دورية أكاديمية
أعرض في EDS

Superresolved polarization-enhanced second-harmonic generation for direct imaging of nanoscale changes in collagen architecture.

التفاصيل البيبلوغرافية
العنوان: Superresolved polarization-enhanced second-harmonic generation for direct imaging of nanoscale changes in collagen architecture.
المؤلفون: Johnson PB; School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK.; Institute for Life Sciences, University of Southampton, Southampton, UK., Karvounis A; Optoelectronics Research Centre and Centre for Photonic Metamaterials, University of Southampton, Southampton, UK., Singh HJ; Physics and Astronomy, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK., Brereton CJ; NIHR Southampton Biomedical Research Centre, University Hospitals Southampton, Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK., Bourdakos KN; School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK.; Institute for Life Sciences, University of Southampton, Southampton, UK., Lunn K; Synairgen Research Ltd., Southampton, UK., Roberts JJW; Synairgen Research Ltd., Southampton, UK., Davies DE; Institute for Life Sciences, University of Southampton, Southampton, UK.; NIHR Southampton Biomedical Research Centre, University Hospitals Southampton, Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK., Muskens OL; Physics and Astronomy, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK., Jones MG; Institute for Life Sciences, University of Southampton, Southampton, UK.; NIHR Southampton Biomedical Research Centre, University Hospitals Southampton, Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, Southampton, UK., Mahajan S; School of Chemistry, Faculty of Engineering and Physical Sciences, University of Southampton, Southampton, UK.; Institute for Life Sciences, University of Southampton, Southampton, UK.
المصدر: Optica [Optica] 2021 May 13; Vol. 8 (5), pp. 674-685. Date of Electronic Publication: 2021 May 13 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Optica Publishing Group Country of Publication: United States NLM ID: 101643595 Publication Model: eCollection Cited Medium: Print ISSN: 2334-2536 (Print) Linking ISSN: 23342536 NLM ISO Abbreviation: Optica Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: <2022->: Washington, DC : Optica Publishing Group
Original Publication: Washington, DC : Optical Society of America, [2014]-
مستخلص: Superresolution (SR) optical microscopy has allowed the investigation of many biological structures below the diffraction limit; however, most of the techniques are hampered by the need for fluorescent labels. Nonlinear label-free techniques such as second-harmonic generation (SHG) provide structurally specific contrast without the addition of exogenous labels, allowing observation of unperturbed biological systems. We use the photonic nanojet (PNJ) phenomena to achieve SR-SHG. A resolution of ∼ λ / 6 with respect to the fundamental wavelength, that is, a ∼ 2.3 -fold improvement over conventional or diffraction-limited SHG under the same imaging conditions is achieved. Crucially we find that the polarization properties of excitation are maintained in a PNJ. This is observed in experiment and simulations. This may have widespread implications to increase sensitivity by detection of polarization-resolved SHG by observing anisotropy in signals. These new, to the best of our knowledge, findings allowed us to visualize biological SHG-active structures such as collagen at an unprecedented and previously unresolvable spatial scale. Moreover, we demonstrate that the use of an array of self-assembled high-index spheres overcomes the issue of a limited field of view for such a method, allowing PNJ-assisted SR-SHG to be used over a large area. Dysregulation of collagen at the nanoscale occurs in many diseases and is an underlying cause in diseases such as lung fibrosis. Here we demonstrate that pSR-SHG allows unprecedented observation of changes at the nanoscale that are invisible by conventional diffraction-limited SHG imaging. The ability to nondestructively image SHG-active biological structures without labels at the nanoscale with a relatively simple optical method heralds the promise of a new tool to understand biological phenomena and drive drug discovery.
Competing Interests: The authors declare no conflicts of interest.
(Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.)
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معلومات مُعتمدة: United Kingdom WT_ Wellcome Trust
تواريخ الأحداث: Date Created: 20210709 Latest Revision: 20240402
رمز التحديث: 20240402
مُعرف محوري في PubMed: PMC8237832
DOI: 10.1364/OPTICA.411325
PMID: 34239949
قاعدة البيانات: MEDLINE
الوصف
تدمد:2334-2536
DOI:10.1364/OPTICA.411325