دورية أكاديمية
أعرض في EDS

Key Role for CRB2 in the Maintenance of Apicobasal Polarity in Retinal Pigment Epithelial Cells.

التفاصيل البيبلوغرافية
العنوان: Key Role for CRB2 in the Maintenance of Apicobasal Polarity in Retinal Pigment Epithelial Cells.
المؤلفون: Paniagua AE; Institute of Neurosciences of Castilla y León, IBSAL, Cell Biology and Pathology, University of Salamanca, Salamanca, Spain.; Stein Eye Institute and Department of Ophthalmology, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States., Segurado A; Institute of Neurosciences of Castilla y León, IBSAL, Cell Biology and Pathology, University of Salamanca, Salamanca, Spain., Dolón JF; Institute of Neurosciences of Castilla y León, IBSAL, Cell Biology and Pathology, University of Salamanca, Salamanca, Spain., Esteve-Rudd J; Stein Eye Institute and Department of Ophthalmology, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States., Velasco A; Institute of Neurosciences of Castilla y León, IBSAL, Cell Biology and Pathology, University of Salamanca, Salamanca, Spain., Williams DS; Stein Eye Institute and Department of Ophthalmology, David Geffen School of Medicine at UCLA, Los Angeles, CA, United States., Lillo C; Institute of Neurosciences of Castilla y León, IBSAL, Cell Biology and Pathology, University of Salamanca, Salamanca, Spain.
المصدر: Frontiers in cell and developmental biology [Front Cell Dev Biol] 2021 Jun 28; Vol. 9, pp. 701853. Date of Electronic Publication: 2021 Jun 28 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101630250 Publication Model: eCollection Cited Medium: Print ISSN: 2296-634X (Print) Linking ISSN: 2296634X NLM ISO Abbreviation: Front Cell Dev Biol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2013]-
مستخلص: Apicobasal polarity is essential for epithelial cell function, yet the roles of different proteins in its completion is not fully understood. Here, we have studied the role of the polarity protein, CRB2, in human retinal pigment epithelial (RPE) cells during polarization in vitro , and in mature murine RPE cells in vivo . After establishing a simplified protocol for the culture of human fetal RPE cells, we studied the temporal sequence of the expression and localization of polarity and cell junction proteins during polarization in these epithelial cells. We found that CRB2 plays a key role in tight junction maintenance as well as in cell cycle arrest. In addition, our studies in vivo show that the knockdown of CRB2 in the RPE affects to the distribution of different apical polarity proteins and results in perturbed retinal homeostasis, manifested by the invasion of activated microglial cells into the subretinal space. Together our results demonstrate that CRB2 is a key protein for the development and maintenance of a polarized epithelium.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Paniagua, Segurado, Dolón, Esteve-Rudd, Velasco, Williams and Lillo.)
References: J Cell Biol. 2005 Aug 29;170(5):813-23. (PMID: 16129788)
Front Immunol. 2019 Aug 20;10:1975. (PMID: 31481963)
Dev Dyn. 2011 Dec;240(12):2646-56. (PMID: 22072575)
Biochim Biophys Acta. 2008 Mar;1778(3):670-91. (PMID: 18201548)
J Neurophysiol. 1993 Oct;70(4):1669-80. (PMID: 8283222)
J Cell Sci. 2004 Aug 15;117(Pt 18):4169-77. (PMID: 15316081)
Stem Cell Res Ther. 2017 Oct 2;8(1):217. (PMID: 28969679)
Curr Opin Cell Biol. 2005 Oct;17(5):453-8. (PMID: 16098725)
Mol Biol Cell. 2004 Apr;15(4):1981-90. (PMID: 14718565)
