دورية أكاديمية

Molecular Detection of Trypanosoma kaiowa in Tabanus triangulum (Diptera: Tabanidae) from the Coastal Plain of Rio Grande do Sul, Southern Brazil.

التفاصيل البيبلوغرافية
العنوان: Molecular Detection of Trypanosoma kaiowa in Tabanus triangulum (Diptera: Tabanidae) from the Coastal Plain of Rio Grande do Sul, Southern Brazil.
المؤلفون: Rodrigues GD; Ecology of Parasites and Vectors Group, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil. gratidrodrigues.gdr@gmail.com., Blodorn E; Programa de Pós-Graduação em Biotecnologia (PPBB), Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil., Zafalon-Silva Â; Departamento de Zoologia, Universidade Federal do Paraná, Curitiba, Brazil., Domingues W; Programa de Pós-Graduação em Biotecnologia (PPBB), Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil., Marques R; Ecology of Parasites and Vectors Group, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil.; Programa de Pós-Graduação em Microbiologia e Parasitologia (PPGMP), Universidade Federal de Pelotas, Rio Grande do Sul, Brazil., Krolow TK; Ecology of Parasites and Vectors Group, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil.; Programa de Pós-Graduação em Biodiversidade, Ecologia e Conservação (PPGBEC), Universidade Federal do Tocantins, Tocantins, Brazil., Greif G; Laboratório de Interações Hospedeiro-Patógeno/Unidade de Biologia Molecular, Instituto Pasteur de Montevideo, Montevideo, Uruguay., Campos VF; Programa de Pós-Graduação em Biotecnologia (PPBB), Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil., Krüger RF; Ecology of Parasites and Vectors Group, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil.; Programa de Pós-Graduação em Microbiologia e Parasitologia (PPGMP), Universidade Federal de Pelotas, Rio Grande do Sul, Brazil.; Programa de Pós-Graduação em Biodiversidade, Ecologia e Conservação (PPGBEC), Universidade Federal do Tocantins, Tocantins, Brazil.
المصدر: Acta parasitologica [Acta Parasitol] 2022 Mar; Vol. 67 (1), pp. 518-522. Date of Electronic Publication: 2021 Jul 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 9301947 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1896-1851 (Electronic) Linking ISSN: 12302821 NLM ISO Abbreviation: Acta Parasitol Subsets: MEDLINE
أسماء مطبوعة: Publication: 2019- : Cham : Springer International Publishing
Original Publication: Warszawa : Witold Stefanski Institute Of Parasitology
مواضيع طبية MeSH: Diptera* , Trypanosoma*/genetics, Animals ; Brazil ; Phylogeny ; Sequence Analysis, DNA
مستخلص: Purpose: The species of the genus Trypanosoma are carried and transmitted by horseflies parasitizing a high diversity of vertebrates. In the Coastal Plain of Rio Grande do Sul, southern Brazil, Tabanus triangulum is the most abundant species and, similarly to the other species of horseflies, there is little knowledge about its vector competence. Therefore, this study aimed to screen the field-collected T. triangulum for the presence of Trypanosoma, to estimate infectivity.
Methods: Horseflies were sampled by the Malaise trap in the forest fragments at the coastal plain and DNA was extracted from whole body flies. The Polymerase Chain Reaction was performed.
Results: Horseflies presented amplification of 18S ribosomal gene-specific of Trypanosoma species. DNA sequencing and phylogenetic analysis positioned the strains in the Kaiowa clade with Trypanosoma kaiowa, associated with the crocodilian clade of Trypanosoma.
Conclusion: This study represents the first report of the presence of the Tr. kaiowa in T. triangulum and the expansion of the parasite's range further south in South America.
(© 2021. Witold Stefański Institute of Parasitology, Polish Academy of Sciences.)
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معلومات مُعتمدة: 128699/2019-1 CNPQ; 308908/2016-3 CNPQ
فهرسة مساهمة: Keywords: Crocodilians; Pampa biome; Tabanidae; Tabanus; Trypanosome
تواريخ الأحداث: Date Created: 20210701 Date Completed: 20220323 Latest Revision: 20220323
رمز التحديث: 20221213
DOI: 10.1007/s11686-021-00440-1
PMID: 34196921
قاعدة البيانات: MEDLINE
الوصف
تدمد:1896-1851
DOI:10.1007/s11686-021-00440-1