دورية أكاديمية
Induced-damages on preantral follicles by withanolide D, a potent chemotherapy candidate are not attenuated by melatonin.
| العنوان: | Induced-damages on preantral follicles by withanolide D, a potent chemotherapy candidate are not attenuated by melatonin. |
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| المؤلفون: | Palomino GJQ; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, CEP: 60714-903, Brazil., Sá NAR; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, CEP: 60714-903, Brazil., Guerreiro DD; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, CEP: 60714-903, Brazil., Gomes FDR; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, CEP: 60714-903, Brazil., da Silva RF; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, CEP: 60714-903, Brazil., Lopes EPF; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, CEP: 60714-903, Brazil., Paes VM; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, CEP: 60714-903, Brazil., Gataí PHS; Laboratory of Phytochemical Analysis of Medicinal Plants (LAFIPLAN), Brazil., Alves BG; Postgraduate Program in Animal Bioscience, Federal University of Goias, Jatai, GO, Brazil., Pessoa ODL; Laboratory of Phytochemical Analysis of Medicinal Plants (LAFIPLAN), Brazil., Figueiredo JR; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, CEP: 60714-903, Brazil., Rocha RMP; University of International Integration of Afro-Brazilian Lusophony (UNILAB), Brazil., Rodrigues APR; Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceará, Av. Dr. Silas Munguba, 1700, Fortaleza, CE, CEP: 60714-903, Brazil. Electronic address: aprrodriguespapers@gmail.com. |
| المصدر: | Reproductive toxicology (Elmsford, N.Y.) [Reprod Toxicol] 2021 Sep; Vol. 104, pp. 125-133. Date of Electronic Publication: 2021 Jul 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pergamon In Cooperation With The Reproductive Toxicology Center Country of Publication: United States NLM ID: 8803591 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-1708 (Electronic) Linking ISSN: 08906238 NLM ISO Abbreviation: Reprod Toxicol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Elmsford Ny : Pergamon In Cooperation With The Reproductive Toxicology Center Original Publication: Elmsford, N.Y., U.S.A. : Pergamon Press, c1987- |
| مواضيع طبية MeSH: | Antineoplastic Agents/*toxicity , Melatonin/*pharmacology , Protective Agents/*pharmacology , Withanolides/*toxicity, Animals ; Culture Media ; Female ; Oocytes ; Ovarian Follicle |
| مستخلص: | Withanolide D (WD) has been investigated as an antineoplastic drug. This study aimed to evaluate whether melatonin (MT) could attenuate toxic effects on preantral follicles enclosed in the ovarian cortex (experiment 1 - E1) or on isolated secondary follicles (experiment 2 - E2) exposed to WD. For E1, ovarian cortex was incubated for 48 h to: (1) α-MEM + ; (2) α-MEM + plus 6 μM WD; (3) α-MEM + plus 3 mmol/L MT or (4) α-MEM + plus WD and MT. For E2, secondary follicles were exposed for until 96 h in. (1) only to basic medium (α-MEM ++ /α-MEM ++ ); (2) α-MEM ++ plus 3 mmol/L MT (MT/MT); (3) α-MEM ++ until 48 h, followed by more 48 h in 6 μM WD (α-MEM ++ /WD) or (4) a pre-exposure to MT for until 48 h, followed by more 48 h of exposure to WD plus MT (MT/MT + WD). The main results obtained showed that exposure to drugs caused damage to follicular morphology (WD or WD + MT) and diameter (WD) in the ovarian cortex or in isolated follicles. In pre-antral follicles in situ, ATM expression increased in the presence of WD, MT or association. As for the secondary follicles, ATM and γH2AX were immunostained in the granulosa and theca cells and oocytes in all treatments. TAp63α was immunostained in follicles included in the ovarian cortex and in isolated follicles. We conclude that melatonin did not provide protection and could have enhanced the toxic effect of WD to follicles surrounded or not by the ovarian cortex. (Copyright © 2021 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; Chemotherapy; Infertility; Phytotherapy; Preantral follicles |
| المشرفين على المادة: | 0 (Antineoplastic Agents) 0 (Culture Media) 0 (Protective Agents) 0 (Withanolides) 30655-48-2 (withanolide D) JL5DK93RCL (Melatonin) |
| تواريخ الأحداث: | Date Created: 20210718 Date Completed: 20211217 Latest Revision: 20211217 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.reprotox.2021.07.005 |
| PMID: | 34274432 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-1708 |
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| DOI: | 10.1016/j.reprotox.2021.07.005 |