دورية أكاديمية
Transcriptomic Changes Within Human Bone Marrow After Severe Trauma.
| العنوان: | Transcriptomic Changes Within Human Bone Marrow After Severe Trauma. |
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| المؤلفون: | Kelly LS; Department of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida., Apple CG; Department of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida., Darden DB; Department of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida., Kannan KB; Department of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida., Pons EE; Department of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida., Fenner BP; Department of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida., Parvataneni HK; Department of Orthopaedic Surgery, University of Florida College of Medicine, Gainesville, Florida., Hagen JE; Department of Orthopaedic Surgery, University of Florida College of Medicine, Gainesville, Florida., Brakenridge SC; Department of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida., Efron PA; Department of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida., Mohr AM; Department of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida. |
| المصدر: | Shock (Augusta, Ga.) [Shock] 2022 Jan 01; Vol. 57 (1), pp. 24-30. |
| نوع المنشور: | Journal Article; Observational Study; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 9421564 Publication Model: Print Cited Medium: Internet ISSN: 1540-0514 (Electronic) Linking ISSN: 10732322 NLM ISO Abbreviation: Shock Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2002- : Philadelphia : Lippincott Williams & Wilkins Original Publication: Augusta, GA : BioMedical Press, [1994- |
| مواضيع طبية MeSH: | Femoral Fractures* , Hip Fractures*, Bone Marrow/*metabolism , RNA, Messenger/*metabolism, Adult ; Arthroplasty, Replacement, Hip ; Case-Control Studies ; Cation Transport Proteins/genetics ; Cation Transport Proteins/metabolism ; Down-Regulation ; Hepcidins/genetics ; Hepcidins/metabolism ; Humans ; Interleukins/genetics ; Interleukins/metabolism ; Lymphotoxin-alpha/genetics ; Lymphotoxin-alpha/metabolism ; Male ; Middle Aged ; Prospective Studies ; Receptors, Interleukin-6/genetics ; Receptors, Interleukin-6/metabolism ; Receptors, Transforming Growth Factor beta/genetics ; Receptors, Transforming Growth Factor beta/metabolism ; Toll-Like Receptor 4/genetics ; Toll-Like Receptor 4/metabolism ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/metabolism ; Up-Regulation |
| مستخلص: | Background: Severe trauma is associated with severe systemic inflammation and neuroendocrine activation that is associated with erythroid progenitor growth suppression and refractory anemia. Although distinct transcriptional profiles have been detected in numerous tissue types after trauma, no study has yet characterized this within the bone marrow. This study sought to identify a unique bone marrow transcriptomic response following trauma. Methods: In a prospective observational cohort study, bone marrow was obtained from severely injured trauma patients with a hip or femur fracture (n = 52), elective hip replacement patients (n = 33), and healthy controls (n = 11). RNA was isolated from bone marrow using a Purelink RNA mini kit. Direct quantification of mRNA copies was performed by NanoString Technologies on a custom gene panel. Results: Trauma patients displayed an upregulation of genes encoding receptors known to have inhibitory downstream effects on erythropoiesis, including ferroportin, interleukin-6 (IL-6) receptor, transforming growth factor-beta (TGF-β) receptor, and IL-10, as well as genes involved in innate immunity including toll-like receptor 4 (TLR4)-mediated signaling factors. In contrast, hip replacement patients had downregulated transcription of IL-1β, IL-6, TGF-β, tumor necrosis factor alpha, and the HAMP gene with no change in TLR4-mediated signaling factors. Conclusions: A unique transcriptomic response within the bone marrow was identified following severe trauma compared to elective hip replacement. These transcriptomic differences were related to the innate immune response as well as known inhibitors of erythropoiesis. Although confined to just one time point, this differential transcriptional response may be linked to refractory anemia and inflammation after injury. Competing Interests: The authors report no conflicts of interest. (Copyright © 2021 by the Shock Society.) |
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| معلومات مُعتمدة: | R01 GM113945 United States GM NIGMS NIH HHS; T32 GM008721 United States GM NIGMS NIH HHS; P30 AG028740 United States AG NIA NIH HHS; P50 GM111152 United States GM NIGMS NIH HHS; R01 GM105893 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (Cation Transport Proteins) 0 (HAMP protein, human) 0 (Hepcidins) 0 (Interleukins) 0 (Lymphotoxin-alpha) 0 (RNA, Messenger) 0 (Receptors, Interleukin-6) 0 (Receptors, Transforming Growth Factor beta) 0 (Toll-Like Receptor 4) 0 (Tumor Necrosis Factor-alpha) 0 (metal transporting protein 1) |
| تواريخ الأحداث: | Date Created: 20210626 Date Completed: 20220301 Latest Revision: 20230824 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8678139 |
| DOI: | 10.1097/SHK.0000000000001826 |
| PMID: | 34172608 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1540-0514 |
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| DOI: | 10.1097/SHK.0000000000001826 |