دورية أكاديمية
أعرض في EDS

Flow Cytometric Assessment of Ki-67 Expression in Lymphocytes From Physiologic Lymph Nodes, Lymphoma Cell Populations and Remnant Normal Cell Populations From Lymphomatous Lymph Nodes.

التفاصيل البيبلوغرافية
العنوان: Flow Cytometric Assessment of Ki-67 Expression in Lymphocytes From Physiologic Lymph Nodes, Lymphoma Cell Populations and Remnant Normal Cell Populations From Lymphomatous Lymph Nodes.
المؤلفون: Rütgen BC; Clinical Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Baumgartner D; Clinical Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Fuchs-Baumgartinger A; Institute of Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Rigillo A; Department of Veterinary Medical Sciences, University of Bologna, Bologna, Italy., Škor O; Clinic for Internal Medicine, Department for Small Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria., Hammer SE; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Saalmüller A; Institute of Immunology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria., Schwendenwein I; Clinical Pathology, Department of Pathobiology, University of Veterinary Medicine Vienna, Vienna, Austria.
المصدر: Frontiers in veterinary science [Front Vet Sci] 2021 Jun 29; Vol. 8, pp. 663656. Date of Electronic Publication: 2021 Jun 29 (Print Publication: 2021).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101666658 Publication Model: eCollection Cited Medium: Print ISSN: 2297-1769 (Print) Linking ISSN: 22971769 NLM ISO Abbreviation: Front Vet Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2014]-
مستخلص: Recent literature suggests conventional flow cytometric (FCM) immunophenotyping complemented by Ki-67 FCM assessment as a reliable tool to classify canine lymphomas. Ki-67 expression assessed by FCM is rarely reported in canine lymphoma cases and reference data for normal canine lymph nodes are missing. Moreover, nothing is known about the Ki-67 expression within the occasionally observed remnant cell population within the gates of normal lymphocytes in lymphoma cases. Aim of this study was to compare flow cytometric Ki-67 expression of lymphocyte populations from normal canine lymph nodes, lymphoma cells from World-Health-Organisation (WHO) classified lymphoma patient samples and their neighboring normal remnant cell population. Cryopreserved lymphocyte cell suspensions from normal lymph nodes from eight dogs free of lymphoma served as reference material. Fourteen cases diagnosed by cytology, FCM, clonality testing, histopathology including immunohistochemistry consisting of 10 DLBCL, 1 MZL, 1 PTCL and 2 TZL showed a residual small lymphocyte population and were investigated. The Ki-67 expression in normal canine lymphoid tissue was 3.19 ± 2.17%. Mean Ki-67 expression in the malignant cell populations was 41 ± 24.36%. Ki-67 positivity was 12.34 ± 10.68% in the residual physiologic lymphocyte population, which otherwise exhibited a physiologic immunophenotype pattern. This ratio was equivalent ( n = 3) or lower ( n = 11) than the Ki-67 expression of the malignant cell population within the sample. This is the first report of FCM derived Ki-67 expression combined with immunophenotype patterns in normal canine lymph nodes, compared with lymphoma cell populations and residual normal cell populations of lymphoma cases diagnosed by state of the art technology.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2021 Rütgen, Baumgartner, Fuchs-Baumgartinger, Rigillo, Škor, Hammer, Saalmüller and Schwendenwein.)
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فهرسة مساهمة: Keywords: Ki-67; canine lymphoma; flow cytometry; immunophenotyping; reference data
تواريخ الأحداث: Date Created: 20210716 Latest Revision: 20240812
رمز التحديث: 20240813
مُعرف محوري في PubMed: PMC8276100
DOI: 10.3389/fvets.2021.663656
PMID: 34268346
قاعدة البيانات: MEDLINE
الوصف
تدمد:2297-1769
DOI:10.3389/fvets.2021.663656