دورية أكاديمية
أعرض في EDS

Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440.

التفاصيل البيبلوغرافية
العنوان: Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440.
المؤلفون: Werner AZ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA; BOTTLE Consortium, Golden, CO, USA., Clare R; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA; BOTTLE Consortium, Golden, CO, USA., Mand TD; BOTTLE Consortium, Golden, CO, USA; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Pardo I; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA; BOTTLE Consortium, Golden, CO, USA., Ramirez KJ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA; BOTTLE Consortium, Golden, CO, USA., Haugen SJ; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA., Bratti F; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA; BOTTLE Consortium, Golden, CO, USA., Dexter GN; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Elmore JR; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Huenemann JD; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Peabody GL 5th; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA., Johnson CW; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA; BOTTLE Consortium, Golden, CO, USA., Rorrer NA; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA; BOTTLE Consortium, Golden, CO, USA., Salvachúa D; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA; BOTTLE Consortium, Golden, CO, USA., Guss AM; BOTTLE Consortium, Golden, CO, USA; Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA. Electronic address: gussam@ornl.gov., Beckham GT; Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, CO, USA; BOTTLE Consortium, Golden, CO, USA. Electronic address: Gregg.Beckham@nrel.gov.
المصدر: Metabolic engineering [Metab Eng] 2021 Sep; Vol. 67, pp. 250-261. Date of Electronic Publication: 2021 Jul 12.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: Belgium NLM ID: 9815657 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-7184 (Electronic) Linking ISSN: 10967176 NLM ISO Abbreviation: Metab Eng Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Brugge, Belgium ; Orlando, FL : Academic Press, c1999-
مواضيع طبية MeSH: Polyethylene Terephthalates* , Pseudomonas putida*/genetics, Adipates ; Burkholderiales ; Ethylenes ; Hydrolases ; Phthalic Acids ; Rhodococcus
مستخلص: Poly(ethylene terephthalate) (PET) is the most abundantly consumed synthetic polyester and accordingly a major source of plastic waste. The development of chemocatalytic approaches for PET depolymerization to monomers offers new options for open-loop upcycling of PET, which can leverage biological transformations to higher-value products. To that end, here we perform four sequential metabolic engineering efforts in Pseudomonas putida KT2440 to enable the conversion of PET glycolysis products via: (i) ethylene glycol utilization by constitutive expression of native genes, (ii) terephthalate (TPA) catabolism by expression of tphA2 II A3 II B II A1 II from Comamonas and tpaK from Rhodococcus jostii, (iii) bis(2-hydroxyethyl) terephthalate (BHET) hydrolysis to TPA by expression of PETase and MHETase from Ideonella sakaiensis, and (iv) BHET conversion to a performance-advantaged bioproduct, β-ketoadipic acid (βKA) by deletion of pcaIJ. Using this strain, we demonstrate production of 15.1 g/L βKA from BHET at 76% molar yield in bioreactors and conversion of catalytically depolymerized PET to βKA. Overall, this work highlights the potential of tandem catalytic deconstruction and biological conversion as a means to upcycle waste PET.
(Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
التعليقات: Erratum in: Metab Eng. 2024 Jul 17:S1096-7176(24)00094-6. doi: 10.1016/j.ymben.2024.07.004. (PMID: 39029782)
فهرسة مساهمة: Keywords: Bio-upcycling; MHETase; Metabolic engineering; PETase; Plastics upcycling; Terephthalic acid
المشرفين على المادة: 0 (Adipates)
0 (Ethylenes)
0 (Phthalic Acids)
0 (Polyethylene Terephthalates)
1379JRA56F (3-oxoadipic acid)
6S7NKZ40BQ (terephthalic acid)
EC 3.- (Hydrolases)
SCR Organism: Ideonella sakaiensis; Rhodococcus jostii
تواريخ الأحداث: Date Created: 20210715 Date Completed: 20211124 Latest Revision: 20240719
رمز التحديث: 20240720
DOI: 10.1016/j.ymben.2021.07.005
PMID: 34265401
قاعدة البيانات: MEDLINE
الوصف
تدمد:1096-7184
DOI:10.1016/j.ymben.2021.07.005