دورية أكاديمية
أعرض في EDS

Counting Enchytraeus crypticus Juveniles in Chronic Exposures: An Alternative Method for Ecotoxicity Studies Using Tropical Artificial Soil.

التفاصيل البيبلوغرافية
العنوان: Counting Enchytraeus crypticus Juveniles in Chronic Exposures: An Alternative Method for Ecotoxicity Studies Using Tropical Artificial Soil.
المؤلفون: Felipe MC; Biological Processes Laboratory (LPB), Department of Hydraulic and Sanitation, São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, Brazil. mayarafelipe@usp.br., Bernegossi AC; Aquatic Ecology Laboratory (LEAA), Department of Hydraulic and Sanitation, São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, Brazil., Pinheiro FR; Aquatic Ecology Laboratory (LEAA), Department of Hydraulic and Sanitation, São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, Brazil., Castro GB; Aquatic Ecology Laboratory (LEAA), Department of Hydraulic and Sanitation, São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, Brazil., Moura L; Aquatic Ecology Laboratory (LEAA), Department of Hydraulic and Sanitation, São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, Brazil., Zaiat M; Biological Processes Laboratory (LPB), Department of Hydraulic and Sanitation, São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, Brazil., Corbi JJ; Aquatic Ecology Laboratory (LEAA), Department of Hydraulic and Sanitation, São Carlos School of Engineering (EESC), University of São Paulo (USP), Av. João Dagnone, 1100, Santa Angelina, São Carlos, Brazil.
المصدر: Bulletin of environmental contamination and toxicology [Bull Environ Contam Toxicol] 2021 Sep; Vol. 107 (3), pp. 494-499. Date of Electronic Publication: 2021 Jul 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Verlag Country of Publication: United States NLM ID: 0046021 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0800 (Electronic) Linking ISSN: 00074861 NLM ISO Abbreviation: Bull Environ Contam Toxicol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York : Springer Verlag
مواضيع طبية MeSH: Oligochaeta* , Soil Pollutants*/analysis , Soil Pollutants*/toxicity, Animals ; Ecotoxicology ; Reproduction ; Soil ; Toxicity Tests
مستخلص: Soil toxicity tests are commonly applied using Enchytraeus crypticus to analyze reproductive outputs. However, the traditional method for counting potworms takes a long time due to the significant number of offspring. This paper compares the conventional total counting of E. crypticus juveniles (M1) and an alternative methodology (M2). The proposed methodology (M2) uses a simple random counting method (1/4) for the partial counting of juveniles and total estimation. Chronic bioassays (21 days of exposure) were performed in tropical artificial soil (TAS) using sugarcane vinasse as a hazardous substance. Comparing the final density of juveniles recorded in M1 and M2, no statistical differences were pointed out in either one. Applying analyses based on effective concentration (EC10 and EC50), no statistical differences were identified there either. The t-test showed that there was no statistical difference between the counting methods (M1 and M2) in each treatment (control and dilutions). Moreover, we ran the Tukey test for M1 and M2 methods separately and observed that 100 % of the vinasse showed a statistical difference compared to the control treatment in both (p ≤ 0.05), affirming that independent of the counting method, the ecotoxicological outputs were similar. Therefore, the proposed alternative is a suitable method for bioassay using. E. crypticus in tropical artificial soil, decreasing to 1/4 the total time required for counting.
(© 2021. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
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معلومات مُعتمدة: 2018/21901-0 São Paulo Research Foundation; 2015/06246-7 São Paulo Research Foundation; 131610/2018-0 National Council for Scientific and Technological Development; 88887.353028/2019-00 Brazilian Coordination for the Improvement of Higher Education Personnel; 88887.352964/2019-00 Brazilian Coordination for the Improvement of Higher Education Personnel; 88887.339518/2019-00 Brazilian Coordination for the Improvement of Higher Education Personnel
فهرسة مساهمة: Keywords: Counting juveniles; Enchytraeid; Potworms; Soil toxicity; Time optimization
المشرفين على المادة: 0 (Soil)
0 (Soil Pollutants)
تواريخ الأحداث: Date Created: 20210716 Date Completed: 20210826 Latest Revision: 20220218
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC8284039
DOI: 10.1007/s00128-021-03330-4
PMID: 34269820
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-0800
DOI:10.1007/s00128-021-03330-4