portugal., Gonçales RA; Life and Health Sciences Research Institute (ICVS), University of Minho, Braga, Portugal.; ICVS/3B's - PT Government Associate Laboratory, Guimarães/Braga, Portugal., Maertens J; Department of Hematology, University Hospitals Leuven, Leuven, Belgium.; Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium., Carvalho A; Life and Health Sciences Research Institute (ICVS), University of Minho, Braga, Portugal.; ICVS/3B's - PT Government Associate Laboratory, Guimarães/Braga, Portugal., Gabaldón T; Barcelona Supercomputing Centre (BSC-CNS), Barcelona, Spain cristinacunha@med.uminho.pt toni.gabaldon.bcn@gmail.com.; Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain.; Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain., Cunha C; Life and Health Sciences Research Institute (ICVS), University of Minho, Braga, Portugal cristinacunha@med.uminho.pt toni.gabaldon.bcn@gmail.com.; ICVS/3B's - PT Government Associate Laboratory, Guimarães/Braga, Portugal.
المصدر: Thorax [Thorax] 2022 Mar; Vol. 77 (3), pp. 283-291. Date of Electronic Publication: 2021 Jun 25.
نوع المنشور: Journal Article; Observational Study; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: British Medical Assn Country of Publication: England NLM ID: 0417353 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1468-3296 (Electronic) Linking ISSN: 00406376 NLM ISO Abbreviation: Thorax Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : British Medical Assn.
مواضيع طبية MeSH: Invasive Pulmonary Aspergillosis*/diagnosis , Microbiota*/genetics, Bronchoalveolar Lavage Fluid/microbiology ; Humans ; Immunocompromised Host ; Lung/microbiology
مستخلص: Rationale: Recent studies have revealed that the lung microbiota of critically ill patients is altered and predicts clinical outcomes. The incidence of invasive fungal infections, namely, invasive pulmonary aspergillosis (IPA), in immunocompromised patients is increasing, but the clinical significance of variations in lung bacterial communities is unknown.
Objectives: To define the contribution of the lung microbiota to the development and course of IPA.
Methods and Measurements: We performed an observational cohort study to characterise the lung microbiota in 104 immunocompromised patients using bacterial 16S ribosomal RNA gene sequencing on bronchoalveolar lavage samples sampled on clinical suspicion of infection. Associations between lung dysbiosis in IPA and pulmonary immunity were evaluated by quantifying alveolar cytokines and chemokines and immune cells. The contribution of microbial signatures to patient outcome was assessed by estimating overall survival.
Main Results: Patients diagnosed with IPA displayed a decreased alpha diversity, driven by a markedly increased abundance of the Staphylococcus , Escherichia , Paraclostridium and Finegoldia genera and a decreased proportion of the Prevotella and Veillonella genera. The overall composition of the lung microbiome was influenced by the neutrophil counts and associated with differential levels of alveolar cytokines. Importantly, the degree of bacterial diversity at the onset of IPA predicted the survival of infected patients.
Conclusions: Our results reveal the lung microbiota as an understudied source of clinical variation in patients at risk of IPA and highlight its potential as a diagnostic and therapeutic target in the context of respiratory fungal diseases.
Competing Interests: Competing interests: KL reports personal fees and non-financial support from Pfizer and MSD and personal fees from SMB Laboratoires, Gilead and FUJIFILM Wako, outside the submitted work. JM reports personal fees and non-financial support from MSD, Cidara, F2G and Scynexis; grants, personal fees and non-financial support from Pfizer and Gilead; and non-financial support from Bio-Rad, outside the submitted work.
(© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
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فهرسة مساهمة: Keywords: aspergillus lung disease; critical care; opportunist lung infections; respiratory infection
تواريخ الأحداث: Date Created: 20210626 Date Completed: 20220324 Latest Revision: 20220324
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8867272
DOI: 10.1136/thoraxjnl-2020-216179
PMID: 34172558
قاعدة البيانات: MEDLINE
الوصف
تدمد:1468-3296
DOI:10.1136/thoraxjnl-2020-216179