دورية أكاديمية
Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells.
| العنوان: | Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells. |
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| المؤلفون: | Souza KM; Universidade do Estado de Santa Catarina, Departamento de Produção Animal e Alimentos, Lages, SC, Brasil., Mendes IC; Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Belo Horizonte, MG, Brasil., Dall'Igna DM; Universidade do Estado de Santa Catarina, Departamento de Produção Animal e Alimentos, Lages, SC, Brasil.; Universidade do Planalto Catarinense, Lages, SC, Brasil., Repolês BM; Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Belo Horizonte, MG, Brasil., Resende BC; Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Belo Horizonte, MG, Brasil., Moreira RS; Universidade do Estado de Santa Catarina, Departamento de Produção Animal e Alimentos, Lages, SC, Brasil.; Instituto Federal de Santa Catarina, Departamento de Ensino, Pesquisa e Extensão, Lages, SC, Brasil., Miletti LC; Universidade do Estado de Santa Catarina, Departamento de Produção Animal e Alimentos, Lages, SC, Brasil., Machado CR; Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Bioquímica e Imunologia, Belo Horizonte, MG, Brasil., Vogel CIG; Universidade do Estado de Santa Catarina, Departamento de Produção Animal e Alimentos, Lages, SC, Brasil. |
| المصدر: | Brazilian journal of biology = Revista brasleira de biologia [Braz J Biol] 2021 Jun 28; Vol. 83, pp. e243910. Date of Electronic Publication: 2021 Jun 28 (Print Publication: 2021). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: International Institute of Ecology Country of Publication: Brazil NLM ID: 101129542 Publication Model: eCollection Cited Medium: Internet ISSN: 1678-4375 (Electronic) Linking ISSN: 15196984 NLM ISO Abbreviation: Braz J Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: São Carlos, SP [Brazil] : International Institute of Ecology, [2000]- |
| مواضيع طبية MeSH: | Trypanosoma cruzi*/genetics , Xeroderma Pigmentosum*, Animals ; Computational Biology ; DNA Damage/genetics ; DNA Repair/genetics ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Humans |
| مستخلص: | Nucleotide excision repair (NER) acts repairing damages in DNA, such as lesions caused by cisplatin. Xeroderma Pigmentosum complementation group C (XPC) protein is involved in recognition of global genome DNA damages during NER (GG-NER) and it has been studied in different organisms due to its importance in other cellular processes. In this work, we studied NER proteins in Trypanosoma cruzi and Trypanosoma evansi, parasites of humans and animals respectively. We performed three-dimensional models of XPC proteins from T. cruzi and T. evansi and observed few structural differences between these proteins. In our tests, insertion of XPC gene from T. evansi (TevXPC) in T. cruzi resulted in slower cell growth under normal conditions. After cisplatin treatment, T. cruzi overexpressing its own XPC gene (TcXPC) was able to recover cell division rates faster than T. cruzi expressing TevXPC gene. Based on these tests, it is suggested that TevXPC (being an exogenous protein in T. cruzi) interferes negatively in cellular processes where TcXPC (the endogenous protein) is involved. This probably occurred due interaction of TevXPC with some endogenous molecules or proteins from T.cruzi but incapacity of interaction with others. This reinforces the importance of correctly XPC functioning within the cell. |
| المشرفين على المادة: | 0 (DNA-Binding Proteins) 156533-34-5 (XPC protein, human) |
| تواريخ الأحداث: | Date Created: 20210630 Date Completed: 20210702 Latest Revision: 20210702 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1590/1519-6984.243910 |
| PMID: | 34190757 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1678-4375 |
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| DOI: | 10.1590/1519-6984.243910 |