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Mapping Salivary Proteases in Sjögren's Syndrome Patients Reveals Overexpression of Dipeptidyl Peptidase-4/CD26.

التفاصيل البيبلوغرافية
العنوان: Mapping Salivary Proteases in Sjögren's Syndrome Patients Reveals Overexpression of Dipeptidyl Peptidase-4/CD26.
المؤلفون: Garreto L; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, Brazil., Charneau S; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, Brazil., Mandacaru SC; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, Brazil., Nóbrega OT; Faculty of Ceilândia, University of Brasília, Brasília, Brazil., Motta FN; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, Brazil.; Faculty of Ceilândia, University of Brasília, Brasília, Brazil., de Araújo CN; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, Brazil.; Faculty of Ceilândia, University of Brasília, Brasília, Brazil., Tonet AC; Laboratory of Immune Gerontology, Catholic University of Brasília, Brasília, Brazil., Modesto FMB; Department of Dentistry, Paulista University, Brasília, Brazil., Paula LM; Laboratory of Oral Histopathology, Department of Odontology, Health Sciences Faculty, University of Brasília, Brasília, Brazil., de Sousa MV; Laboratory of Protein Chemistry and Biochemistry, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, Brazil., Santana JM; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, Brazil., Acevedo AC; Laboratory of Oral Histopathology, Department of Odontology, Health Sciences Faculty, University of Brasília, Brasília, Brazil., Bastos IMD; Pathogen-Host Interface Laboratory, Department of Cell Biology, Institute of Biology, University of Brasília, Brasília, Brazil.
المصدر: Frontiers in immunology [Front Immunol] 2021 Jun 17; Vol. 12, pp. 686480. Date of Electronic Publication: 2021 Jun 17 (Print Publication: 2021).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101560960 Publication Model: eCollection Cited Medium: Internet ISSN: 1664-3224 (Electronic) Linking ISSN: 16643224 NLM ISO Abbreviation: Front Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Lausanne : Frontiers Research Foundation]
مواضيع طبية MeSH: Dipeptidyl Peptidase 4/*metabolism , Peptide Hydrolases/*analysis , Proteomics/*methods , Saliva/*metabolism , Sjogren's Syndrome/*metabolism, Adult ; Biomarkers/metabolism ; Case-Control Studies ; Cathepsin G ; Female ; Humans ; Leukocyte Elastase ; Male ; Mass Spectrometry ; Middle Aged ; Serine Endopeptidases ; Signal Transduction ; Sjogren's Syndrome/diagnosis
مستخلص: Sjögren's Syndrome (SS) is an autoimmune exocrinopathy characterized by the progressive damage of salivary and lacrimal glands associated with lymphocytic infiltration. Identifying new non-invasive biomarkers for SS diagnosis remains a challenge, and alterations in saliva composition reported in patients turn this fluid into a source of potential biomarkers. Among these, proteases are promising candidates since they are involved in several key physio-pathological processes. This study evaluated differentially expressed proteases in SS individuals' saliva using synthetic fluorogenic substrates, zymography, ELISA, and proteomic approaches. Here we reported, for the first time, increased activity of the serine protease dipeptidyl peptidase-4/CD26 (DPP4/CD26) in pSS saliva, the expression level of which was corroborated by ELISA assay. Gelatin zymograms showed that metalloproteinase proteolytic band profiles differed significantly in intensity between control and SS groups. Focusing on matrix metalloproteinase-9 (MMP9) expression, an increased tendency in pSS saliva (p = 0.0527) was observed compared to the control group. Samples of control, pSS, and sSS were analyzed by mass spectrometry to reveal a general panorama of proteases in saliva. Forty-eight protein groups of proteases were identified, among which were the serine proteases cathepsin G (CTSG), neutrophil elastase (ELANE), myeloblastin (PRTN3), MMP9 and several protease inhibitors. This work paves the way for proteases to be explored in the future as biomarkers, emphasizing DPP4 by its association in several autoimmune and inflammatory diseases. Besides its proteolytic role, DPP4/CD26 acts as a cell surface receptor, signal transduction mediator, adhesion and costimulatory protein involved in T lymphocytes activation.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Garreto, Charneau, Mandacaru, Nóbrega, Motta, de Araújo, Tonet, Modesto, Paula, de Sousa, Santana, Acevedo and Bastos.)
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فهرسة مساهمة: Keywords: DPP4/CD26; Sjögren’s syndrome; protease activity; protease inhibitors; proteome; saliva
المشرفين على المادة: 0 (Biomarkers)
EC 3.4.- (Peptide Hydrolases)
EC 3.4.14.5 (DPP4 protein, human)
EC 3.4.14.5 (Dipeptidyl Peptidase 4)
EC 3.4.21.- (Serine Endopeptidases)
EC 3.4.21.20 (Cathepsin G)
EC 3.4.21.37 (Leukocyte Elastase)
تواريخ الأحداث: Date Created: 20210705 Date Completed: 20211028 Latest Revision: 20240402
رمز التحديث: 20240402
مُعرف محوري في PubMed: PMC8247581
DOI: 10.3389/fimmu.2021.686480
PMID: 34220840
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-3224
DOI:10.3389/fimmu.2021.686480