دورية أكاديمية
Testicular pathology in fatal COVID-19: A descriptive autopsy study.
| العنوان: | Testicular pathology in fatal COVID-19: A descriptive autopsy study. |
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| المؤلفون: | Duarte-Neto AN; Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.; Instituto Adolfo Lutz, São Paulo, Brazil., Teixeira TA; Departamento de Cirurgia, Disciplina de Urologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Caldini EG; Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Kanamura CT; Instituto Adolfo Lutz, São Paulo, Brazil., Gomes-Gouvêa MS; Departamento de Gastroenterologia (LIM-07), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Dos Santos ABG; Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Monteiro RAA; Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Pinho JRR; Departamento de Gastroenterologia (LIM-07), Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Mauad T; Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., da Silva LFF; Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.; Serviço de Verificação de Óbitos da Capital, Universidade de São Paulo, São Paulo, Brazil., Saldiva PHN; Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Dolhnikoff M; Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Leite KRM; Departamento de Cirurgia, Disciplina de Urologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Hallak J; Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.; Departamento de Cirurgia, Disciplina de Urologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil. |
| المصدر: | Andrology [Andrology] 2022 Jan; Vol. 10 (1), pp. 13-23. Date of Electronic Publication: 2021 Jul 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101585129 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2047-2927 (Electronic) Linking ISSN: 20472919 NLM ISO Abbreviation: Andrology Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford : Wiley-Blackwell, 2013- |
| مواضيع طبية MeSH: | COVID-19/*complications , Orchitis/*pathology , Orchitis/*virology , Testis/*pathology, Adult ; Aged ; Aged, 80 and over ; Autopsy ; Humans ; Male ; Middle Aged ; SARS-CoV-2 |
| مستخلص: | Background: Multi-organ damage is a common feature of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, going beyond the initially observed severe pneumonia. Evidence that the testis is also compromised is growing. Objective: To describe the pathological findings in testes from fatal cases of COVID-19, including the detection of viral particles and antigens, and inflammatory cell subsets. Materials and Methods: Postmortem testicular samples were obtained by percutaneous puncture from 11 deceased men and examined by reverse-transcription polymerase chain reaction (RT-PCR) for RNA detection and by light and electron microscopy (EM) for SARS-CoV-2. Immunohistochemistry (IHC) for the SARS-CoV-2 N-protein and lymphocytic and histiocytic markers was also performed. Results: Eight patients had mild interstitial orchitis, composed mainly of CD68+ and TCD8+ cells. Fibrin thrombi were detected in five cases. All cases presented congestion, interstitial edema, thickening of the tubular basal membrane, decreased Leydig and Sertoli cells with reduced spermatogenesis, and strong expression of vascular cell adhesion molecule (VCAM) in vessels. IHC detected SARS-Cov-2 antigen in Leydig cells, Sertoli cells, spermatogonia, and fibroblasts in all cases. EM detected viral particles in the cytoplasm of fibroblasts, endothelium, Sertoli and Leydig cells, spermatids, and epithelial cells of the rete testis in four cases, while RT-PCR detected SARS-CoV-2 RNA in three cases. Discussion and Conclusion: The COVID-19-associated testicular lesion revealed a combination of orchitis, vascular changes, basal membrane thickening, Leydig and Sertoli cell scarcity, and reduced spermatogenesis associated with SARS-CoV-2 local infection that may impair hormonal function and fertility in men. (© 2021 American Society of Andrology and European Academy of Andrology.) |
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| فهرسة مساهمة: | Keywords: COVID-19; Leydig cell; SARS-CoV-2; autopsy; orchitis; testis |
| تواريخ الأحداث: | Date Created: 20210701 Date Completed: 20220106 Latest Revision: 20221005 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC8444746 |
| DOI: | 10.1111/andr.13073 |
| PMID: | 34196475 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2047-2927 |
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| DOI: | 10.1111/andr.13073 |