دورية أكاديمية
Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants.
| العنوان: | Detection and Quantification of Secreted Nuclease Activity in Staphylococcus aureus Culture Supernatants. |
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| المؤلفون: | Wiemels RE; Department of Biological Sciences, Ohio University, Athens, OH, USA., Keogh RA; Department of Biological Sciences, Ohio University, Athens, OH, USA., Carroll RK; Department of Biological Sciences, Ohio University, Athens, OH, USA. carrolr3@ohio.edu. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2341, pp. 17-24. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Bacterial Proteins/*analysis , Micrococcal Nuclease/*analysis , Salmon/*genetics , Staphylococcus aureus/*enzymology, Animals ; Bacterial Proteins/metabolism ; Bacteriological Techniques ; Extracellular Traps/metabolism ; Immune Evasion ; Male ; Micrococcal Nuclease/metabolism ; Spermatozoa/chemistry ; Staphylococcus aureus/pathogenicity ; Virulence Factors/analysis ; Virulence Factors/metabolism |
| مستخلص: | Staphylococcal secreted nuclease contributes to S. aureus virulence by degrading neutrophil extracellular traps (NETs), which allows the bacterium to evade the host immune system and has also been shown to promote biofilm dispersal. In this chapter, two methods for detecting nuclease activity are described, both of which have increased sensitivity compared to the traditional nuclease agar method. (© 2021. Springer Science+Business Media, LLC, part of Springer Nature.) |
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| معلومات مُعتمدة: | R21 AI128376 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: FRET; Nuclease; Salmon sperm DNA; Staphylococcus; Virulence factor |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Virulence Factors) 0 (nuc protein, staphylococcus) EC 3.1.31.1 (Micrococcal Nuclease) |
| تواريخ الأحداث: | Date Created: 20210715 Date Completed: 20210825 Latest Revision: 20230503 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10152981 |
| DOI: | 10.1007/978-1-0716-1550-8_3 |
| PMID: | 34264456 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-1550-8_3 |