دورية أكاديمية
أعرض في EDS

What's under the Christmas Tree? A Soil Sulfur Amendment Lowers Soil pH and Alters Fir Tree Rhizosphere Bacterial and Eukaryotic Communities, Their Interactions, and Functional Traits.

التفاصيل البيبلوغرافية
العنوان: What's under the Christmas Tree? A Soil Sulfur Amendment Lowers Soil pH and Alters Fir Tree Rhizosphere Bacterial and Eukaryotic Communities, Their Interactions, and Functional Traits.
المؤلفون: Steven B; Department of Environmental Sciences, Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA., LaReau JC; Department of Environmental Sciences, Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA., Taerum SJ; Department of Plant Pathology and Ecology, Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA., Zuverza-Mena N; Department of Analytical Chemistry, Connecticut Agricultural Experiment Station, New Haven, Connecticut, USA., Cowles RS; Valley Laboratory, Connecticut Agricultural Experiment Station, Windsor, Connecticut, USA.
المصدر: Microbiology spectrum [Microbiol Spectr] 2021 Sep 03; Vol. 9 (1), pp. e0016621. Date of Electronic Publication: 2021 Jul 07.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: ASM Press Country of Publication: United States NLM ID: 101634614 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2165-0497 (Electronic) Linking ISSN: 21650497 NLM ISO Abbreviation: Microbiol Spectr Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, DC : ASM Press, 2013-
مواضيع طبية MeSH: Soil Microbiology*, Abies/*growth & development , Bacteria/*isolation & purification , Eukaryota/*isolation & purification , Soil/*chemistry , Soil/*parasitology , Sulfur/*metabolism, Abies/microbiology ; Bacteria/classification ; Bacteria/genetics ; Biodiversity ; Eukaryota/classification ; Eukaryota/genetics ; Hydrogen-Ion Concentration ; Metagenome ; Rhizosphere ; Trees/growth & development ; Trees/microbiology
مستخلص: In this study, we describe the legacy effects of a soil sulfur amendment experiment performed 6 years prior and the resulting alterations to the rhizosphere communities of fir trees on a Christmas tree plantation. The pH of bulk soil was ∼1.4 pH units lower than that of untreated soils and was associated with reduced Ca, Mg, and organic matter contents. Similarly, root chemistry differed due to the treatment, with roots in sulfur-amended soils showing significantly higher Al, Mn, and Zn contents and reduced levels of B and Ca. 16S rRNA and 18S rRNA gene sequencing was pursued to characterize the bacterial/archaeal and eukaryotic communities in the rhizosphere soils. The treatment induced dramatic and significant changes in the microbial populations, with thousands of 16S rRNA gene sequence variants and hundreds of 18S rRNA gene variants being significantly different in relative abundances between the treatments. Additionally, co-occurrence networks showed that bacterial and eukaryotic interactions, network topology, and hub taxa were significantly different when constructed from the control and treated soil 16S and 18S rRNA gene amplicon libraries. Metagenome sequencing identified several genes related to transport proteins that differentiated the functional potentials of the communities between treatments, pointing to physiological adaptations in the microbial communities for living at altered pH. These data show that a legacy of soil acidification increased the heterogeneity of the soil communities as well as decreasing taxon connections, pointing to a state of ecosystem instability that has potentially persisted for 6 years. IMPORTANCE We used sulfur incorporation to investigate the legacy effects of lowered soil pH on the bacterial and eukaryotic populations in the rhizosphere of Christmas trees. Acidification of the soils drove alterations of fir tree root chemistry and large shifts in the taxonomic and functional compositions of the communities. These data demonstrate that soil pH influences are manifest across all organisms inhabiting the soil, from the host plant to the microorganisms inhabiting the rhizosphere soils. Thus, this study highlights the long-lasting influence of altering soil pH on soil and plant health as well as the status of the microbiome.
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فهرسة مساهمة: Keywords: 16S rRNA; 18S rRNA; Christmas tree; fir tree; metagenome; pH; rhizosphere-inhabiting microbes; soil acidification
المشرفين على المادة: 0 (Soil)
70FD1KFU70 (Sulfur)
تواريخ الأحداث: Date Created: 20210707 Date Completed: 20211229 Latest Revision: 20231020
رمز التحديث: 20231020
مُعرف محوري في PubMed: PMC8552644
DOI: 10.1128/Spectrum.00166-21
PMID: 34232064
قاعدة البيانات: MEDLINE
الوصف
تدمد:2165-0497
DOI:10.1128/Spectrum.00166-21