دورية أكاديمية
Systematic mining of fungal chimeric terpene synthases using an efficient precursor-providing yeast chassis.
| العنوان: | Systematic mining of fungal chimeric terpene synthases using an efficient precursor-providing yeast chassis. |
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| المؤلفون: | Chen R; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China., Jia Q; Genome Science and Technology Graduate Program, University of Tennessee, Knoxville, TN 37996., Mu X; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China., Hu B; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China., Sun X; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China., Deng Z; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China.; Hubei Engineering Laboratory for Synthetic Microbiology, Wuhan Institute of Biotechnology, Wuhan 430075, People's Republic of China., Chen F; Genome Science and Technology Graduate Program, University of Tennessee, Knoxville, TN 37996; liutg@whu.edu.cn gkbian@whu.edu.cn fengc@utk.edu.; Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996., Bian G; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China; liutg@whu.edu.cn gkbian@whu.edu.cn fengc@utk.edu., Liu T; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Wuhan University School of Pharmaceutical Sciences, Wuhan 430071, People's Republic of China; liutg@whu.edu.cn gkbian@whu.edu.cn fengc@utk.edu.; Hubei Engineering Laboratory for Synthetic Microbiology, Wuhan Institute of Biotechnology, Wuhan 430075, People's Republic of China. |
| المصدر: | Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2021 Jul 20; Vol. 118 (29). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, DC : National Academy of Sciences |
| مواضيع طبية MeSH: | Alkyl and Aryl Transferases/*genetics , Fungal Proteins/*genetics , Mutant Chimeric Proteins/*genetics, Alkyl and Aryl Transferases/metabolism ; Diterpenes/chemistry ; Diterpenes/metabolism ; Evolution, Molecular ; Fungal Proteins/metabolism ; Fungi/classification ; Fungi/enzymology ; Fungi/genetics ; Genome, Fungal/genetics ; Molecular Structure ; Mutant Chimeric Proteins/metabolism ; Mutation ; Phylogeny ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Sesterterpenes/chemistry ; Sesterterpenes/metabolism |
| مستخلص: | Chimeric terpene synthases, which consist of C-terminal prenyltransferase (PT) and N-terminal class I terpene synthase (TS) domains (termed PTTSs here), is unique to fungi and produces structurally diverse di- and sesterterpenes. Prior to this study, 20 PTTSs had been functionally characterized. Our understanding of the origin and functional evolution of PTTS genes is limited. Our systematic search of sequenced fungal genomes among diverse taxa revealed that PTTS genes were restricted to Dikarya. Phylogenetic findings indicated different potential models of the origin and evolution of PTTS genes. One was that PTTS genes originated in the common Dikarya ancestor and then underwent frequent gene loss among various subsequent lineages. To understand their functional evolution, we selected 74 PTTS genes for biochemical characterization in an efficient precursor-providing yeast system employing chassis-based, robot-assisted, high-throughput automatic assembly. We found 34 PTTS genes that encoded active enzymes and collectively produced 24 di- and sesterterpenes. About half of these di- and sesterterpenes were also the products of the 20 known PTTSs, indicating functional conservation, whereas the PTTS products included the previously unknown sesterterpenes, sesterevisene (1), and sesterorbiculene (2), suggesting that a diversity of PTTS products awaits discovery. Separating functional PTTSs into two monophyletic groups implied that an early gene duplication event occurred during the evolution of the PTTS family followed by functional divergence with the characteristics of distinct cyclization mechanisms. Competing Interests: Competing interest statement: One patent application has been submitted based on the work reported here. (Copyright © 2021 the Author(s). Published by PNAS.) |
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| فهرسة مساهمة: | Keywords: chimeric terpene synthases; diterpene; evolution; fungi; sesterterpene |
| المشرفين على المادة: | 0 (Diterpenes) 0 (Fungal Proteins) 0 (Mutant Chimeric Proteins) 0 (Sesterterpenes) EC 2.5.- (Alkyl and Aryl Transferases) EC 2.5.1.- (terpene synthase) |
| تواريخ الأحداث: | Date Created: 20210714 Date Completed: 20211208 Latest Revision: 20211214 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8307374 |
| DOI: | 10.1073/pnas.2023247118 |
| PMID: | 34257153 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1091-6490 |
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| DOI: | 10.1073/pnas.2023247118 |