Int J Cancer. 1983 Jan 15;31(1):13-20. (PMID: 6339421)
Prog Retin Eye Res. 2011 Sep;30(5):296-323. (PMID: 21704180)
Hum Mol Genet. 2013 Jan 1;22(1):35-50. (PMID: 23001562)
Neuron. 2010 Apr 15;66(1):69-84. (PMID: 20399730)
Nat Genet. 1999 Oct;23(2):217-21. (PMID: 10508521)
J Cell Biol. 2005 Feb 28;168(5):705-11. (PMID: 15738264)
Exp Eye Res. 2015 Jul;136:116-30. (PMID: 25952657)
Semin Cancer Biol. 2012 Jun;22(3):208-15. (PMID: 22465739)
Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6162-6. (PMID: 1631104)
Cancer Res. 2008 Jun 1;68(11):4105-15. (PMID: 18519669)
Nat Cell Biol. 2003 Feb;5(2):137-42. (PMID: 12545177)
Trends Cell Biol. 2011 Dec;21(12):727-35. (PMID: 21782440)
J Virol. 2003 Aug;77(16):8957-61. (PMID: 12885912)
Nat Cell Biol. 2003 Jan;5(1):12-4. (PMID: 12511884)
Eur J Neurosci. 2018 Jun;47(12):1444-1456. (PMID: 29738614)
Nat Rev Cancer. 2011 Dec 15;12(1):23-38. (PMID: 22169974)
Invest Ophthalmol Vis Sci. 1994 Sep;35(10):3582-8. (PMID: 8088949)
EMBO Mol Med. 2015 Jul 02;7(9):1179-97. (PMID: 26139610)
Biochim Biophys Acta. 2008 Mar;1778(3):614-30. (PMID: 18005931)
Nat Commun. 2014 Aug 01;5:4557. (PMID: 25080976)
Sci Rep. 2015 Dec 03;5:17699. (PMID: 26631503)
J Cell Biol. 2004 Oct 11;167(1):135-47. (PMID: 15479740)
J Neurosci. 2011 Nov 23;31(47):17230-41. (PMID: 22114289)
Invest Ophthalmol Vis Sci. 2006 Aug;47(8):3612-24. (PMID: 16877436)
Int J Oncol. 2015 Feb;46(2):657-66. (PMID: 25406012)
Mol Cell Neurosci. 2007 Mar;34(3):431-44. (PMID: 17223574)
Exp Eye Res. 2019 Mar;180:164-173. (PMID: 30593785)
Hum Mol Genet. 2010 Nov 1;19(21):4229-38. (PMID: 20709808)
Gene Ther. 2012 Feb;19(2):145-53. (PMID: 22052240)
Biol Cell. 2002 Oct;94(6):305-13. (PMID: 12500938)
Exp Eye Res. 2014 Sep;126:38-45. (PMID: 24090540)
Nature. 2000 Feb 10;403(6770):676-80. (PMID: 10688207)
Gene. 1996 Oct 17;176(1-2):237-42. (PMID: 8918259)
Mol Cell Biol. 2014 Jan;34(1):43-56. (PMID: 24164893)
Nat Rev Mol Cell Biol. 2014 Apr;15(4):225-42. (PMID: 24651541)
Brain Res. 2006 Sep 19;1110(1):55-63. (PMID: 16859655)
Invest Ophthalmol Vis Sci. 2011 Mar 14;52(3):1392-403. (PMID: 21071746)
Mol Vis. 2005 Dec 28;11:1183-99. (PMID: 16402019)
J Cell Sci. 2005 Jul 1;118(Pt 13):2859-69. (PMID: 15976445)
Mol Biol Cell. 2004 Mar;15(3):1324-33. (PMID: 14718572)
Prog Retin Eye Res. 2020 Feb 24;:100846. (PMID: 32105772)
Invest Ophthalmol Vis Sci. 2011 Nov 04;52(12):8614-20. (PMID: 21960553)
Front Biosci (Landmark Ed). 2009 Jan 01;14:2149-69. (PMID: 19273190)
Sci Rep. 2015 Sep 25;5:14504. (PMID: 26404741)
Cell Mol Life Sci. 2016 Sep;73(18):3535-53. (PMID: 27151512)
J Cell Sci. 2003 Jul 15;116(Pt 14):2895-906. (PMID: 12771187)
Nat Rev Mol Cell Biol. 2003 Mar;4(3):225-36. (PMID: 12612641)
Nat Protoc. 2009;4(5):662-73. (PMID: 19373231)
Mol Vis. 2001 Feb 07;7:14-9. (PMID: 11182021)
Mol Cell Biol. 2015 Oct;35(19):3423-35. (PMID: 26217016)
Curr Biol. 2020 Jul 20;30(14):2791-2804.e6. (PMID: 32531288)
Stem Cells. 2011 Feb;29(2):193-205. (PMID: 21732478)
معلومات مُعتمدة: P30 EY000331 United States EY NEI NIH HHS; R01 EY027442 United States EY NEI NIH HHS; R21 EY031109 United States EY NEI NIH HHS
فهرسة مساهمة: Keywords: CRB2; Crb complex; RPE-retinal pigment epithelium; cell junctions; cell polarity; differentiation; epithelium; retina
تواريخ الأحداث: Date Created: 20210715 Latest Revision: 20240619
رمز التحديث: 20240619
مُعرف محوري في PubMed: PMC8273544
DOI: 10.3389/fcell.2021.701853
PMID: 34262913
قاعدة البيانات: MEDLINE
الوصف
تدمد:2296-634X
DOI:10.3389/fcell.2021.701853